Effect of clove oil extract on the growth of microorganisms and physicochemical compounds in local meat

نویسندگان
1 دانشگاه جیهان، دهوک، عراق
2 دانشگاه فنی شمالی، موصل، عراق
چکیده
The purpose of this research is to analyze the impact of local clove oil extract on both the microbial growth and the physicochemical characteristic of locally produced meat. With increased awareness towards food safety and negative impacts of synthetic preservatives, the study looks at natural preservatives like Clove oil. The main purpose was to assess the efficiency of Clove oil extract in eliminating bacterial load particularly E.coli S. aureus L. monocytogenes Salmonella, Clostridium perfringens while studying the effect of incubation period on physicochemical parameters such as pH, moisture content, fat content, protein content, color and texture. Meat samples were prepared from the local markets and they were subjected to clove oil extract with different concentrations of 1% and 5%. These cultures were taken before the treatment, on the third and seventh days of lacto-fermentation, using methods such as one-way ANOVA and Tukey’s HSD to test for significance. As for the findings, the results clearly showed that the level of microbial reduction as well as the physicochemical qualities was enhanced by the treatment with clove oil especially at higher concentrations of the oil. The pH level has reduced, moisture content also reduced, protein content increased and there was also a reduction in lipid oxidation. Therefore, this study has effectively demonstrated that local meat could be preserved using clove oil extract, thereby improving its safety and quality. Therefore, it is possible to apply clove oil in the meat industry instead of synthetic additives to ensure food safety and benefit the consumers. Further studies should be conducted to investigate the effects of this flavor on the sense organs and other uses in different types of meat products.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Effect of clove oil extract on the growth of microorganisms and physicochemical compounds in local meat

نویسندگان English

Khalida Hasan 1
Yasir Ameen 1
Maha Al-Jawadi 2
1 Cihan University, Duhok, Iraq
2 Northern Technical University, Mosul, Iraq
چکیده English

The purpose of this research is to analyze the impact of local clove oil extract on both the microbial growth and the physicochemical characteristic of locally produced meat. With increased awareness towards food safety and negative impacts of synthetic preservatives, the study looks at natural preservatives like Clove oil. The main purpose was to assess the efficiency of Clove oil extract in eliminating bacterial load particularly E.coli S. aureus L. monocytogenes Salmonella, Clostridium perfringens while studying the effect of incubation period on physicochemical parameters such as pH, moisture content, fat content, protein content, color and texture. Meat samples were prepared from the local markets and they were subjected to clove oil extract with different concentrations of 1% and 5%. These cultures were taken before the treatment, on the third and seventh days of lacto-fermentation, using methods such as one-way ANOVA and Tukey’s HSD to test for significance. As for the findings, the results clearly showed that the level of microbial reduction as well as the physicochemical qualities was enhanced by the treatment with clove oil especially at higher concentrations of the oil. The pH level has reduced, moisture content also reduced, protein content increased and there was also a reduction in lipid oxidation. Therefore, this study has effectively demonstrated that local meat could be preserved using clove oil extract, thereby improving its safety and quality. Therefore, it is possible to apply clove oil in the meat industry instead of synthetic additives to ensure food safety and benefit the consumers. Further studies should be conducted to investigate the effects of this flavor on the sense organs and other uses in different types of meat products.

