بررسی فعالیت ضدقارچی اسانس بادرشبو (Dracocephalum moldavica) و برهمکنش آن با آنتی‌بیوتیک نیستاتین علیه تعدادی از سویه‌های قارچی

نویسندگان
میترا قدسی شیخ جان 1
علی فضل آرا 2
محمد حجتی 3
بهروز علیزاده بهبهانی 4
1 1- دانشجوی دکتری بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
2 2- استاد گروه بهداشت و مواد غذایی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
3 3- استاد، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
4 4- دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.22034/FSCT.21.156.226
چکیده
اگرچه در حال حاضر اغلب از نگهدارنده‎های ضد‌قارچی شیمیای در فراورده‌های غذایی مختلف استفاده می شود، ولی استفاده از این مواد به دلیل اثرهای مضر آن‌ها بر سلامت انسان و محیط زیست محدود شده است. درسال‌های اخیر پژوهشگران به دنبال جایگزین کردن این ترکیب‌های شیمیایی با مواد طبیعی و کم خطر می‌باشند. در این راستا استفاده از اسانس گیاهان دارویی به دلیل عوارض جانبی کمتر می‌تواند جایگزین مناسبی محسوب شود. لذا در پژوهش حاضر پس از تهیه گیاه بادرشبو از مزارع روستای گلمرز واقع در نزدیکی شهرستان ارومیه و خشک کردن آن، اسانس‌گیری از گیاه بادرشبو به وسیله دستگاه کلونجر انجام شد و اثر ضدقارچی اسانس بادرشبو بر تعدادی از سویه‌های مهم قارچی با روش‌های انتشار در دیسک، انتشار در چاهک، حداقل غلظت بازدارندگی، حداقل غلظت کشندگی و بر‌همکنش اسانس بادرشبو با آنتی‌بیوتیک نیستاتین انجام شد. نتایج آزمایش‌های انتشار در دیسک و انتشار در چاهک نشان داد که اسانس بادرشبو تاثیر ضد‌قارچی قابل توجهی بر همه سویه‌های قارچی مورد مطالعه داشت. نتایج حداقل غلظت مهارکنندگی اسانس برای سویه‌های ساکارومایسس سرویزیه ،کاندیدا آلبیکانس، آسپرژیلوس نایجر، فوزاریوم سولانی و پنی‌سیلیوم اکسپنسوم به ترتیب 8، 16، 2، 8 و 4 میلی‌گرم بر میلی‌لیتر بود. حداقل غلظت کشندگی برای سویه‌های مذکور به ترتیب32، 64، 8، 16 و 32 میلی‌گرم بر میلی‌لیتر بود. همچنین نتایج حاصل از برهمکنش اسانس بادرشبو با آنتی‌بیوتیک ‌نیستاتین نیز حاکی از تاثیر هم‌افزایی اسانس مذکور با آنتی بیوتیک مورد آزمایش بود. با توجه به اثر قابل ملاحظه ضدقارچی مشاهده شده برای اسانس بادرشبو در پژوهش حاضر، می‌توان از آن در صنایع غذایی و دارویی استفاده کرد.
کلیدواژه‌ها
بادرشبو
نیستاتین
حداقل غلظت مهار کنندگی
حداقل غلظت کشندگی

موضوعات

عنوان مقاله English

Evaluation of the antifungal effect of Badrashboo (Dracocephalum moldavica) essential oil and its interaction with Nystatin on some fungal strains

نویسندگان English

Mitra Ghodsi Sheikhjan 1
ALI FAZLARA 2
Mohammad Hojjati 3
Behrooz Alizadeh behbahani 4
1 1- Ph.D. Student of Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 2- Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3 3- Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
4 4- Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

