کاربرد میکرولیپوزوم حاوی عصاره برگ فرولا بر ماندگاری همبرگر طی دوره نگهداری در یخچال

نویسندگان
محمد سلگی 1
مریم اثنی عشری 2
رضا فرهمندفر 3
1 دانشجوی کارشناسی ارشد علوم و صنایع غذایی، موسسه آموزش عالی خزر محمودآباد، ایران
2 استادیار بخش تحقیقات فراوری تولیدات دامی، موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویجکشاورزی، کرج، ایران
3 2دانشیار گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.
10.22034/FSCT.21.146.195
چکیده
با وجود توسعه روش­های افزایش ماندگاری و ایمنی فرآورده­های غذایی، همچنان زیان اقتصادی ناشی از فساد مواد غذایی از چالش­های اصلی محسوب می­شود. با توجه به فرهنگ سازی استفاده از مواد طبیعی و غذاهای فراسودمند، تمایل مصرف­کننده به مواد غذایی طبیعی با ماندگاری بالا افزایش یافته است. ترکیبات فنلی همچون بسیاری از ترکیبات زیست فعال به تدریج غیر فعال می­گردد و معمولا پس طعم تلخی را در مواد غذایی ایجاد می­کنند. میکرولیپوزوم یکی از راهکارهای موثر برای افزایش پایداری و کاهش طعم نامطلوب ترکیبات بیواکتیو محسوب می­شود. در این پژوهش، عصاره برگ فرولا با اتانول استخراج شد و خصوصیات فنلی و فلاونوئیدی آن تعیین شد. سپس خصوصیات آنتی اکسیدانی عصاره به روش DPPH و بتا کاروتن-اسید لینولئیک در سطوح غلظتی مختلف (400، 800، 1600 و 3200 PPM) تعیین شد. سپس، به صورت لیپوزوم به همبرگر اضافه شد و خصوصیات اکسایشی، میکروبی و حسی و میزان رهایش ترکیبات فنلی در همبرگر طی دوره 15 روز مورد بررسی قرار گرفت. نتایج حاصل نشان داد که محتوی فنل و فلاونوئید کل عصاره برگ فرولا به ترتیب 8/5 ± 67/270 میلی­گرم اسید گالیک بر گرم عصاره و 65/5 ±81/160 میلی­گرم کوئرستین در هر گرم از عصاره بود. با افزایش غلظت عصاره فرولا، میزان مهار رادیکال آزاد DPPH از 73/33 به 42/84 درصد و بتا کاروتن- اسید لینولئیک از 56/32 به 90/74 درصد از غلظت 400 تا 3200 PPM افزایش می­یابد. نتایج بدست آمده بر روی ماندگاری همبرگر نشان داد بیشترین رشد میکروبی و اکسایش لیپیدی در نمونه شاهد مشاهده شد و کمترین مقدار رشد در تیمار حاوی عصاره 3200 PPM عصاره فرولا دیده شد. براساس آزمون اکسایشی و حسی، در صورت افزودن میکرولیپوزوم عصاره برگ فرولا در غلظت 1600 PPM می­توان زمان ماندگاری همبرگر را به طور قابل توجهی در یخچال افزایش داد.
کلیدواژه‌ها
فرولا
آنتی اکسیدانی
ترکیبات فنلی
ماندگاری
همبرگر

موضوعات

عنوان مقاله English

Application of microliposome of ferula leaves extract on shelf life of beef burger during refrigerated storage

نویسندگان English

Mohammad Solgi 1
Maryam Asnaashari 2
Reza Farahmandfara 3
1 Master student, Department of Food Science and Technology, Khazar Institute of Higher Education, Mahmoudabad, Iran
2 Assistant Professor, Department of Animal Processing, Animal Science Research Institute of Iran (ASRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3 Associate Professor, Department of Food Science and Technology, Sari Agricultural Sciences & Natural Resources University (SANRU), Sari, Iran
چکیده English