کلیدواژه‌ها English

Clove oil extract
Physicochemical properties
Lipid oxidation
Antimicrobial activity
Essential oils
1. Kumar Pandey, V., Shams, R., Singh, R., Dar, A. H., Pandiselvam, R., Rusu, A. V., & Trif, M. (2022). A comprehensive review on clove (Caryophyllus aromaticus L.) essential oil and its significance in the formulation of edible coatings for potential food applications. Frontiers in nutrition, 9, 987674.
2. Bisholo, K. Z., Ghuman, S., & Haffejee, F. (2018). Food-borne disease prevalence in rural villages in the Eastern Cape, South Africa. African Journal of Primary Health Care and Family Medicine, 10(1), 1-5.‏
3. Costa, K. A. D., Moura, R., & Millezi, A. F. (2019). Antimicrobial and antibiofilm activity of Cymbopogon flexuosus essential oil microemulsions. Revista Ceres, 66, 372-379.‏
4. Nuñez, L., & D'Aquino, M. (2012). Microbicide activity of clove essential oil (Eugenia caryophyllata). Brazilian journal of microbiology, 43, 1255-1260.‏
5. Delgado-Adámez, J., Fernández-León, M. F., Velardo-Micharet, B., & González-Gómez, D. (2012). In vitro assays of the antibacterial and antioxidant activity of aqueous leaf extracts from different Prunus salicina Lindl. cultivars. Food and chemical toxicology, 50(7), 2481-2486.‏
6. Bajpai, V. K., Baek, K. H., & Kang, S. C. (2012). Control of Salmonella in foods by using essential oils: A review. Food Research International, 45(2), 722-734.
7. ‏ Plati, F., & Paraskevopoulou, A. (2022). Micro-and nano-encapsulation as tools for essential oils advantages’ exploitation in food applications: The case of oregano essential oil. Food and bioprocess technology, 15(5), 949-977.‏
8. Iqbal, R., Dahlawi, S. M., Mehmood, Z., & Khalid, N. (2022). Antimicrobial effectiveness of clove oil nanoemulsions against Escherichia coli O157: H7 and Staphylococcus aureus in fresh beef samples. Pakistan Journal of Agricultural Sciences, 59(4).‏
9. Yoo, J. H., Baek, K. H., Heo, Y. S., Yong, H. I., & Jo, C. (2021). Synergistic bactericidal effect of clove oil and encapsulated atmospheric pressure plasma against Escherichia coli O157: H7 and Staphylococcus aureus and its mechanism of action. Food Microbiology, 93, 103611.‏
10. Khaleque, M. A., Keya, C. A., Hasan, K. N., Hoque, M. M., Inatsu, Y., & Bari, M. L. (2016). Use of cloves and cinnamon essential oil to inactivate Listeria monocytogenes in ground beef at freezing and refrigeration temperatures. LWT, 74, 219-223.‏
11. Ji, J., Shankar, S., Royon, F., Salmieri, S., & Lacroix, M. (2023). Essential oils as natural antimicrobials applied in meat and meat products—A review. Critical Reviews in Food Science and Nutrition, 63(8), 993-1009.‏
12. Gómez, I., Janardhanan, R., Ibañez, F. C., & Beriain, M. J. (2020). The effects of processing and preservation technologies on meat quality: Sensory and nutritional aspects. Foods, 9(10), 1416.‏
13. Ricardo-Rodrigues, S., Rouxinol, M. I., Agulheiro-Santos, A. C., Potes, M. E., Laranjo, M., & Elias, M. (2024). The Antioxidant and Antibacterial Potential of Thyme and Clove Essential Oils for Meat Preservation—An Overview. Applied Biosciences, 3(1), 87-101.
14. ‏Ruiz-Hernández, K., Sosa-Morales, M. E., Cerón-García, A., & Gómez-Salazar, J. A. (2023). Physical, chemical and sensory changes in meat and meat products induced by the addition of essential oils: A concise review. Food Reviews International, 39(4), 2027-2056.‏
15. Nehme, R., Andrés, S., Pereira, R. B., Ben Jemaa, M., Bouhallab, S., Ceciliani, F., ... & Abdennebi-Najar, L. (2021). Essential oils in livestock: From health to food quality. Antioxidants, 10(2), 330.‏
16. Al-Jawadi, M. A., & Ahmed, S. O. (2024, July). Study of the Effect of Storage Period on the Growth of Fungi in Green Coffee in Open Atmospheres. In IOP Conference Series: Earth and Environmental Science (Vol. 1371, No. 6, p. 062015). IOP Publishing.‏
17. Ansarian, E., Aminzare, M., Azar, H. H., Mehrasbi, M. R., & Bimakr, M. (2022). Nanoemulsion-based basil seed gum edible film containing resveratrol and clove essential oil: In vitro antioxidant properties and its effect on oxidative stability and sensory characteristic of camel meat during refrigeration storage. Meat science, 185, 108716.‏
18. Zhang, H., Peng, X., Li, X., Wu, J., & Guo, X. (2017). The application of clove extract protects Chinese-style sausages against oxidation and quality deterioration. Korean Journal for Food Science of Animal Resources, 37(1), 114.
19. Pinto, L. A. D. M., Razente, R. A., Benito, C. E., Gubert, L., Stefanello, L. R., Simões, E. P., ... & Fernandes, J. I. (2022). Clove essential oil (Syzygium aromaticum L.) as a natural preservative to improve the shelf‐life of chicken patties with different degrees of myopathy. Journal of Food Processing and Preservation, 46(11), e17037.
20. Jayasena, D. D., & Jo, C. (2014). Potential application of essential oils as natural antioxidants in meat and meat products: A review. Food reviews international, 30(1), 71-90.
21. Al-jawadi, M, A.M & Ahmad S.O. (2023). Effect of storage on the concentration of total phenolic compound in brazilian green coffee. Al-kut University College Journal. (special issue), 498-504.