بررسی حضور ژن تراریخته camv35s در شیر خشک و غذای کودک موجود در تهران با تکنیک real-time PCR

نویسندگان
رامین بهشتی زاده 1
شیلا صفاییان 1
الهام مسلمی 2
کسری اصفهانی 3
رضوان موسوی ندوشن 1
1 گروه علوم غذایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
2 گروه زیست شناسی، واحد تهران شرق، دانشگاه آزاد اسلامی، تهران، ایران
3 گروه فرآورده های زیستی گیاهی، پژوهشکده بیوتکنولوژی کشاورزی (IAB)، پژوهشکده ملی مهندسی ژنتیک و بیوتکنولوژی (NIGEB)، تهران، ایران
10.22034/FSCT.19.133.167
چکیده
ذرت و سویا بیشترین سطح کشت تراریختگی را در دنیا به خود اختصاص داده اند. با توجه به افزایش واردات سویا و ذرت به ایران و همچنین به دلیل استفاده از روغن های محصولات استراتژی در شیر خشک و غذای کودک، لذا ردیابی تراریختگی در مواد غذایی فرآوری شده با تکنیک Real-time PCR یک نیاز اساسی می باشد. ابتدا 40 نمونه غذای کودک و شیر خشک از داروخانه ها و سوپر مارکت های شهر تهران جمع آوری شد. همه نمونه با استفاده از کیت DNA آزما اکسیر پژوه استخراج شدند و بررسی کمی غلظت اسید نوکلئیک با دستگاه نانودراپ انجام شد. سپس برای بررسی کیفی استخراخ DNA انجام شده، تست PCR ژن های کنترل داخلی برای سویا(Lectin) و ذرت(Zein) گذاشته شدند. سپس با تکنیک Real-time PCR حضور ژن تراریخته CaMV35S بررسی گردید و نتایج به دست آمده از حضور ژن تراریخته در 2.5 ٪ نمونه غذای کودک و 10 ٪ نمونه در شیر خشک را نشان دادند. بنابراین در این مقاله میزان نفوذ ژن تراریخته در غذای کودک و شیر خشک مورد بررسی قرار گرفته است.
کلیدواژه‌ها
ردیابی
شیر خشک
تراریخته
Real-time PCR

موضوعات

عنوان مقاله English

Investigation of the CaMV35s gene in baby food and infant formula in Tehran by real-time PCR technique

نویسندگان English

ramin beheshti zadeh 1
shila Safaeian 1
elham moslemi 2
Kasra Esfahani 3
Rezvan Mousavi nadushan 1
1 Department of Food Science, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
3 Department of Plant Bioproducts, Institute of Agricultural Biotechnology (IAB), National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
چکیده English

Corn and soybeans have the largest area of ​​GM crops in the world. Milk powder and baby food are processed foods that contain corn and soybeans. Therefore, tracking transgenic corn and soy in processed food is one of the research problems. First, 40 samples of baby food and milk powder were collected from pharmacies and supermarkets in Tehran. All the samples were extracted using Azmaelixir DNA kit, and the nucleic acid concentration was checked with a nanodrop device, and internal control genes for soy (Lectin) and corn (Zein) were checked to ensure the extraction. Then, the presence of CaMV35S transgenic gene was checked by Real-time PCR technique. The results showed the presence of transgenic genes in 2.5% of baby food samples and 10% of samples in infant formula. Therefore, in this article, the amount of transgenic gene penetration in baby food and infant formula has been investigated.