Despite the development of methods to increase the shelf life and safety of food products, the economic loss caused by food spoilage is still considered one of the main challenges of food industry. Due to the culture of the use of natural products and functional foods, the consumer's desire for natural products with extended shelf life has increased. Phenolic compounds, like many bioactive compounds, gradually become inactive and usually cause a bitter taste in food. Microliposome is one of the effective solutions to increase the stability and reduce the unpleasant taste of bioactive compounds. In this research, the Ferula leaves was extracted with ethanol and its phenolic and flavonoid properties were determined. Then, the antioxidant properties of the extract were determined by DPPH and β-carotene/linoleic acid assay at different concentration (400, 800, 1600 and 3200 PPM). Then it was added to beef burger in the form of liposomes and the oxidative, microbial and sensory characteristics and the release rate of phenolic compounds in beef burger were investigated over two weeks’ storage. The results showed that the total phenolic and flavonoid content of the Ferula leaves extract was 270.67 ± 5.8 mgGA/ g extract and 160.81±5.65 mg Quercetin/g extract, respectively. With the increase in the concentration of Ferula extract, the DPPH radical inhibition increases from 33.73 to 84.42% and β-carotene-linoleic acid from 32.56 to 74.90% at 400 to 3200 PPM. The results obtained on the shelf life of beef burger showed that the highest microbial growth and lipid oxidation were observed in the control, and the lowest one was observed in the sample containing 3200 PPM Ferula extract. Based on the oxidation test and sensory evaluation, adding microliposome of Ferula leaves extract at 1600 PPM can significantly increase the shelf life of beef burger in the refrigerator.

