بهینه سازی تولید زیست توده توسط‌ Monascus purpureus در محیط‌ کشت‌ حاوی لجن لبنی

نویسندگان
سمیرا مرادی
سیدعلی مرتضوی
دانشگاه فردوسی مشهد
10.22034/FSCT.21.150.182
چکیده
ترکیبات ارزان قیمت و ضایعات صنایع مختلف دارای مجموعه‌ای از ترکیبات مغذی هستند که می‌توان از آن‌ها به عنوان بستر کشت سویه‌های مختلف جهت تولید متابولیت‌هایی با ارزش غذایی بالا و گران قیمت‌ تولید کرد. تولید رنگدانه از منبع میکروبی به دلیل ایمن بودن برای سلامتی انسان مورد توجه است. در این مطالعه با هدف تولید رنگدانه قرمز ازکپک Monascus purpureus ابتدا تاثیر فاکتورهای محیط‌ کشت‌ پوتیتو دکستروز براث، عصاره مخمر- ساکارز، لجن‌ لبنی‌، ‌ کنجاله‌ سویا، ملاس نیشکر، آب پنیر، دما، گلوکز، مونو سدیم‌ گلوتامات و سولفات آمونیوم بر تولید زیست توده (معیاری از تولید رنگدانه)‌ از طریق‌ طرح پلاکت‌-برمن‌ مورد بررسی‌ قرارگرفت‌. سپس بهینه سازی فاکتورها براساس طرح مربع مرکزی انجام شد. براساس نتایج‌ بدست‌‌آمده محیط کشت‌ پایه‌ لجن‌ لبنی، گلوکز، مونوسدیم‌گلوتامات و دما تاثیر مثبتی‌ بر رشــد و تولید بیومس‌ (تولید رنگدانه‌) داشــتند. با بهینه‌سازی، تولید زیست توده‌ در لجن‌ لبنی‌ ١٠ در صد، مونوسدیم‌ گلوتامات ٩٩٩/٠ درصد، دمای ٢٧ درجه‌ سانتی‌گراد و ٨٣/٩ درصد گلوکز با تولید مقدار ١٥/٢٦ گرم بر لیتر زیســت‌ توده به‌ عنوان حالت‌ بهینه‌ بدســت‌ آمد. نتایج نشاندهنده قابلیت ضایعات لجن لبنی در رشد سویه میکروبی و تولید محصول قابل توجه می‌باشد.
کلیدواژه‌ها
زیست توده
Monascus purpureus
بهینه‌سازی
لجن لبنی

موضوعات

عنوان مقاله English

Optimization of biomass production by Monascus purpureus in culture medium containing dairy sludge

نویسندگان English

Samira Moradi
Seyed Ali Mortazavi
Ferdowsi University of Mashhad
چکیده English

Cheap compounds and wastes of various industries have a set of nutritious compounds that can be used as a culture medium for different strains to produce metabolites with high nutritional value and expensive. Pigment production from microbial source is of interest because it is safe for human health. In this study, with the aim of producing red pigment from the mold Monascus purpureus, firstly, the effect of culture medium factors, potato dextrose broth, yeast extract-sucrose, dairy sludge, soybean meal, sugar cane molasses, whey, temperature, glucose, monosodium glutamate and ammonium sulfate on production biomass (a measure of pigment production) was evaluated through Plackett-Burman design. Then the factors were optimized based on the central square design. Based on the obtained results, dairy sludge base culture medium, glucose, monosodium glutamate and temperature had a positive effect on growth and biomass production (pigment production). With optimization, biomass production in 10% dairy sludge, 0.999% monosodium glutamate, 27°C temperature and 9.83% glucose were obtained with the production of 26.15 g/l of biomass as the optimal state. The results show the capability of dairy sludge waste in the growth of microbial strains and production of significant products.

