شناسایی ترکیبات شیمیایی اسانس رازیانه، قدرت آنتی اکسیدانی و اثر ضدقارچی آن بر کپک‌های عامل فساد پس از برداشت میوه انگور

نویسندگان
1 استادیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 دانشیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
چکیده
انگور میوه­ای بسیار فسادپذیر است که دارای عمر نگهداری کوتاهی می­باشد. به دلیل افزایش سطح آگاهی مصرف­کنندگان تمایل به استفاده از ترکیبات نگهدارنده طبیعی به جای آفت‌کش‌ها و نگهدارنده­های شیمیایی افزایش یافته است. اسانس­های گیاهی به دلیل ترکیبات مؤثره بالقوه از مناسب­ترین ترکیبات طبیعی مورد استفاده به شمار می­آیند. از این رو، هدف از این پژوهش، شناسایی ترکیبات شیمایی اسانس رازیانه، تعیین میزان فنول و فلاونوئید آن، بررسی فعالیت آنتی‌اکسیدانی و فعالیت ضد قارچی این اسانس بر آن بر تعدادی از کپک‌های عامل فساد پس از برداشت میوه انگور بود. ترکیبات شناسایی شده در اسانس رازیانه شامل limonene، fenchone، p-allylanisole و anethole بود که در مجموع 60/97 درصد کل اسانس را تشکیل دادند. میزان فنول کل اسانس رازیانه برابر با 29/26 میلی­گرم گالیک­ اسید در گرم اسانس و میزان فلاونوئید کل آن برابر 23/19 میلی­گرم کوئرستین در گرم اسانس به دست آمد. خاصیت آنتــی اکــسیدانی اسانس این گیاه در روش مهــار رادیکال­هـای آزاد DPPH، ABTS و رنگ­بری بتاکاروتن- لینولئیک اسید بـه ترتیـب 56/38، 12/42 و 30/30 درصد به دست آمد. همچنین در بررسی اثر ضد قارچی بر کپک­های عامل فساد مشخص گردید که اسانس رازیانه قادر به مهار و کنترل کپک­های ریزوپوس استولونیفر، آسپرژیلوس نایجر و بوتریتیس سینه­را می­باشد. با توجه به ترکیبات ضد میکروبی و آنتی اکسیدانی موجود در رازیانه، اسانس این گیاه می­تواند به عنوان یک ترکیب با خاصیت عملگرایی در افزایش عمر نگهداری محصولات کشاورزی از جمله انگور مورد استفاده قرار گیرد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Identification of chemical compounds of Foeniculum vulgare essential oil, antioxidant power, and its antifungal effect on postharvest grape spoilage molds

نویسندگان English

Mostafa Rahmati 1
Fatemeh Borna 1
Mokhtar Heidari 2
1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

Grape is a very perishable fruit that has a short shelf life. Due to the increase in consumer awareness, the tendency to use natural preservative compounds instead of pesticides and chemical preservatives has increased. Plant essential oils are considered to be the most appropriate natural compounds due to their potentially effective compounds. Therefore, the aim of this research was to identify the chemical compounds of Foeniculum vulgare essential oil, determine its phenolic and flavonoid content, and investigate the antioxidant and antifungal activity of this essential oil against a number of molds that cause spoilage after grape fruit harvesting. The compounds identified in F. vulgare essential oil included limonene, fenchone, p-allylanisole and anethole, which made up 97.60% of the total essential oil. The total phenol content of F. vulgare essential oil was equal to 26.29 mg GAE/g and the total flavonoid content was equal to 19.23 mg QE/g. The antioxidant properties of the essential oil of this plant were obtained by DPPH, ABTS and beta-carotene-linoleic acid decolorization methods, respectively, 38.56, 42.12, and 30.30%. Also, , it was found that Foeniculum vulgare essential oil is capable of inhibiting and controlling Rhizopus stolonifera, Aspergillus niger, and Botrytis cinerea. Due to the antimicrobial and antioxidant compounds present in F. vulgare, the essential oil of this plant can be used as a compound with practical properties to increase the shelf life of agricultural products, including grapes.

