[1] Sultana, F., Wahab, M. A., Nahiduzzaman, M., Mohiuddin, M., Iqbal, M. Z., Shakil, A., ... & Asaduzzaman, M. (2023). Seaweed farming for food and nutritional security, climate change mitigation and adaptation, and women empowerment: A review. Aquaculture and Fisheries, 8(5), 463-480.
[2] Thao My, P. L., Sung, V. V., Dat, T. D., Nam, H. M., Phong, M. T., & Hieu, N. H. (2020). Ultrasound‐assisted extraction of fucoidan from Vietnamese brown seaweed Sargassum mcclurei and testing bioactivities of the extract. ChemistrySelect, 5(14), 4371-4380.
[3] Lomartire, S., & Gonçalves, A. M. (2022). An overview of potential seaweed-derived bioactive compounds for pharmaceutical applications. Marine Drugs, 20(2), 141.
[4] Konstantin, B., Anastasia, P., Nikolay, I., & Daria, P. (2023). Seasonal variations in the chemical composition of Arctic brown macroalgae. Algal Research, 72, 103112.
[5] Flores-Contreras, E. A., Araújo, R. G., Rodríguez-Aguayo, A. A., Guzmán-Román, M., García-Venegas, J. C., Nájera-Martínez, E. F., ... & Parra-Saldivar, R. (2023). Polysaccharides from the Sargassum and brown algae genus: extraction, purification, and their potential therapeutic applications. Plants, 12(13), 2445.
[6] Liu, J., Wu, S. Y., Chen, L., Li, Q. J., Shen, Y. Z., Jin, L., ... & Tong, H. B. (2020). Different extraction methods bring about distinct physicochemical properties and antioxidant activities of Sargassum fusiforme fucoidans. International journal of biological macromolecules, 155, 1385-1392.
[7] Zhao, D., Xu, J., & Xu, X. (2018). Bioactivity of fucoidan extracted from Laminaria japonica using a novel procedure with high yield. Food chemistry, 245, 911-918.
[8] January, G. G., Naidoo, R. K., Kirby-McCullough, B., & Bauer, R. (2019). Assessing methodologies for fucoidan extraction from South African brown algae. Algal Res 40, 101517.
[9] Alboofetileh, M., Rezaei, M., & Tabarsa, M. (2019). Enzyme-assisted extraction of Nizamuddinia zanardinii for the recovery of sulfated polysaccharides with anticancer and immune-enhancing activities. Journal of Applied Phycology, 31(2), 1391-1402.
[10] Bordoloi, A., & Goosen, N. J. (2020). A greener alternative using subcritical water extraction to valorize the brown macroalgae Ecklonia maxima for bioactive compounds. Journal of Applied Phycology, 32(4), 2307-2319.
[11] Hans, N., Pattnaik, F., Malik, A., & Naik, S. (2023). Comparison of different green extraction techniques and their influence on chemical characteristics of sulfated polysaccharide (fucoidan) from Padina tetrastromatica and Turbinaria conoides. Algal Research, 74, 103199.
[12] Zayed, A., Finkelmeier, D., Hahn, T., Rebers, L., Shanmugam, A., Burger-Kentischer, A., & Ulber, R. (2023). Characterization and cytotoxic activity of microwave-assisted extracted crude fucoidans from different brown seaweeds. Marine drugs, 21(1), 48.
[13] Mabate, B., Daub, C. D., Pletschke, B. I., & Edkins, A. L. (2023). Comparative analyses of fucoidans from South African brown seaweeds that inhibit adhesion, migration, and long-term survival of colorectal cancer cells. Marine Drugs, 21(4), 203.
[14] Saravana, P. S., Cho, Y. J., Park, Y. B., Woo, H. C., & Chun, B. S. (2016). Structural, antioxidant, and emulsifying activities of fucoidan from Saccharina japonica using pressurized liquid extraction. Carbohydrate Polymers, 153, 518-525.