کلیدواژه‌ها English

Detection
Real-time PCR
GMO
Infant formula
[1] Chawla, H., 2011. Introduction to plant biotechnology (3/e). CRC Press.
[2] Hediger, M.L., Overpeck, M.D., Ruan, W.J. and Troendle, J.F., 2000. Early infant feeding and growth status of US-born infants and children aged 4–71 mo: analyses from the third National Health and Nutrition Examination Survey, 1988–1994. The American journal of clinical nutrition, 72(1), pp.159-167.
[3] Pickett-Bernard, D., 2006. Infant Formula: Evaluating the Safety of New Ingredients. Journal of Human Lactation, 22(2), pp.231-232.
[4] Berdichevets, I.N., Shimshilashvili, H.R., Gerasymenko, I.M., Sindarovska, Y.R., Sheludko, Y.V. and Goldenkova-Pavlova, I.V., 2010. Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and bioanalytical chemistry, 397(6), pp.2289-2293.
[5] Arun, Ö.Ö., Yılmaz, F. and Muratoğlu, K., 2013. PCR detection of genetically modified maize and soy in mildly and highly processed foods. Food Control, 32(2), pp.525-531.
[6] Marmiroli, N., Maestri, E., Gulli, M., Malcevschi, A., Peano, C., Bordoni, R. and De Bellis, G., 2008. Methods for detection of GMOs in food and feed. Analytical and bioanalytical chemistry, 392(3), pp.369-384.
[7] Gryson, N., 2010. Effect of food processing on plant DNA degradation and PCR-based GMO analysis: a review. Analytical and Bioanalytical chemistry, 396(6), pp.2003-2022.
[8] Zhang, Q., Maroof, M.S., Lu, T.Y. and Shen, B.Z., 1992. Genetic diversity and differentiation of indica and japonica rice detected by RFLP analysis. Theoretical and Applied Genetics, 83(4), pp.495-499.
[9] Ahmed, F.E., 2002. Detection of genetically modified organisms in foods. TRENDS in Biotechnology, 20(5), pp.215-223.
[10] Vráblík, A., Hodek, J., Demnerová, K., Soukup, J. and Ovesná, J., 2012. Development and verification of PCR based assay to dectect and quantify garden pea lec gene. Czech Journal of Food Sciences, 30(3), pp.247-257.
[11] Safaei, P., Rezaie, S., Alimohammadi, M., Agha Kuchak Afshari, S., Mehdizadeh, M. and Molaee Aghaee, E., 2020. Qualitative PCR-based detection of genetically modified soy and maize products in Iran. International Journal of Food Properties, 23(1), pp.459-469.
[12] Ashrafi-Dehkordi E, Maghsoudi K, Babajafari S, Mazloomi M. Investigation on transgenic rice status in Shiraz consumer market using PCR. Genetic Engineering and Biosafety Journal. 2020; 9 (2) :204-212
[13] Ashrafi-Dehkordi, E., Mazloomi, S.M. and Hemmati, F., 2021. A comparison of DNA extraction methods and PCR-based detection of GMO in textured soy protein. Journal of Consumer Protection and Food Safety, 16(1), pp.51-57.
[14] Joelma, L.B., Olvia, C.P., Amlia, M.M., Artimsia, M.M., Lucinda, D.A., Lus, D.N. and Dcia, C., 2022. Detection of genetically modified organisms in food products commercialized in Mozambique. African Journal of Food Science, 16(2), pp.22-29.
[15] Rabiei, M., Mehdizadeh, M., Rastegar, H., Vahidi, H. and Alebouyeh, M., 2013. Detection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR. Iranian journal of pharmaceutical research: IJPR, 12(1), p.25.
[16] Brara, Z., Costa, J., Villa, C., Grazina, L., Bitam, A. and Mafra, I., 2020. Surveying genetically modified maize in foods marketed in Algeria. Food Control, 109, p.106928.
[17] Ashrafi-Dehkordi, E., Mazloomi, S.M. and Hemmati, F., 2021. A comparison of DNA extraction methods and PCR-based detection of GMO in textured soy protein. Journal of Consumer Protection and Food Safety, 16(1), pp.51-57.
[18] Cottenet, G., Blancpain, C., Sonnard, V. and Chuah, P.F., 2019. Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food. Food chemistry, 274, pp.760-765.