Although chemical antifungal preservatives are often used in various food products, the use of these substances has been limited due to their harmful effects on human health and the environment. Researchers have recently sought to replace these chemical compounds with natural and less dangerous substances. In this regard, using essential oils of medicinal plants can be considered a suitable alternative due to fewer side effects. Therefore, in the present research, after preparing the Badrashboo plant from the fields of Golmarz village located near Urmia city and drying it, extracting the essential oil from the Badrashboo was carried out using a Clevengerger, and the antifungal effect of Badrashboo essential oil on some important fungal strains with disc diffusion agar and well diffusion agar, minimum inhibitory concentration, minimum fungicidal concentration and the interaction of Badrashboo essential oil with Nystatin were performed. The results of disk diffusion agar and well diffusion agar tests showed that Badrashbo essential oil had a significant antifungal effect on all studied fungal strains. The results of the minimum inhibitory concentration of essential oil for strains of Saccharomyces cerevisiae, Candida albicans, Aspergillus niger, Fusarium solani, and Penicillium expansum were 8, 16, 2, 8, and 4 mg/ml, respectively. The minimum fungicidal concentration for the mentioned strains was 32, 64, 8, 16, and 32 mg/ml respectively. Also, the results of the interaction of Badrashboo essential oil with Nystatin indicated the synergistic effect of Badrashboo essential oil with Nystatin. Considering the significant antifungal effect observed for Badrashbo essential oil in the present study, it can be used in the food and pharmaceutical industries.

