بررسی روش‌های ماسیراسیون و اولتراسوند بر ویژگی‌های آنتی‌اکسیدانی، آنتی‌آلزایمری و ضدمیکروبی عصاره جلبک پادینا (Padina distromatic)

نویسندگان
خدیجه شیرانی بیدآبادی 1
شیلا صفائیان 2
رضوان موسوی ندوشن 2
ناهید رحیمی فرد 3
1 دانشجوی دکترای تخصصی، گروه علوم و صنایع غذایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
2 دانشیار گروه علوم و صنایع غذایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
3 استاد تمام گروه میکروبیولوژی-اداره کل آزمایشگاه های کنترل غذا و دارو، سازمان غذا و دارو، وزارت بهداشت، درمان و آموزش پزشکی، تهران ایران
10.52547/fsct.19.122.199
چکیده
متابولیت‌های ثانویه جلبک‌های قهوه‌ای به دلیل فعالیت‌های بیولوژیکی مختلف از اهمیت ویژه‌ای برخوردارند. در این پژوهش هدف بررسی دو روش استخراج ماسیراسیون و اولتراسوند جهت تهیه عصاره از جلبک پادینا و ارزیابی عملکرد و کارایی روش‏های استخراج بر خواص آنتی‏اکسیدانی، فعالیت آنزیمی و ضدمیکروبی عصاره‏های تولیدی بود. عصاره جلبک پادینای تهیه شده با روش اولتراسوند از محتوای فنول (45/43 میلی‏گرم گالیک اسید در گرم) و فلاونوئید کل (59/24 میلی‏گرم کوئستین در هر گرم) بیشتری برخوردار بود. این در حالی است که حداکثر مهار رادیکال‏های آزاد در عصاره جلبک تهیه شده با روش ماسیراسیون مشاهده شد. عصاره جلبک پادینای تهیه شده با روش اولتراسوند بالاترین فعالیت بازدارندگی استیل کولین استراز را داشت و قوی‏ترین مهار تولید اکسید نیتریک در این عصاره مشاهده شد. باکتری‏های اشریشیا کلی و لیستریا اینوکوا ز بقیه میکروارگانیسم‏ها در برابر عصاره جلبک پادینا مقاوم‏تر بودند و نتایج آزمون میکروبی نشان داد عصاره جلبک پادینای تهیه شده به روش اولتراسوند در مقایسه با روش ماسیراسیون خاصیت ضدمیکروبی بیشتری داشت. در مجموع، نتایج به‌دست‌آمده شواهد ارزشمندی برای فعالیت آنتی‌اکسیدانی، آنتی‏آلزایمری و ضدمیکروبی ناشی از جلبک پادینا ارائه می‌دهد که می‌تواند رویکرد احتمالی آن را در استفاده درمانی برجسته کند.
کلیدواژه‌ها
آنتی‌اکسیدان
آنتی آلزایمر
جلبک قهوه‌ای
عصاره
فراصوت

موضوعات

عنوان مقاله English

Evaluation of different extraction methods (maceration and ultrasound) on antioxidant, anti-Alzheimer's and antimicrobial properties of Padina distromatic extract

نویسندگان English

khadijeh shirani bidabadi 1
shilla safaeian 2
rezvan mousavi nadushan 2
nahid rahimifard 3
1 epartment of Food Science and Technology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
2 epartment of Food Science and Technology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
3 Food and Drug Control Laboratories, Ministry of Health and Medical Education, Tehran, Iran.
چکیده English

Promising secondary metabolites of brown algae have been given particular importance, due to their various biological activities. In the present study, two extraction methods including maceration and ultrasound were performed to prepare extracts from brown algae including Padina distromatic, then, aimed to evaluate antioxidant, antimicrobial, and anti-Alzheimer’s activities of extracts. Padina algae extract prepared by ultrasound method had higher total phenol (43.25 mg GAE/g) and flavonoid content (24.59 mg QE/gDA). However, the maximum antioxidant activity was observed in algae extract prepared by maceration method. Padina algae extract prepared by ultrasound method had the highest inhibitory activity of acetylcholinesterase and the strongest inhibition of nitric oxide production was observed in this extract. Escherichia coli and Listeria innocua bacteria were more resistant to Padina algae extract than other microorganisms and the results of microbial test showed that Padina algae extract prepared by ultrasound method was more antimicrobial than maceration method. Collectively, the obtained results provide valuable evidence for antioxidant, anti-Alzheimer, and antimicrobial activity driven by Padina distromatic extracts which can highlight their possible approach in the therapeutic utilization.

کلیدواژه‌ها English

Antioxidant
Anti-Alzheimer
Brown marine algae
Extract
Ultrasound
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