[15] Lee, S. H., Ko, C. I., Ahn, G., You, S., Kim, J. S., Heu, M. S., ... & Jeon, Y. J. (2012). Molecular characteristics and anti-inflammatory activity of the fucoidan extracted from Ecklonia cava. Carbohydrate polymers, 89(2), 599-606.
[16] Fitton, J. H., Stringer, D. N., & Karpiniec, S. S. (2015). Therapies from fucoidan: An update. Marine drugs, 13(9), 5920-5946.
[17] Cikoš, A. M., Jokić, S., Šubarić, D., & Jerković, I. (2018). Overview on the application of modern methods for the extraction of bioactive compounds from marine macroalgae. Marine drugs, 16(10), 348.
[18] Florez-Fernandez, N., Torres, M. D., González-Muñoz, M. J., & Dominguez, H. (2018). Potential of intensification techniques for the extraction and depolymerization of fucoidan. Algal research, 30, 128-148.
[19] Nguyen, A. N., Van Ngo, Q., Quach, T. T. M., Ueda, S., Yuguchi, Y., Matsumoto, Y., ... & Thanh, T. T. T. (2024). Fucoidan from brown seaweed Tubinaria decurrens: Structure and structure-anticancer activity relationship. International Journal of Biological Macromolecules, 259, 129326.
[20] Lorbeer, A. J., Lahnstein, J., Fincher, G. B., Su, P., & Zhang, W. (2015). Kinetics of conventional and microwave-assisted fucoidan extractions from the brown alga, Ecklonia radiata. Journal of Applied Phycology, 27(5), 2079-2087.
[21] Zayed, A., & Ulber, R. (2020). Fucoidans: Downstream processes and recent applications. Marine drugs, 18(3), 170.
[22] Fauziee, N. A. M., Chang, L. S., Mustapha, W. A. W., Nor, A. R. M., & Lim, S. J. (2021). Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana). International journal of biological macromolecules, 167, 1135-1145.
[23] Laeliocattleya, R. A., Yunianta, Y., Risjani, Y., & Wulan, S. N. (2025). Characterization of ‘novel fucoidan’extracted from brown seaweed (Sargassum echinocarpum J. Ag.) using Ultrasound-Assisted Extraction (UAE) and its potential antioxidant activity. Natural Product Research, 39(7), 1889-1901.
[24] Wang, S. H., Huang, C. Y., Chen, C. Y., Chang, C. C., Huang, C. Y., Dong, C. D., & Chang, J. S. (2021). Isolation and purification of brown algae fucoidan from Sargassum siliquosum and the analysis of anti-lipogenesis activity. Biochemical Engineering Journal, 165, 107798.
[25] Suprunchuk, V. (2021). Ultrasonic-treated fucoidan as a promising therapeutic agent. Polymers in Medicine, 51(2), 85-90.
[26] Flórez-Fernández, N., López-García, M., González-Muñoz, M. J., Vilariño, J. M. L., & Domínguez, H. (2017). Ultrasound-assisted extraction of fucoidan from Sargassum muticum. Journal of Applied Phycology, 29(3), 1553-1561.
[27] Chemat, F., Rombaut, N., Sicaire, A. G., Meullemiestre, A., Fabiano-Tixier, A. S., & Abert-Vian, M. (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics sonochemistry, 34, 540-560
[28] Lavilla, I., & Bendicho, C. (2017). Fundamentals of ultrasound-assisted extraction. In Water extraction of bioactive compounds, 291-316.
[29] Tecson, M. G., Abad, L. V., Ebajo Jr, V. D., & Camacho, D. H. (2021). Ultrasound-assisted depolymerization of kappa-carrageenan and characterization of degradation product. Ultrasonics Sonochemistry, 73, 105540.
[30] Ramu, S., Murali, A., Narasimhaiah, G., & Jayaraman, A. (2020). Toxicological evaluation of Sargassum Wightii greville derived fucoidan in wistar rats: Haematological, biochemical and histopathological evidences. Toxicology Reports, 7, 874-882.