کلیدواژه‌ها English

Badrashboo
Minimum fungicidal concentration
Minimum Inhibitory Concentration
Nystatin
[1] Gomez sanchez, Aida., Enrique, Palou., & Pezmalo, Aureliolo.(2011). Antifungal activity evaluation of Mexican Oregano (Lippia berlandieri Schauer) essential oil on the growth of Aspergillus flavus by gaseous contact. Journal of Food Protection, 74(12), 2192–2198. doi:10.4315/0362-028X.JFP-11-308
[2] Alvesa M.J, Gonc.¸ Cruzb, MT., Cavaleiroa, C., Lopesb, MC., & Salgueiro, L. Chemical. (2010). antifungal and cytotoxic evaluation of the essential oil of Thymus zygis subsp. sylvestris. Journal of Industrial Crops and Products 32(2010) 70–75. doi:10.1016/j.indcrop.2010.03.005
[3] Silvaa, Filomena., Ferreiraa, Susana., Duartea, Anderia., Mendonc¸ DinaIa., & Domingues, Fernanda C. (2011). Antifungal activity of Coriandrum sativum essential oil, its mode of action against Candida species and potential synergism with amphotericin B. Journal of Phytomedicine 19 (2011) 42–47. doi:10.1016/j.phymed.2011.06.033
[4] Farahani, M., Shahidi, F., &Tabatabaei yazdi, F. (2019). Evaluation of antimicrobial activites of Satureja Hortensis L. essential Oil against some food born pathogenic and spoilage microorganism. Journal of Food Science Industry, 85 (15) 393-405. http://fsct.modares.ac.ir/article-7-16120-fa.html
[5] Khalil Karimzadeh-Asla, Khalil., Sefidkonb, Fatemeh., Ebrahimic, Mohsen., Farajpourcd, Mostafa & Marefatzadeh, Mani. (2015). Sequential Path Analysis of Effective Characters on the Essential Oil Yield of Dracocephalum moldavica L. Journal of Essential Oil Bearing Plants, 18(3), 702-712. http://dx.doi.org/10.1080/0972060X.2014.929046
[6] Holm, Yvonne., & Hiltunen., Raimo. (1988). Capillary Gas Chromatographic - Mass Spectrometric Determination of the Flavour Composition of Dragonhead (Dracocephalum moldavica L.). Journal of Flavour and fragrance. 3 (1988), 109–112.
[7] Teresa, Carolliny., Moreira Lustoza, Rodriguesa., Aline, Matilde Ferreira dos Santosb., Jéssica Paiva, de Moura., Jeremias Justo, Emídio., Paulo Sérgio, da Silva Pereira., Igor Mikael Alves, de Araujoa., Nikole Durand, Trigueiroa., Ana Karoline Silva, de Aquino Vitala., Marcus Tullius, Scottia., Luciana, Scottia., Alex France, & Messias, Monteiro. (2022). Physicochemical differences and antifungal activity of citral isomers: neral and geranial. Journal of M0l2net. 8, 2624-5078. https://mol2net-08.sciforum.net/
[8] Khadem Arabbaghi, E., Gurbuz, B., Afshar Pour Rezaeieh, K.,& Uyanik, M. (2014). GC/MS analysis of bioactive components of Dracocephalum moldavica L., treated by Boric Acid Doses. Journal of Tarım Bilimleri Araştırma Dergisi,7(1), 19-21. ISSN: 1308-3945, E-ISSN: 1308-027X, www.nobel.gen.tr.
[9] Dariusz Dziki, Dariusz., Cacak-Pietrzak, Grazyna., Gawlik-Dziki, Urszula., Sułek, Alicja., Kocira, Stawomir & Biernacka, Beata. (2019). Effect of Moldavian dragonhead (Dracocephalum moldavica L.) leaves on the baking properties of wheat flour and quality of bread. Journal of Food, 17 (1) 536-543. https://doi.org/10.1080/19476337.2019.1609587
[10] Keyvan Dastmalchi, H.J. Damien Dorman, Into Laakso, Raimo Hiltunen. (2007). Chemical composition and antioxidative activity of Moldavian balm (Dracocephalum moldavica L.) extracts. Journal of Food Science and Technology, 40 (2007). 1655–166. doi:10.1016/j.lwt.2006.11.013
[11] Maihemuti, Maimaitiming., Abulaiti, Remila., & Parha, Alimjan. (2019). Study on the protective effect of different extraction sites of Hyssopus officinalis and Dracocephalum moldavica combination on cerebral ischemia-reperfusion injury in rats. Journal of Traditional Medicine and Modern Medicine, 2 (4). 171–177. DOI: 10.1142/S2575900019500149
[12] Acimovic, M., Sovljanski, O., Seregelj, V., Pezo, L., Zheljazkov, VD., Ljujic, J., Tomic, A., Cetkovic, G., ´ Canadanovic-Brunet, J., Miljkovic, A., & Ljubodrag Vujisic. (2022) Chemical Composition, Antioxidant, and Antimicrobial Activity of Dracocephalum moldavica L. Essential Oil and Hydrolate. Journal of Plants,11(941), 1-16. Doi: 10.3390/plants11070941
