شناسایی ترکیبات شیمیایی تشکیل دهنده اسانس و بررسی اثرات آنتی‌اکسیدانی آنغوزه ایرانی و افغانستانی

نویسندگان
حمزه امیری
پروانه همتی حسن گاویار
سید کاظم حسن زاده
گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه لرستان، خرم آباد، ایران
10.22034/FSCT.21.152.51
چکیده


در این مطالعه ترکیبات تشکیل دهنده اسانس، اثرات آنتی اکسیدانی و میزان فنل و فلاونوئید آنغوزه در دو جمعیت ایرانی و افغانستانی مورد بررسی قرار گرفته است. شناسایی ترکیب های تشکیل دهنده اسانس توسط دستگاه GC-MS صورت گرفت. بررسی اثرات آنتی اکسیدانی با استفاده از روش‌های 2و2 دی فنیل 1-پیکریل هیدرازیل و بتاکاروتن - لینولئیک اسید انجام شد. محتوای ترکیبات فنلی و فلاونوئیدی نیز به ترتیب با استفاده از استانداردهای گالیک اسید و کوئرستین اندازه گیری شد. نتایج نشان داد که پروپنیل سک بوتیل دی­سولفید (72%/42)، استون دی متیل مرکاپتول(84%/14) و گاما ادیسمول (8.60%) فراوان ترین ترکیبات موجود در اسانس آنغوزه ایرانی است، پروپنیل سک بوتیل دی سولفید (61%/39)، استون و دی متیل مرکاپتول (25%/18)، و 8 اتیل پنتادی دکان (97%/17) فراوان ترین ترکیبات تشکیل دهنده اسانس آنغوزه افغانستانی بودند. میزان فعالیت آنتی اکسیدانی انغوزه ایرانی در روش DPPH قدرت بیشتری را نشان داد در حالی که در روش بتاکاروتن- لینولئیک اسید میزان فعالیت آنتی­اکسیدانی نمونه افغانستانی بیشتر بود. محتوای ترکیبات فنلی و فلاونوئیدی در جمعیت افغانستانی بیشتر از جمعیت ایرانی بود. در مجموع نتایج نشان داد که دو جمعیت مورد بررسی در این پژوهش از نظر نوع و درصد ترکیبات تشکیل دهنده اسانس، قدرت آنتی اکسیدانی و محتوای فنل و فلاونوئید دارای تفاوت هایی با هم هستند. این تفاوت‌ها احتمالاً ناشی از تفاوت در عوامل اکولوژیکی مانند زیستگاه های محل رویش این جمعیت ها و تاثیر این عوامل برفاکتورهای مطالعه شده است.
کلیدواژه‌ها
پروپنیل سک بوتیل دی‌سولفید
استون دی متیل مرکاپتول
DPPH
بتاکاروتن- لینولئیک اسید

موضوعات

عنوان مقاله English

Chemical composition of essential oil and antioxidant activity of Iranian and Afghanestanian population of Ferula assa-foetida L

نویسندگان English

Hamzeh Amiri
Parvaneh Hemmati Hassan Gavyar
Seyyed Kazem Hassanzadeh
Department of Biology, Faculty of Science, Lorestan University, Khoram-Abad, Iran
چکیده English

In this study, the essential oil composition, antioxidant activities, phenolic and flavonoid contents, and of metanolic extract of Iranian and Afghanestanian populations of Ferula assa-foetida were evaluated. Essential oils were analyzed by using GC and GC/MS, The antioxidant activity were measured by 2, 2-diphenyl-1-picrylhydrazyl and β-carotene/linoleic acid assay, also phenol and flavonoid content were measured by gallic acid and quercetin as standard compound. Result showed that propenyl sec butyl disulfide (42.72%), acetone, dimethyl mercaptole (14.84%) and gamma- eudesmol (8.6%) were the main compouned found in the Iranian population while; the Afghanestanian population were rich of propenyl sec butyl disulfide (39.61%), acetone, dimethyl mercaptole (18.25%) and 8-ethyl-pentathiadecane (18.97%). In DPPH and β-carotene/linoleic acid tests, Iranian and Afghanestanian populations have stronger antioxidant activity respectively, also, the results demonstrated that the Afghanestanian population have more phenolic and flavonoid contents than the Iranian population. Overall, the results showed that the two populations investigated in this research have differences in terms of the type and percentage of essential oil compounds, antioxidant activity and phenol and flavonoid content. These differences are probably caused by differences in ecological factors such as the habitats of these populations and the influence of these factors on the studied parameters.

Key words: propenyl sec-butyl disulfide, dimethyl mercaptol acetone, DPPH, β-carotene/linoleic acid