کلیدواژه‌ها English

Biomass
Monascus purpureus
Optimization
Dairy sludge
[1] N. Kumara, A. Lim, C.M. Lim, M.I. Petra, P. Ekanayake, Recent progress and utilization of natural pigments in dye sensitized solar cells: A review, Renewable and Sustainable Energy Reviews 78 (2017) 301-317.
[2] F. Vendruscolo, R.M.M. Bühler, J.C. de Carvalho, D. de Oliveira, D.E. Moritz, W. Schmidell, J.L. Ninow, Monascus: a reality on the production and application of microbial pigments, Applied Biochemistry and Biotechnology 178 (2016) 211-223.
[3] C. Agboyibor, W.-B. Kong, D. Chen, A.-M. Zhang, S.-Q. Niu, Monascus pigments production, composition, bioactivity and its application: A review, Biocatalysis and Agricultural Biotechnology 16 (2018) 433-447.
[4] G. Mukherjee, S.K. Singh, Purification and characterization of a new red pigment from Monascus purpureus in submerged fermentation, Process Biochemistry 46(1) (2011) 188-192.
[5] J. Liu, Y. Luo, T. Guo, C. Tang, X. Chai, W. Zhao, J. Bai, Q. Lin, Cost-effective pigment production by Monascus purpureus using rice straw hydrolysate as substrate in submerged fermentation, Journal of bioscience and bioengineering 129(2) (2020) 229-236.
[6] R. Panesar, S. Kaur, P.S. Panesar, Production of microbial pigments utilizing agro-industrial waste: a review, Current Opinion in Food Science 1 (2015) 70-76.
[7] S. Moradi, F. Zeraatpisheh, F. Tabatabaee-Yazdi, Investigation of lactic acid production in optimized dairy wastewater culture medium, Biomass Conversion and Biorefinery (2022) 1-12.
[8] F. Falah, A. Vasiee, F. Tabatabaei-Yazdi, S. Moradi, S. Sabahi, Optimization of γ-aminobutyric acid (GABA) production by Lactobacillus spp. from agro-food waste, Biomass Conversion and Biorefinery (2022) 1-13.
[9] H. Keivani, M. Jahadi, N. Ghasemisepero, Optimizing submerged cultivation for the production of red pigments by Monascus purpureus on soybean meals using response surface methodology, Applied Food Biotechnology 7(3) (2020) 143-152.
[10] S. Silbir, Y. Goksungur, Natural red pigment production by Monascus purpureus in submerged fermentation systems using a food industry waste: Brewer’s spent grain, Foods 8(5) (2019) 161.
[11] P. Gong, R. Shi, Y. Liu, Q. Luo, C. Wang, W. Chen, Recent advances in Monascus pigments produced by Monascus purpureus: Biosynthesis, fermentation, function, and application, LWT (2023) 115162.
[12] I. Srianta, E. Zubaidah, T. Estiasih, M. Yamada, Comparison of Monascus purpureus growth, pigment production and composition on different cereal substrates with solid state fermentation, Biocatalysis and Agricultural Biotechnology 7 (2016) 181-186.
[13] X. Xiong, Z. Zhen, Y. Liu, M. Gao, S. Wang, L. Li, J. Zhang, Low‐frequency magnetic field of appropriate strengths changed secondary metabolite production and Na+ concentration of intracellular and extracellular Monascus purpureus, Bioelectromagnetics 41(4) (2020) 289-297.
[14] X. Chen, J. Yan, J. Chen, R. Gui, Y. Wu, N. Li, Potato pomace: An efficient resource for Monascus pigments production through solid-state fermentation, Journal of bioscience and bioengineering 132(2) (2021) 167-173.
[15] G.A.-E. Mahmoud, H.A. Soltan, W.M. Abdel-Aleem, S.A. Osman, Safe natural bio-pigment production by Monascus purpureus using mixed carbon sources with cytotoxicity evaluation on root tips of Allium cepa L, Journal of Food Science and Technology 58 (2021) 2516-2527.
[16] D. Mehri, N.A. Perendeci, Y. Goksungur, Utilization of whey for red pigment production by Monascus purpureus in submerged fermentation, Fermentation 7(2) (2021) 75.
[17] T. Orak, O. Caglar, S. Ortucu, H. Ozkan, M. Taskin, Chicken feather peptone: A new alternative nitrogen source for pigment production by Monascus purpureus, Journal of biotechnology 271 (2018) 56-62.
[18] Y. Hamdiyati, K. Kusnadi, L.A. Yuliani, Effect of Monascus purpureus inoculum concentration on pigment production in jackfruit seed flour substrate, AIP Conference Proceedings, AIP Publishing, 2016.
[19] S. Erkaya, N.P. Arslan, T. Orak, N. Esim, M. Taskin, Evaluation of tyrosol and farnesol as inducer in pigment production by Monascus purpureus ATCC16365, Journal of basic microbiology 60(8) (2020) 669-678.