[13] Farnoosh, Fakhari., Siavash Maktabi, Syavash., Fazlara3, Ali., Bavarsad, Neda., & Pormehdi Brojeni, Mahdi. (2022). Comparison of antimicrobial and antioxidant activities of essential oil and Nano-emulsion of Ferulago angulata (Chavir) collected from the western regions of Iran. Journal of Iran veterinary. 4(17), 71-147. DOI: 10.22055/IVJ.2020.234536.2271
[14] Rahmati Joneidabad,M., & Alizadeh Behbahani, B. (2021). Investigation of the antifungal effect of Myrtus communis essential oil on Penicillium digitatum and Penicillium italicum (the green and blue fungi of orange). Journal of Iranian Food Science industry. 109(17), 1-8. DOI 10.29252/fst.17.12,01
[15] Al-Ja’fari, Adel hadi., Vila, Roser., Freixa, Blanca., Tomi, Felix ., Casanova, Joseph., Costa, Joan., & Cañigueral a, Salvador. (2011). Composition and antifungal activity of the essential oil from the rhizome and roots of Ferula hermonis. Journal of Phytochemistry 72 (2011) 1406–1413. doi:10.1016/j.phytochem.2011.04.013
[16] Noshada, Mohammad., Hojjatia, Mohammad., & Alizadeh Behbahani, Behrooz. (2018). Black Zira essential oil: Chemical compositions and antimicrobial activity against the growth of some pathogenic strain causing infection. Journal of Microbial Pathogenesis. 116 (2018) 153–157. https://doi.org/10.1016/j.micpath.2018.01.026
[17] Alizadeh Behbahani, Behrooz ., Shahidi, Fakhri., Tabatabaei Yazdi, Farideh., & Mortazavi Mohebbat Mohebbi, Seyed Ali. (2016). Use of Plantago major seed mucilage as a novel edible coating incorporated with Anethum graveolens essential oil on shelf life extension of beef in refrigerated storage. Journal of Biological Macromolecules. 94 (2017),515-526. http://dx.doi.org/doi:10.1016/j.ijbiomac.2016.10.055
[18] Rahmati Joneidabad,M., & Alizadeh Behbahani, B. (2021). Identification of chemical compounds, antioxidant potential, and antifungal activity of Thymus daenensis essential oil against spoilage fungi causing apple rot. Journal of Iranian Food Science and Technology Research. 17(5), 691-700. DOI: https://doi.org/10.22067/ifstrj.v18i1.87595
[19] Jalil Sarghaleh, Sh., Alizadeh Behbahani, B., Hojjati, M., Vasiee, A., & Noshad, M. (2024). Antimicrobial effect of Prangos ferulacea aqueous extract on some pathogenic fungal species and its interaction with nystatin antibiotic. Journal of Food Science And Thecnology. 143(20), 140-149. DOI: 10.22034/FSCT.20.143.140
[20] Sonboli, A., Mojarrad, M., Gholipour, A., Nejad Ebrahimi, S., & Arman, M. (2008). Biological Activity and Composition of the Essential Oil of Dracocephalum moldavica L. Grown in Iran. Journal of Natural Product Communications, 3(9),1547-1550. Doi: 10.1177/1934578X0800300930
[21] Nazemisalman, B., Vahabi, S., Yazdinejad, A., Haghghi, F., Shabbuii Jam, M., & Heydari, F. (2017). Comparison of antimicrobial effect of Ziziphora tenuior, Dracocephalum moldavica, Ferula gummosa, and Prangos ferulacea essential oil with chlorhexidine on Enterococcus faecalis: An in vitro study. Journal of Dental Research, 15(2), 111-116. PMID: 29576774- PMCID: PMC5858069
[22] El-Baky, HHA., & El-Baroty, GS. (2007). Chemical and biological evaluation of the essential oil of Egyptian Moldavian balm. Journal of Advances in Food Sciences, 2(2), 74-80.
[23] Yu, H., Liu, M., Liu, Y., Qin, L., Jin, M., & Wang, Z. (2019) Antimicrobial Activity and Mechanism of Action of Dracocephalum moldavica L. Extracts Against Clinical Isolates of Staphylococcus aureus. Journal of Frontiers in Microbiology, 10(1249), 1-10. Doi: 10.3389/fmicb.2019.01249
[24] Haghighati F, Jafari S, Beyt EJ. (2003) Comparison of antimicrobial effects of ten herbal extracts with chlorhexidine on three different oral pathogens; an in vitro study. Hakim. 6(3): 71-6.