[31] Dwijayanto, A., Al-Yousefi, A. M. A. A., Puspasari, I., & Markom, M. (2025). Comparative Study on the Extraction of Crude Fucoidan from Brown Seaweed using Conventional, Microwave and Ultrasound-Assisted Methods. AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment), 9(1), 1-6.
[32] Antonisamy, A. J., & Rajendran, K. (2024). Comparative study on the extraction methods, characterization, and bioactivity of crude fucoidan, a polysaccharide derived from Sargassum ilicifolium. Biochemical Engineering Journal, 209, 109398.
[33] Dobrincic, A., Pedisic, S., Zoric, Z., Jurin, M., Roje, M., Coz-Rakovac, R., & Dragovic-Uzelac, V. (2021). Microwave assisted extraction and pressurized liquid extraction of sulfated polysaccharides from Fucus virsoides and Cystoseira barbata. Foods, 10, 1481.
[34] Amarante, S. J., Catarino, M. D., Marçal, C., Silva, A. M., Ferreira, R., & Cardoso, S. M. (2020). Microwave-assisted extraction of phlorotannins from Fucus vesiculosus. Marine drugs, 18(11), 559.
[35] Cui, R., & Zhu, F. (2021). Ultrasound modified polysaccharides: A review of structure, physicochemical properties, biological activities and food applications. Trends in Food Science & Technology, 107, 491-508.
[36] Quitério, E., Grosso, C., Ferraz, R., Delerue-Matos, C., & Soares, C. (2022). A critical comparison of the advanced extraction techniques applied to obtain health-promoting compounds from seaweeds. Marine drugs, 20(11), 677.
[37] Liu, Y., Liu, X., Cui, Y., & Yuan, W. (2022). Ultrasound for microalgal cell disruption and product extraction: A review. Ultrasonics Sonochemistry, 87, 106054.
[38] Sasaki, C., Tamura, S., Suzuki, M., Etomi, K., Nii, N., Hayashi, J., & Kanemaru, K. (2024). Continuous microwave-assisted step-by-step extraction of bioactive water-soluble materials and fucoidan from brown seaweed Undaria pinnatifida waste. Biomass Conversion and Biorefinery, 14(6), 7673-7682.
[39] Franca-Oliveira, G., Fornari, T., & Hernández-Ledesma, B. (2021). A review on the extraction and processing of natural source-derived proteins through eco-innovative approaches. Processes, 9(9), 1626.
[40] Zayed, A., Finkelmeier, D., Hahn, T., Rebers, L., Shanmugam, A., Burger-Kentischer, A., & Ulber, R. (2023). Characterization and cytotoxic activity of microwave-assisted extracted crude fucoidans from different brown seaweeds. Marine drugs, 21(1), 48.
[41] Magnusson, M., Glasson, C. R. K., Vucko, M. J., Angell, A., Neoh, T. L., & de Nys, R. (2019). Enrichment processes for the production of high-protein feed from the green seaweed Ulva ohnoi. Algal Res 41, 101555.
[42] Quitain, A. T., Kai, T., Sasaki, M., & Goto, M. (2013). Microwave–hydrothermal extraction and degradation of fucoidan from supercritical carbon dioxide deoiled Undaria pinnatifida. Industrial & engineering chemistry research, 52(23), 7940-7946.
[43] Wang, S. H., Huang, C. Y., Chen, C. Y., Chang, C. C., Huang, C. Y., Dong, C. D., & Chang, J. S. (2021). Isolation and purification of brown algae fucoidan from Sargassum siliquosum and the analysis of anti-lipogenesis activity. Biochemical Engineering Journal, 165, 107798.
[44] Wu, S. C. (2017). Antioxidant activity of sulfated seaweeds polysaccharides by novel assisted extraction. Solubility of Polysaccharides; Xu, Z., Ed.; IntechOpen: London, UK, 89-108.