کلیدواژه‌ها English

Ferula
Antioxidant
phenolic compounds
shelf life
beef burger
1. Solouki, M., Mehdikhani, H., Zeinali, H., & Emamjomeh, A. (2008). Study of genetic diversity in Chamomile (Matricaria chamomilla) based on morphological traits and molecular markers. Scientia Horticulturae, 117(3), 281-287.
2. Javidnia, K., Miri, R., Kamalinejad, M., & Edraki, N. (2005). Chemical composition of Ferula persica Wild. essential oil from Iran. Flavour and Fragrance Journal, 20(6), 605-606.
3. Salehi, M., Naghavi, M. R., & Bahmankar, M. (2019). A review of Ferula species: Biochemical characteristics, pharmaceutical and industrial applications, and suggestions for biotechnologists. Industrial Crops and Products, 139, 111511.
4. Nazari, Z. E., & Iranshahi, M. (2011). Biologically active sesquiterpene coumarins from Ferula species. Phytotherapy Research, 25(3), 315-323.
5. Dastan, D., Salehi, P., Aliahmadi, A., Gohari, A. R., Maroofi, H., & Ardalan, A. (2016). New coumarin derivatives from Ferula pseudalliacea with antibacterial activity. Natural Product Research, 30(24), 2747-2753.
6. Bagheri, S. M., Hedesh, S. T., Mirjalili, A., & Dashti-R, M. H. (2016). Evaluation of anti-inflammatory and some possible mechanisms of antinociceptive effect of Ferula assa foetida oleo gum resin. Journal of Evidence-based Complementary & Alternative Medicine, 21(4), 271-276.
7. Chandran, S., Sakthivel, M., Thirumavalavan, M., Thota, J. R., Mariappanadar, V., & Raman, P. (2017). A facile approach to the isolation of proteins in Ferula asafoetida and their enzyme stabilizing, anti-microbial and anti-oxidant activity. International Journal of Biological Macromolecules, 102, 1211-1219.
8. Perveen, I., Raza, M. A., Iqbal, T., Naz, I., Sehar, S., & Ahmed, S. (2017). Isolation of anticancer and antimicrobial metabolites from Epicoccum nigrum; endophyte of Ferula sumbul. Microbial Pathogenesis, 110, 214-224.
9. Lopez-Polo, J., Monasterio, A., Cantero-López, P., & Osorio, F. A. (2021). Combining edible coatings technology and nanoencapsulation for food application: A brief review with an emphasis on nanoliposomes. Food Research International, 145, 110402.
10. Nahr, F. K., Ghanbarzadeh, B., Hamishehkar, H., Kafil, H. S., Hoseini, M., & Moghadam, B. E. (2019). Investigation of physicochemical properties of essential oil loaded nanoliposome for enrichment purposes. LWT, 105, 282-289.
11. McAfee, A. J., McSorley, E. M., Cuskelly, G. J., Moss, B. W., Wallace, J. M., Bonham, M. P., & Fearon, A. M. (2010). Red meat consumption: An overview of the risks and benefits. Meat Science, 84(1), 1-13.
12. Godfray, H. C. J., Aveyard, P., Garnett, T., Hall, J. W., Key, T. J., Lorimer, J., . . . Jebb, S. A. (2018). Meat Consumption, Health, and the Environment. Science, 361(6399), eaam5324.
13. Asnaashari, M., Emami, S. A., & Tayarani-Najaran, Z. (2023). The effect of Hashemi brown and white rice extracts and γ-oryzanol on proliferation and estrogenic activity induced by zearalenone in MCF-7 cells. Journal of Food Measurement and Characterization, 1-14.
14. Tometri, S. S., Ahmady, M., Ariaii, P., & Soltani, M. S. (2020). Extraction and encapsulation of Laurus nobilis leaf extract with nano-liposome and its effect on oxidative, microbial, bacterial and sensory properties of minced beef. Journal of Food Measurement and Characterization, 14, 3333-3344.
15. Farahmandfar, R., Asnaashari, M., Asadi, Y., & Beyranvand, B. (2019). Comparison of bioactive compounds of matricaria recutita extracted by ultrasound and maceration and their effects on preventing sunflower oil during frying. Current Nutrition & Food Science, 15(2), 156-164.
16. Asnaashari, M., Emami, S. A., & Tayarani-Najaran, Z. (2023). The effect of Hashemi brown and white rice extracts and γ-oryzanol on proliferation and estrogenic activity induced by zearalenone in MCF-7 cells. Journal of Food Measurement and Characterization, 1-14.
17. Nasri, R., Younes, I., Jridi, M., Trigui, M., Bougatef, A., Nedjar-Arroume, N., . . . Karra-Châabouni, M. (2013). ACE inhibitory and antioxidative activities of Goby (Zosterissessor ophiocephalus) fish protein hydrolysates: Effect on meat lipid oxidation. Food Research International, 54(1), 552-561.
18. Jiménez, A., Sánchez-González, L., Desobry, S., Chiralt, A., & Tehrany, E. A. (2014). Influence of nanoliposomes incorporation on properties of film forming dispersions and films based on corn starch and sodium caseinate. Food Hydrocolloids, 35, 159-169.
19. Farahmandfar, R., Amini, A., Faghih Nasiri, S., & Asnaashari, M. (2018). Influence of Mentha piperita L. extract in the quality of soybean oil during microwave heating. Iranian Journal of food science and technology, 15(75), 201-216.
20. Pabast, M., Shariatifar, N., Beikzadeh, S., & Jahed, G. (2018). Effects of chitosan coatings incorporating with free or nano-encapsulated Satureja plant essential oil on quality characteristics of lamb meat. Food Control, 91, 185-192.
21. Homayounpour, P., Alizadeh Sani, M., & Shariatifar, N. (2021). Application of nano‐encapsulated Allium sativum L. essential oil to increase the shelf life of hamburger at refrigerated temperature with analysis of microbial and physical properties. Journal of Food Processing and Preservation, 45(11), e15907.