کلیدواژه‌ها English

propenyl sec-butyl disulfide
dimethyl mercaptol acetone
DPPH
β-carotene/linoleic acid
[1] Li, X.; Wang, Z. (2009) Chemical composition, antimicrobial and antioxidant activities of the essential oil in leaves of Salvia miltiorrhiza Bunge, Journal of Essential Oil Research, 5, 476-488.
[2] Hashemi, Z., Hojjati, M., & Tahanejad, M. (2015). Evaluation of antioxidant activity of essential oil extracted from Ferula gummosa Boiss in deep oil frying. Iranian Food Science and Technology Research Journal, 11(5), 631-642.‌ (in persian).
[3] Hemmati Hassan Gavyar, P., & Amiri, H. (2018). Chemical composition of essential oil and antioxidant activity of leaves and stems of Phlomis lurestanica. International Journal of Food Properties, 21(1), 1414-1422.‌
[4] [Kiokias, S., Varzakas, T., & Oreopoulou, V. )2008(. In vitro activity of vitamins, flavanoids, and natural phenolic antioxidants against the oxidative deterioration of oil-based systems. Critical Reviews in Food Science and Nutrition, 48, 78–93.
[5] Shahidi, F., & Ambigaipalan, P. (2015). Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects–A review. Journal of functional foods, 18, 820-897.‌
[6] Amini, H., Naghavi, M., Iranshahi, M., Yazdanfar, N., & Nasiri, J. (2019). Chemical composition of different parts of Ferula assa-foetida L. using GC-MS. Iranian Journal of Field Crop Science, 50(3), 89-96.‌ (in persian).
[7] Bahrami, G., Soltani, R., Sajjadi, S. E., Kanani, M. R., Naderi, R., Ghiasvand, N., & Shokoohinia, Y. (2013). Essential oil composition of Ferula assa-foetida L. fruits from Western Iran. Journal of Reports in Pharmaceutical Sciences, 2(2), 90-97.‌
[8] Nasiri Bezenjani, S., Razavizadeh, R., & Oloumi, H. (2017). Evaluation of content of phenylpropanoid compounds of latex and chemical composition of essential oil of Ferula assa-foetida L. in some natural pasturelands of Kerman, Iran. Journal of Plant Research (Iranian Journal of Biology), 30(3), 674-687. (in persian).
[9] Nazari, Z. E., & Iranshahi, M. (2011). Biologically active sesquiterpene coumarins from Ferula species. Phytotherapy research, 25(3), 315-323.‌
[10] Adams, R., 2001. Identification of essential oil compounds by gas chromatography/ quadrupole mass spectroscopy Carol Stream. Allured Pub, Corp., USA.
[11] Tepe, B.; Sokmen, M.; Akpulat, H.A.; Sokmen, A. (2006). Screening of the antioxidant potentials of six Salvia species from Turkey, Food Chemistry, 97, 200-204.
[12] Amiri, H. )2009(. The in vitro antioxidative properties of the essential oils and methanol extracts of Satureja macrosiphonia Bornm. J. Natural. Product. Research. 25, 232-243.
[13] Karamian, R.; Azizi, A.; Asadbegy, M; Pakzad, R. (2014) Essential oil composition and antioxidant activity of the methanol extracts of three Phlomis species from Iran. Journal of Biologically Active Products from Nature, 4, 343-353.
[14] Khajeh, M., Yamini, Y., Bahramifar, N., Sefidkon, F., & Pirmoradei, M. R. (2005). Comparison of essential oils compositions of Ferula assa-foetida obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food chemistry, 91(4), 639-644.
[15] Abyar, S., fakheri, B A., & mahdi nazhad, N. (2019). Ecological factors affecting the South West of the country on the gum and essential oil composition ferula asa foetida. Plant Process and Function, 8 (30) :125-135. (in persian).
[16] Kroymann, J. Donnerhacke, S. Schnabelrauch, D. & Mitchell-Olds, T. (2003). Evolutionary dynamics of an Arabidopsis insect resistance quantitative trait locus. Proceedings of the National Academy of Sciences of the USA, 100: 14587-14592.
[17] Amarowicz, R., Pegg, R. B., Rahimi-Moghaddam, P., Barl, B., & Weil, J. A. (2004). Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chemistry, 84(4), 551-562.‌
[18] Deveci, E., Tel-Çayan, G., & Duru, M. E. (2018). Phenolic profile, antioxidant, anticholinesterase, and anti-tyrosinase activities of the various extracts of Ferula elaeochytris and Sideritis stricta. International journal of food properties, 21(1), 771-783.
[19] Bertome, J. Isabel Arrillage, M. & Segura, J. (2007). Essential oil variation whitin and among natural population of Lavandula latifolia and its relation to their ecological areas. Biochemical Systematics and Ecology, (35): 479-488.
[20] Ahmadvand, H., Amiri, H., Dehghani Elmi, Z., & Bagheri, S. H. (2013). Chemical composition and antioxidant properties of Ferula-assa-foetida leaves essential oil. International Journal of Powertrains, 12(2), 52-57.‌
[21] Vahabil., Shahanipourk., & Monajemi, R, (2014). Short Paper: Antioxidant evaluation and phenolic contents in different extracts of Ferula assa-foetida resin in South Khorasan province, ECO phytochemistry of medicinal plants, 1(4), 89-100. (in persian).
[22] Mehrpour, M., Kashefi, B., & Moghadam, M. (2016). Evaluation of phytochemical and antioxidant activity in different parts of Ferula assafoetida L. from Semnan and Khorasan provinces, ECO phytochemistry of medicinal plants, 4(1), 56-68. .‌ (in persian).
[23] Narimani, R., Tarakemeh, A., Moghaddam, M., & Mahmoodi Sourestani, M. (2021). Phytochemical variation within aerial parts of ferula cupularis populations, an endangered medicinal plant from Iran. Chemistry & Biodiversity, 18(12), e2100551.‌
[24] Esmaeilzadeh Bahabadi, S., &Yousefzaei, F. (2019). Phytochemichl and antioxidant analysis of different parts of Citrullus colocynthis L. in different regions from Southeast of Iran. Eco-phytochemical Journal of Medicinal Plants, 7(3), 43-52.‌ (in persian).