[45] Balboa, E. M., Moure, A., & Domínguez, H. (2015). Valorization of Sargassum muticum biomass according to the biorefinery concept. Marine Drugs, 13(6), 3745-3760.
[46] Rizkiyah, D. N., Putra, N. R., Idham, Z., Che Yunus, M. A., Veza, I., Harny, I., ... & Abdul Aziz, A. H. (2022). Optimization of red pigment anthocyanin recovery from Hibiscus sabdariffa by subcritical water extraction. Processes, 10(12), 2635.
[47] Saravana, P. S., Cho, Y. J., Park, Y. B., Woo, H. C., & Chun, B. S. (2016). Structural, antioxidant, and emulsifying activities of fucoidan from Saccharina japonica using pressurized liquid extraction. Carbohydrate Polymers, 153, 518-525.
[48] Chatzimitakos, T., Athanasiadis, V., Kotsou, K., Mantiniotou, M., Kalompatsios, D., Makrygiannis, I., ... & Lalas, S. I. (2024). Optimization of pressurized liquid extraction (PLE) parameters for extraction of bioactive compounds from Moringa oleifera leaves and bioactivity assessment. International Journal of Molecular Sciences, 25(9), 4628.
[49] Fertah, M. (2017). Isolation and Characterization of Alginate from Seaweed. Seaweed Polysaccharides, 11–26.
[50] Mapholi, Z., & Goosen, N. J. (2023). Optimization of fucoidan recovery by ultrasound-assisted enzymatic extraction from South African kelp, Ecklonia maxima. Ultrasonics Sonochemistry, 101, 106710.
[51] Tang, S., Ma, Y., Dong, X., Zhou, H., He, Y., Ren, D., ... & Wu, L. (2022). Enzyme-assisted extraction of fucoidan from Kjellmaniella crassifolia based on kinetic study of enzymatic hydrolysis of algal cellulose. Algal Research, 66, 102795.
[52] Zainal-Abidin, M. H., Hayyan, M., Hayyan, A., & Jayakumar, N. S. (2017). New horizons in the extraction of bioactive compounds using deep eutectic solvents: A review. Analytica chimica acta, 979, 1-23.
[53] Irianto, I., Naryaningsih, A., Trisnawati, N. W., Astuti, A., Komariyah, K., Qomariyah, L., ... & Putra, N. R. (2024). From sea to solution: A review of green extraction approaches for unlocking the potential of brown algae. South African Journal of Chemical Engineering, 48(1), 1-21.
[54] Cunha, S. C., & Fernandes, J. O. (2018). Extraction techniques with deep eutectic solvents. TrAC Trends in Analytical Chemistry, 105, 225-239.
[55] Saravana, P. S., Cho, Y. N., Woo, H. C., & Chun, B. S. (2018). Green and efficient extraction of polysaccharides from brown seaweed by adding deep eutectic solvent in subcritical water hydrolysis. Journal of Cleaner Production, 198, 1474-1484.
[56] James, K., Ramirez, J., Barner, L., & Moghaddam, L. (2024). Technology comparison for sequential extraction of fucoidan and sodium alginate from Ascophyllum nodosum using a glycerol and choline chloride solvent. Cleaner Engineering and Technology, 18, 100707.
[57] Choi, Y. Y., Lee, S. J., Lee, Y. J., Kim, H. S., Eom, J. S., Kim, S. C., ... & Lee, S. S. (2020). New challenges for efficient usage of Sargassum fusiforme for ruminant production. Scientific reports, 10(1), 19655.
[58] Lähteenmäki-Uutela, A., Rahikainen, M., Camarena-Gómez, M. T., Piiparinen, J., Spilling, K., & Yang, B. (2021). European Union legislation on macroalgae products. Aquaculture International, 29(2), 487-509.
[59] Wells, M. L., Potin, P., Craigie, J. S., Raven, J. A., Merchant, S. S., Helliwell, K. E., ... & Brawley, S. H. (2017). Algae as nutritional and functional food sources: revisiting our understanding. Journal of applied phycology, 29(2), 949-982.
