مجله علوم و صنایع غذایی ایران

مجله علوم و صنایع غذایی ایران

Dietary Supplementation with Moringa oleifera and Turmeric Synergistically Enhances Immuno-Antioxidant Status and Alleviates Heat Stress in Shami Goats

نوع مقاله : مقاله پژوهشی

نویسندگان
University of Tikrit, College of Education for Pure science, Department of life sciences
چکیده
The current study aimed at determining whether dietary supplementation with the Moringa oleifera leaf extract (MOLE) and turmeric would mitigate against the adverse effects of heat stress on immune functions and the oxidative status of Shami goats. Twenty-four adult female Shami goats Shami goats were randomly assigned four equal groups and exposed to a natural heat stress (THI: 82-92) over 60 days. The control group was served with the basal diet whereas the treatment groups were served with the basal diet supplemented with 3% MOLE, 2% turmeric and 1.5% MOLE plus 1% turmeric. Antioxidant enzymes, inflammatory cytokines, lymphocyte populations, immunoglobulins and heat shock proteins were measured by taking blood samples at biweekly intervals. The combined supplementation group had significant improvements on all the measured parameters. The antioxidant enzyme activities were higher and the SOD activity was higher (93.40 U/mL) compared to controls (68.25 U/mL). The pro inflammatory cytokines were reduced significantly -IL-6 reduced to 22.1 pg/mL and TNF-a reduced to 18.4 pg/mL. The proportion of CD4+/CD8+ increased to 1.8, which is a good sign of cellular immunity. The expression of heat shock protein 70 was 2.3-fold larger, whereas the concentration of IgG was raised by 12.3 to 16.8 mg/mL. These results indicate that Moringa and turmeric supplementation can be effective as a natural approach to beat heat stress in goats through improvement of antioxidant defenses, attenuation of inflammation and maintenance of immune functions.
کلیدواژه‌ها
موضوعات