22. Cosme, P., Rodríguez, A. B., Espino, J., & Garrido, M. (2020). Plant phenolics: Bioavailability as a key determinant of their potential health-promoting applications. Antioxidants, 9(12), 1263.
23. Zhang, Y., Cai, P., Cheng, G., & Zhang, Y. (2022). A brief review of phenolic compounds identified from plants: Their extraction, analysis, and biological activity. Natural Product Communications, 17(1), 1934578X211069721.
24. Asnaashari, M., Farahmandfara, R., & Karamali, F. (2023). Biodegradable packaging based on chitosan-potato starch biopolymer containing Apium graveolens seed extract for chicken fillets. Journal of Food Science and Technology (Iran), 20(137), 111-128.
25. Farahmandfar, R., Naeli, M. H., Naderi, M., & Asnaashari, M. (2019). Stabilizing corn oil using the lemon balm (Melissa officinalis) antioxidants extracted by subcritical water. Journal of Food Science and Technology, 56, 695-704.
26. Yekta, M. M., Rezaei, M., Nouri, L., Azizi, M. H., Jabbari, M., Eş, I., & Khaneghah, A. M. (2020). Antimicrobial and antioxidant properties of burgers with quinoa peptide‐loaded nanoliposomes. Journal of Food Safety, 40(2), e12753.
27. Tekce, E., Çınar, K., Bayraktar, B., Takma, Ç., & Gül, M. (2020). Effects of an essential oil mixture added to drinking water for temperature-stressed broilers: performance, meat quality, and thiobarbituric acid-reactive substances. Journal of Applied Poultry Research, 29(1), 77-84
28. Ghoturi, S., Ojagh, S. M., Hasani, M., Alishahi, A., & Hasani, S. (2023). Effect of addition of fish oil nanoliposomes on the technological and nutrition quality of beef burgers over storage at 4 C. Utilization and Cultivation of Aquatics, 12(1), 143-162.
29. Eslamian Amiri, M., Ahmady, M., Ariaii, P., Golestan, L., & Ghorbani‐HasanSaraei, A. (2021). Use composite coating of chitosan‐chia seed gum enriched with microliposomes of Bay laurel essential oil to increase the shelf life of quail fillets. Food Science & Nutrition, 9(12), 6524-6537.
30. Machado, A. R., Pinheiro, A. C., Vicente, A. A., Souza-Soares, L. A., & Cerqueira, M. A. (2019). Liposomes loaded with phenolic extracts of Spirulina LEB-18: Physicochemical characterization and behavior under simulated gastrointestinal conditions. Food Research International, 120, 656-667.
31. Rashidinejad, A., Birch, E. J., Sun-Waterhouse, D., & Everett, D. W. (2014). Delivery of green tea catechin and epigallocatechin gallate in liposomes incorporated into low-fat hard cheese. Food Chemistry, 156, 176-183.
32. Friedman, M., Levin, C., Lee, S. U., & Kozukue, N. (2009). Stability of green tea catechins in commercial tea leaves during storage for 6 months. Journal of Food Science, 74(2), H47-H51.
33. Manafi, D. M., Hadad, K. M., Azadmard, D. S., Valizadeh, H., & Tabatabaei, Y. F. (2018). Preparation and some characteristics of nano liposomes containing olive leaf extract.
34. Salamatian, M., Zahedi, Y., & Saddel, R. (2023). Production and Evaluation of Nanoliposomes Loaded with Capparis spinosa Extract. Iranian Food Science and Technology Research Journal, 19(1), 169-180.
35. Aala, J., Ahmadi, M., Golestan, L., & Shahidi, S. A. (2022). The effect of free form and microcoating with nanoliposomes of Laurus nobilis and Rosemary leaves extracts on the behavior of some chemical indicators of spoilage in Silver carp fish kept at refrigerator temperature. Journal of Food Science and Technology (Iran), 19(131), 275-289.
36. Emami, S., Ahmadi, M., Roozbeh Nasiraie, L., Shahidi, S.-A., & Jafarizadeh‑Malmiri, H. (2023). The effect of free and encapsulated essential oil and extract of cinnamon with nanoliposome on Listeria monocytogenes and Escherichia coli inoculated into ground beef. Journal of Food Science and Technology (Iran), 19(133), 1-16.
37. Lima, M. d. C., de Sousa, C. P., Fernandez-Prada, C., Harel, J., Dubreuil, J., & De Souza, E. (2019). A review of the current evidence of fruit phenolic compounds as potential antimicrobials against pathogenic bacteria. Microbial Pathogenesis, 130, 259-270.
38. Kalogianni, A. I., Lazou, T., Bossis, I., & Gelasakis, A. I. (2020). Natural phenolic compounds for the control of oxidation, bacterial spoilage, and foodborne pathogens in meat. Foods, 9(6), 794.
39. Babuskin, S., Babu, P. A. S., Sasikala, M., Sabina, K., Archana, G., Sivarajan, M., & Sukumar, M. (2014). Antimicrobial and antioxidant effects of spice extracts on the shelf life extension of raw chicken meat. International Journal of Food Microbiology, 171, 32-40.
40. Shahbazi, Y., Shavisi, N., & Mohebi, E. (2016). Effects of Z iziphora clinopodioides Essential Oil and Nisin, Both Separately and in Combination, to Extend Shelf Life and Control E scherichia coli O 157: H 7 and S taphylococcus aureus in Raw Beef Patty during Refrigerated Storage. Journal of Food Safety, 36(2), 227-236.
41. Safari, R., Raftani Amiri, Z., & Esmaeilzadeh Kenari, R. (2018). Optimizing the extraction of phycocyanin pigment from Spirulina platensis algae and investigating the qualitative properties of the encapsulated pigment. PhD thesis, Sari Agricultural Sciences and Natural Resources University.
42. Rather, S. A., Masoodi, F., Akhter, R., Gani, A., Wani, S., & Malik, A. (2016). Effects of guar gum as fat replacer on some quality parameters of mutton goshtaba, a traditional Indian meat product. Small Ruminant Research, 137, 169-176.