[60] Potoroko, I. Y., Uskova, D. G., Pajmulina, A. V., & Uday, B. (2019). Ultrasound micronization of fucoidan vegetable ingredient for the use in food production technology. Bull. South Ural. Univ. Ser. Food Biotechnol, 7, 58-70.
[61] Gullón, P., Astray, G., Gullón, B., Franco, D., Campagnol, P. C. B., & Lorenzo, J. M. (2021). Inclusion of seaweeds as healthy approach to formulate new low-salt meat products. Current Opinion in Food Science, 40, 20-25.
[62] Lamont, T., & McSweeney, M. (2021). Consumer acceptability and chemical composition of whole‐wheat breads incorporated with brown seaweed (Ascophyllum nodosum) or red seaweed (Chondrus crispus). Journal of the Science of Food and Agriculture, 101(4), 1507-1514.
[63] Poveda-Castillo, G. D. C., Rodrigo, D., Martínez, A., & Pina-Pérez, M. C. (2018). Bioactivity of fucoidan as an antimicrobial agent in a new functional beverage. Beverages, 4(3), 64.
[64] Koh, H. S. A., Lim, S. E. V., Lu, J., & Zhou, W. (2020). Bioactivity enhancement of fucoidan through complexing with bread matrix and baking. LWT, 130, 109646.
[65] Simonazzi, A., Cid, A. G., Villegas, M., Romero, A. I., Palma, S. D., & Bermúdez, J. M. (2018). Nanotechnology applications in drug controlled release. Drug Targeting and Stimuli Sensitive Drug Delivery Systems, 81–116.
[66] Mwangi, W. W., Lim, H. P., Low, L. E., Tey, B. T., & Chan, E. S. (2020). Food-grade Pickering emulsions for encapsulation and delivery of bioactives. Trends in Food Science & Technology, 100, 320-332.
[67] Oliyaei, N., Moosavi-Nasab, M., & Tanideh, N. (2022). Preparation of Fucoxanthin Nanoemulsion Stabilized by Natural Emulsifiers: Fucoidan, Sodium Caseinate, and Gum Arabic. Molecules, 27(19), 6713.
[68] Jayakody, M. M., Kaushani, K. G., & Vanniarachchy, M. P. G. (2022). Edible Seaweed-Based Biodegradable Films and Coatings for Food and Nutraceutical Applications. Algal Functional Foods and Nutraceuticals: Benefits, Opportunities, and Challenges, 429–446.
[69] Lan, Y., Liu, Y., Li, X., & Wu, S. (2024). Fucoidan-based coatings extend the shelf-life of nectarines. Food Chemistry: X, 22, 101479.
[70] Wang, F., Xie, C., Tang, H., Li, H., Hou, J., Zhang, R., ... & Jiang, L. (2023). Intelligent packaging based on chitosan/fucoidan incorporated with coleus grass (Plectranthus scutellarioides) leaves anthocyanins and its application in monitoring the spoilage of salmon (Salmo salar L.). International Journal of Biological Macromolecules, 252, 126423.
[71] Malekkolaei, K. J., Jafarian, S., Javadian, S. R., Mahdavi, S. K., & Bahram, S. (2025). Edible coatings of chitosan and fucoidan with crucian carp protein hydrolysate to extend shelf life of beluga sturgeon fillets. Heliyon, 11(4), 42296.
[72] Xu, B., & Wu, S. (2021). Preservation of mango fruit quality using fucoidan coatings. Lwt, 143, 111150.
[73] Luo, A., Feng, J., Hu, B., Lv, J., Liu, Q., Nan, F., ... & Xie, S. (2018). Arthrospira (Spirulina) platensis extract improves oxidative stability and product quality of Chinese-style pork sausage. Journal of Applied Phycology, 30(3), 1667-1677.
