Deep eutectic solvents have found many advantages in the extraction of plant metabolites, such as low toxicity, biodegradability, low cost, and ease of preparation compared to conventional methods. The aim of this work is to compare the natural deep eutectic solvents in the extraction of Ferula gummosa (Ferula gummosa) and determine its chemical, structural, thermal and rheological properties. Six eutectic solvent mixtures including choline chloride/urea, acetic acid, lactic acid, formic acid, formamide and glycerol in ratios of 2:1 and 3:1 were evaluated. The highest yield was obtained for choline chloride/formic acid, choline chloride/formamide. The main components of oleoresin were β-pinene (40.27%), silcophenone (11.93%) and alpha-pinene (7.53%), which was determined by gas chromatography-mass spectrometry. Gum was mainly composed of carbohydrates (67.39% by weight). The X-ray diffraction pattern showed a semi-crystalline microstructure. The thermal behavior of the gum was evaluated by thermal analysis (TGA) and differential scanning calorimetry (DSC) showed temperatures below and above 200 °C as the dominant weight loss regions. The rheological behavior of oleoresin was non-Newtonian behavior of shear thinning type, which was well fitted by the power law model. Studying the chemical structure by spectrometry showed that no solvent remained in oleoresin. Therefore, barijeh oleoresin can be used as a promising natural medicinal substance extracted with eutectic solvents, while these eutectic solvents can be used as a green method for many herbal compounds.