[1]    Rashamol, V. P., Sejian, V., Bagath, M., Krishnan, G., Archana, P. R., & Bhatta, R. (2018). Physiological adaptability of livestock to heat stress: an updated review. Journal of Animal Behaviour and Biometeorology, 6(3), 62-71.‏
[2]    Younis, F. E., & Khidr, R. E. (2020). Mitigation and adaptation strategies for livestock to cope with climate change challenges: review and perspective. Animal Science Reporter, 13(4).‏
[3]    Lauridsen, C. (2019). From oxidative stress to inflammation: redox balance and immune system. Poultry science, 98(10), 4240-4246.‏
[4]    Kunst, C., Schmid, S., Michalski, M., Tümen, D., Buttenschön, J., Müller, M., & Gülow, K. (2023). The influence of gut microbiota on oxidative stress and the immune system. Biomedicines, 11(5), 1388.‏
[5]    Michaeloudes, C., Abubakar-Waziri, H., Lakhdar, R., Raby, K., Dixey, P., Adcock, I. M., ... & Chung, K. F. (2022). Molecular mechanisms of oxidative stress in asthma. Molecular aspects of medicine, 85, 101026.‏
[6]    Pareek, A., Pant, M., Gupta, M. M., Kashania, P., Ratan, Y., Jain, V., ... & Chuturgoon, A. A. (2023). Moringa oleifera: An updated comprehensive review of its pharmacological activities, ethnomedicinal, phytopharmaceutical formulation, clinical, phytochemical, and toxicological aspects. International journal of molecular sciences, 24(3), 2098.‏
[7]    Menon, V. P., & Sudheer, A. R. (2007). Antioxidant and anti-inflammatory properties of curcumin. The molecular targets and therapeutic uses of curcumin in health and disease, 105-125.‏
[8]    Ashrafizadeh, M., Ahmadi, Z., Mohammadinejad, R., Farkhondeh, T., & Samarghandian, S. (2020). Curcumin activates the Nrf2 pathway and induces cellular protection against oxidative injury. Current Molecular Medicine, 20(2), 116-133.‏
[9]    Vl, S. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.‏
[10] Marklund, S., & Marklund, G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European journal of biochemistry, 47(3), 469-474.‏
[11] Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121-126.
[12] Paglia, D. E., & Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of laboratory and clinical medicine, 70(1), 158-169.‏
[13] He, F., Ru, X., & Wen, T. (2020). NRF2, a transcription factor for stress response and beyond. International journal of molecular sciences, 21(13), 4777.‏
[14] Gambhir, L., Tyagi, G., Bhardwaj, R., Kapoor, N., & Sharma, G. (2022). Status: Role of Nrf2, a Master Regulator of Cellular Redox. Reactive oxygen species, 181.‏
[15] Trougakos, I. P. (2019). Nrf2, stress and aging. Aging (Albany NY), 11(15), 5289.‏
[16] Luckheeram, R. V., Zhou, R., Verma, A. D., & Xia, B. (2012). CD4+ T cells: differentiation and functions. Journal of Immunology Research, 2012(1), 925135.‏
[17] Takeuchi, A., & Saito, T. (2017). CD4 CTL, a cytotoxic subset of CD4+ T cells, their differentiation and function. Frontiers in immunology, 8, 194.‏
[18] Chauhan, S. S., Rashamol, V. P., Bagath, M., Sejian, V., & Dunshea, F. R. (2021). Impacts of heat stress on immune responses and oxidative stress in farm animals and nutritional strategies for amelioration. International journal of biometeorology, 65(7), 1231-1244.‏
[19] Archana, P. R., Aleena, J., Pragna, P., Vidya, M. K., Niyas, A. P. A., Bagath, M., ... & Bhatta, R. (2017). Role of heat shock proteins in livestock adaptation to heat stress. J. Dairy Vet. Anim. Res, 5(1), 00127.‏
[20] Sadiq, I. Z. (2023). Free radicals and oxidative stress: Signaling mechanisms, redox basis for human diseases, and cell cycle regulation. Current molecular medicine, 23(1), 13-35.‏
[21] Ribeiro, T. P., Fernandes, C., Melo, K. V., Ferreira, S. S., Lessa, J. A., Franco, R. W., ... & Horn Jr, A. (2015). Iron, copper, and manganese complexes with in vitro superoxide dismutase and/or catalase activities that keep Saccharomyces cerevisiae cells alive under severe oxidative stress. Free Radical Biology and Medicine, 80, 67-76.‏
[22] Wang, S., Meckling, K. A., Marcone, M. F., Kakuda, Y., & Tsao, R. (2011). Synergistic, additive, and antagonistic effects of food mixtures on total antioxidant capacities. Journal of agricultural and food chemistry, 59(3), 960-968.‏
[23] Olszowy-Tomczyk, M. (2020). Synergistic, antagonistic and additive antioxidant effects in the binary mixtures. Phytochemistry Reviews, 19(1), 63-103.‏
[24] Hajimehdipoor, H., Shahrestani, R., & Shekarchi, M. (2014). Investigating the synergistic antioxidant effects of some flavonoid and phenolic compounds. Research journal of pharmacognosy, 1(3), 35-40.‏
[25] Vicol, C., & Duca, G. (2023). Synergistic, Additive, Antagonistic Effects and the Prooxidant Character of Antioxidants: Interactions in Natural Compounds. In Fundamental and Biomedical Aspects of Redox Processes (pp. 224-249). IGI Global Scientific Publishing.‏
[26] Chisoro, P., Jaja, I. F., & Assan, N. (2023). Incorporation of local novel feed resources in livestock feed for sustainable food security and circular economy in Africa. Frontiers in Sustainability, 4, 1251179.‏
[27] Rodríguez, R., Scull, I., & Montejo, I. L. (2017). Nutritional value of Moringa oleifera (moringa) for animal feeding. Mulberry, moringa and thitonia in animal feed, and other uses. Results in Latin America and the Caribbean. San José de las Lajas, Cuba: FAO, EDICA, 125-140.‏
[28] Mahfuz, S., & Piao, X. S. (2019). Application of Moringa (Moringa oleifera) as natural feed supplement in poultry diets. Animals, 9(7), 431.‏
[29] Klein, S. L., & Flanagan, K. L. (2016). Sex differences in immune responses. Nature Reviews Immunology, 16(10), 626-638.‏
[30] Carroll, J. A., Burdick, N. C., Chase Jr, C. C., Coleman, S. W., & Spiers, D. E. (2012). Influence of environmental temperature on the physiological, endocrine, and immune responses in livestock exposed to a provocative immune challenge. Domestic Animal Endocrinology, 43(2), 146-153.‏
[31] Vesic, Z., Jakovljevic, V., Nikolic Turnic, T., Vukasinovic-Vesic, M., Bolevich, S., & Radakovic, S. (2021). The influence of acclimatization on stress hormone concentration in serum during heat stress. Molecular and Cellular Biochemistry, 476(9), 3229-3239.‏