قابلیت زنده‌مانی Lactiplantibacillus pentosus v390 در شرایط اسیدی و صفراوی، و ارزیابی فعالیت ضدمیکروبی و ایمنی آن

نویسندگان
بهروز علیزاده بهبهانی 1
حسین جوینده 2
پگاه نمازی 3
1 1. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
2 2. استاد، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
3 3. دانشجوی دکتری، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
10.22034/FSCT.21.153.192
چکیده
در این پژوهش، شناسایی مولکولی Lactiplantibacillus pentosus v390توسط تجزیه و تحلیل ژن rRNA S 16 به‌کمک پرایمرهای FYM27 و R1492 انجام شد. قابلیت زنده مانی سویه در شرایط اسیدی در pHهای 5/2، 5/3 و 5/4، و مقاومت به صفرا در غلظت های صفر، ۳/۰، ۵/۰ و ۷/۰ درصد بررسی شد. فعالیت آنتی‌اکسیدانی، جذب کلسترول، هیدروفوبیسیتی، پتانسیل تولید آنزیم DNase و آمین‌های بیوژنیک، فعالیت همولیتیک، و مقاومت به آنتی‌بیوتیک‌های رایج درمانی ارزیابی شد. اثر ضدمیکروبی سویه علیه باکتری‌های بیماری‌زا (Shigella dysenteriae، Salmonella enterica serovar Typhimurium، Escherichia coli، Staphylococcus aureus ، Listeria monocytogenes و Bacillus subtilis) به‌ روش انتشار در آگار به ‌کمک چاهک و دیسک بررسی گردید. نتایج نشان داد سویه L. pentosus v390 توانایی زنده‌مانی در pHهای مختلف را داشت اما با گذشت سه ساعت از نگهداری در pH 5/2 تعداد باکتری کاهش یافت. سویه مذکور قابلیت رشد در غلظت‌های مختلف نمک صفراوی را از خود نشان داد. L. pentosus v390 نسبت به آنتی‌بیوتیک‌ Nitrofurazone نیمه‌حساس، نسبت به آنتی‌بیوتیک‌های Nalidixic و Imipenem مقاوم و نسبت به آنتی‌بیوتیک‌های Vancocin، Gentamicin، Chloramphenical،Penicillin و Ciprofloxacin حساس بود. هیدروفوبیسیتی، فعالیت آنتی‌اکسیدانی (DPPH و ABTS)و میزان جذب کلسترول سویه به‌ترتیب 38/۰ ± ۵۰/46، 4۰/۰ ± 20/37، 45/۰ ± 9۰/39 و ۴7/۰ ± ۵0/36 درصد بود. تولید آنزیم DNase، آمین‌های بیوژنیک و فعالیت همولیتیک از سویه مشاهده نشد. L. pentosus v390 دارای اثر ضدمیکروبی بیشتری بر باکتری‌های گرم مثبت بود. نتایج نشان داد کهL. pentosus v390 دارای ویژگی‌های پروبیوتیکی مطلوبی است که پژوهش‌های بیشتر به‌ منظور تأیید قابلیت کاربرد آن در تولید محصولات غذایی ضروری است.
کلیدواژه‌ها
آمین‌های بیوژنیک
جذب کلسترول
فعالیت ضدباکتریایی
هیدروفوبیسیتی

موضوعات

عنوان مقاله English

The viability of Lactiplantibacillus pentosus v390 under acidic and bile conditions, and evaluation of its antimicrobial activity and safety

نویسندگان English

Behrooz Alizadeh behbahani 1
Hossein Jooyandeh 2
Pegah Namazi 3
1 1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 2Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
3 3PhD. student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

In this study, molecular identification of Lactiplantibacillus pentosus v390 was performed using 16S rRNA gene analysis with FYM27 and R1492 primers. The viability of the strain was assessed under acidic conditions at pH 5.2, 5.3, and 5.4, and resistance to bile at concentrations of 0%, 3.0%, 5.0%, and 7.0% was investigated. Antioxidant activity, cholesterol absorption, hydrophobicity, potential for DNase enzyme production, biogenic amines, hemolytic activity, and resistance to common therapeutic antibiotics were evaluated. The antimicrobial effect of the strain against pathogenic bacteria (Shigella dysenteriae, Salmonella enterica serovar Typhimurium, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Bacillus subtilis) was examined using the well and disk diffusion method. The results showed that the L. pentosus v390 strain had the ability to survive at different pH levels, but after three hours of storage at pH 5.2, the bacterial count decreased. The strain demonstrated growth capability at various bile salt concentrations. L. pentosus v390 was intermediate to the antibiotic Nitrofurazone, resistant to Nalidixic and Imipenem antibiotics, and sensitive to Vancocin, Gentamicin, Chloramphenicol, Penicillin, and Ciprofloxacin antibiotics. The hydrophobicity, antioxidant activity (DPPH and ABTS), and cholesterol absorption of the strain were 38.0 ± 50.46%, 40.0 ± 20.37%, 45.0 ± 9.39%, and 47.0 ± 50.36%, respectively. No DNase enzyme production, biogenic amines, or hemolytic activity were observed from the strain. L. pentosus v390 exhibited stronger antimicrobial effects against Gram-positive bacteria. The results indicated that L. pentosus v390 has desirable probiotic properties that require further research to confirm its application potential in food product development.

کلیدواژه‌ها English

Biogenic amines
Cholesterol adsorption
Antibacterial activity
Hydrophobicity
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