[74] Luo, P., Li, F., Liu, H., Yang, X., & Duan, Z. (2020). Effect of fucoidan‐based edible coating on antioxidant degradation kinetics in strawberry fruit during cold storage. Journal of Food Processing and Preservation, 44(4), 14381.
[75] Irianto, I., Naryaningsih, A., Trisnawati, N. W., Astuti, A., Komariyah, K., Qomariyah, L., ... & Putra, N. R. (2024). From sea to solution: A review of green extraction approaches for unlocking the potential of brown algae. South African Journal of Chemical Engineering, 48(1), 1-21.
[76] Mensah, E. O., Kanwugu, O. N., Panda, P. K., & Adadi, P. (2023). Marine fucoidans: Structural, extraction, biological activities and their applications in the food industry. Food Hydrocolloids, 142, 1-25.
[77] Nguyen, T. T., Mikkelsen, M. D., Tran, V. H. N., Trang, V. T. D., Rhein-Knudsen, N., Holck, J., ... & Meyer, A. S. (2020). Enzyme-assisted fucoidan extraction from brown macroalgae Fucus distichus subsp. evanescens and Saccharina latissima. Marine Drugs, 18(6), 296.
[78] James, K., Long, H., O'Hara, I., Williams, B., & Moghaddam, L. (2024). Bio-based film development: Harnessing alginate and fucoidan extracted from Ascophyllum nodosum with glycerol and choline chloride-based solvent. Food Hydrocolloids, 153, 110013.
[79] Rhein-Knudsen, N., Reyes-Weiss, D., & Horn, S. J. (2023). Extraction of high purity fucoidans from brown seaweeds using cellulases and alginate lyases. International Journal of Biological Macromolecules, 229, 199-209.
[80] Alboofetileh, M., Rezaei, M., Tabarsa, M., Rittà, M., Donalisio, M., Mariatti, F., ... & Cravotto, G. (2019). Effect of different non-conventional extraction methods on the antibacterial and antiviral activity of fucoidans extracted from Nizamuddinia zanardinii. International journal of biological macromolecules, 124, 131-137.
[81] Dobrincic, A., Pedisic, S., Zoric, Z., Jurin, M., Roje, M., Coz-Rakovac, R., & Dragovic-Uzelac, V. (2021). Microwave assisted extraction and pressurized liquid extraction of sulfated polysaccharides from Fucus virsoides and Cystoseira barbata. Foods, 10, 1481.
[82] Kadam, S. U., Tiwari, B. K., Smyth, T. J., & O’Donnell, C. P. (2015). Optimization of ultrasound assisted extraction of bioactive components from brown seaweed Ascophyllum nodosum using response surface methodology. Ultrasonics Sonochemistry, 23, 308-316.
[83] Lin, P., Chen, S., Liao, M., & Wang, W. (2022). Physicochemical characterization of fucoidans from Sargassum henslowianum C. Agardh and their antithrombotic activity in vitro. Marine Drugs, 20(5), 300.
[84] López-Salazar, H., Camacho-Díaz, B. H., Ocampo, M. A., & Jiménez-Aparicio, A. R. (2023). Microwave-assisted extraction of functional compounds from plants: A Review. BioResources, 18(3), 6614.
[85] Cannavacciuolo, C., Pagliari, S., Celano, R., Campone, L., & Rastrelli, L. (2024). Critical analysis of green extraction techniques used for botanicals: Trends, priorities, and optimization strategies-A review. TrAC Trends in Analytical Chemistry, 173, 117627.
[86] Geow, C. H., Tan, M. C., Yeap, S. P., & Chin, N. L. (2021). A review on extraction techniques and its future applications in industry. European Journal of Lipid Science and Technology, 123(4), 2000302.
[87] Ummat, V., Sivagnanam, S. P., Rai, D. K., O’Donnell, C., Conway, G. E., Heffernan, S. M., ... & Tiwari, B. K. (2024). Conventional extraction of fucoidan from Irish brown seaweed Fucus vesiculosus followed by ultrasound-assisted depolymerization. Scientific Reports, 14(1), 1–13.