[25] Rahmati Joneidabad,M., Alizadeh Behbahani, B.,& Noshad, M. (2021). Antifungal effect of Satureja khuzestanica essential oil on Aspergillus niger, Botrytis cinerea, and Rhizopus stolonifer causing strawberry’s rot and mold. Journal of Iranian Food Science industry. 115(18), 171-180. DOI ‎ 10.52547/fsct.18.115.13
[26] Ghasemi, Mehdi., Radjabalizade, Keyvan., Gharari, Kia., Yaghoobi, Hashem., & Asgarpoor, Daryush (2016). Chemical Composition and in Vitro Antibacterial Activit y of Native Mentha longifolia Essential Oil from Ardabil, Iran on Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces viscosus. Journal of Infect Epidemiol Med. 2(2016), 11-14. DOI: 10.18869/modares.iem.2.1.11
[27] Jafarpour, Darnoosh., & Ataei, Parisa. (2022). Study the antifungal activities of Trachyspermum ammi, Satureja hortensis, and Mentha piperita essential oils against molds contaminating Iranian white cheese. Journal of Food Science and Technology. 124(19), 185-196. DOI : 10.52547/fsct.19.124.113
[28] Hoseyni, Sedigheh., Roodbar Mohammadi, Shahla., Joshaghani Hmid Reza. (2012). Evaluation of the antifungal activity of Carvacrol essential oil on standard strains of Candida albicans sensitive and resistant to Fuconazole.. Journal of Medical science. 5(2), 28-33.
[29] Barzegar, Hasan., Alizadeh Behbahani, Behrooz., & Noshad, Mohammad. (2021). Evaluation of total phenol and flavonoid contents, antioxidant and antimicrobial activity of Lawsonia inermis aqueous extract against some Gram - positive and Gram - negative bacteria. Journal of Food Science and Technology. 116(18), 327-335. DOI: 10.52547/fsct.18.116.327
[30] Noshad, Mohammad., & Alizadeh Behbahani, Behrooz. (2024). Evaluation of chemical properties and antimicrobial effect of Thymus trautvetteri essential oil on a number of bacteria causing infection and food poisoning: a laboratory study. Journal of Food Science and Technology. 145(20), 99-110. DOI: 10.22034/FSCT.20.145.99
[31] Noshad, Mohammad., & Alizadeh Behbahani, Behrooz. (2023). Antimicrobial activity of Datura stramonium ethanolic extract on some pathogenic bacteria causing infection and food poisoning in vitro. Journal of Food Science and Technology 142(20), 161-170.DOI: 10.22034/FSCT.20.142.161
[32] Vio-Michelis, Sofia., Apablaza-Hidalgo, Gaston., Gomaez, Miguel., Pena-Vera, Raul., & Montengro, Gloria. (2012). Antifungal activity of three Chilean plant extracts on Botrytis cinerea. Journal of Botanical Sciences. 90(2): 179-183.
[33] Alizadeh Behbahani, Behrooz., Tabatabaei Yazdi, Farideh.,. Shahidi, Fakhri.,& Mohebbi, Mohabbat. (2014). Antifungal Effect of Aqueous and Methanolic Avicennia Marina Leaves Extracts on Alternaria Alternata and Penicillium Citrinum. Journal of Rafsanjan medical science university. 12(12), 1015-1024. DOI: 20.1001.1.17353165.1392.12.12.5.1
[34] Ahmad Nejhad, Armin., Alizadeh Behbahani, Behrooz., Hojjati, Mohammad., Vasiee, Ali Reza., & Mehrnia, Mohammad Amin. (2024). Investigation of the inhibitory, fungicidal and interactive effects of the aqueous extract of Calotropis procera on Alternaria alternata, Alternaria solani, Saccharomyces cerevisiae, and Fusarium solani “in vitro ”. Journal of Food Science And Thecnology. 143(20), 204-214. DOI: 10.22034/FSCT.20.143.204
[35] Tanavar, Hadi., Barzegar, Hasan., Alizadeh Behbahani, Behrooz., & Mehrnia, Mohammad Amin. (2020). Evaluation of the antimicrobial activity of Menthapulegiumessential oil on some foodborne pathogens and its interaction with gentamicin and chloramphenicol in vitro. Journal of Food Science Industry, 97(16), 77-87. DOI: 10.29252/fsct.16.97.77
[36] Saffari Samani, Elnaz., Jooyandeh, Hossein., & Alizadeh Behbahani, Behrooz. (2020). Evaluation of reciprocal pharmaceutical effect and antimicrobial activity of Shirazi thyme essential oil against some Gram-positive and Gram-negative bacteria. Journal of Food Science Industry, 104(17), 1-11.DOI: 10.29252 /fsct. 17.07.01