کلیدواژه‌ها English

Foeniculum vulgare essential oil
total phenol and flavonoid
anti-fungal and antioxidant effect
Grape
[1] Mohammadi, S., Aroiee, H., Tehranifar, A., & Jahanbakhsh, v. (2012). Application of essential oils in control postharvest decay of strawberry fruit caused by Bottrytis cinerea fungus. Postharvest Physiology and Technology of Horticultural Crops, 9(2), 55-73.
[2] Dehestani-Ardakani, M., & Mostofi, Y. (2019). Maintaining Quality Properties of Grape CV.‘Bidaneh Ghermez’by Chitosan Edible Coating, Thymus Essential Oil and their Concomitant Application. Isfahan University of Technology-Journal of Crop Production and Processing, 9(3), 165-176.
[3] Welke, J. E. (2019). Fungal and mycotoxin problems in grape juice and wine industries. Current Opinion in Food Science, 29, 7-13. doi:https://doi.org/10.1016/j.cofs.2019.06.009.
[4] Naeimi, S., & Zare, R. (2013). Evaluation of indigenous Trichoderma spp. isolates in biological control of Botrytis cinerea, the causal agent of strawberry gray mold disease. BioControl in Plant Protection, 1(2), 55-74. doi:10.22092/bcpp.2013.100609.
[5] Karami, M. J. (2011). Effect of Grape Guard Pads on Extended Storage Life of Fruits of Rotabi and Siah-e-Samarghandi Grape Cultivars. Seed and Plant Production Journal, 27(3), 335-353. doi:10.22092/sppj.2017.110441
[6] Rahmati-Joneidabad, M., Alizadeh Behbahani, B., & Noshad, M. (2023). Determination of antioxidant activity, and antifungal effect of Ferula persica L hydroalcoholic extract on some fungal strains causing strawberry and grape fruits rot “in vitro”. Research in Plant Metabolites, 1(2), 5-15.
[7] Saeedi, M., Ebrahimzadeh, M. A., Morteza-Semnani, K., Âkha, O., & Rabiei, K. H. (2010). Evaluation of antibacterial effect of ethanolic extract of Foeniculum vulgare Mill. Journal of Mazandaran University of Medical Sciences, 20(77), 88-91.
[8] Moslemi, L., Bakhredi, R., Hassani, S., & Khaleghi Nezhad, K. (2013). The Effect of Fennel Juice on Primary Dysmenorrhea. Quarterly Journal of Health Breeze, 1(4), 15-20
[9] Noshad, M., & Falah, F. (2020). Study the chemical composition of essential oil of Foeniculum vulgare and antioxidant activity and its cell toxicity. Journal of food science and technology (Iran), 17(104), 124-133.
[10] Bahrami, S., Alizadeh Doughikollaee, E., & Shahriari moghadam, M. (2018). Effect of Foeniculum vulgare seed essential oil on the Staphylococcus aureus in minced Cyprinus carpio. Food Hygiene, 8(4 (32)), 1-13.
[11] Anwar, F., Ali, M., Hussain, A. I., & Shahid, M. (2009). Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare Mill.) seeds from Pakistan. Flavour and Fragrance Journal, 24(4), 170-176. doi:https://doi.org/10.1002/ffj.1929
[12] Roby, M. H. H., Sarhan, M. A., Selim, K. A.-H., & Khalel, K. I. (2013). Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare L.) and chamomile (Matricaria chamomilla L.). Industrial Crops and Products, 44, 437-445.
[13] Heydari, S., Jooyandeh, H., Alizadeh Behbahani, B., & Noshad, M. (2020). The impact of Qodume Shirazi seed mucilage‐based edible coating containing lavender essential oil on the quality enhancement and shelf life improvement of fresh ostrich meat: An experimental and modeling study. Food Science & Nutrition, 8(12), 6497-6512.
[14] Falah, F., Shirani, K., Vasiee, A., Tabatabaee Yazdi, F., & Alizadeh Behbahani, B. (2021). In vitro screening of phytochemicals, antioxidant, antimicrobial, and cytotoxic activity of Echinops setifer extract. Biocatalysis and Agricultural Biotechnology, 35, 102102.
[15] Zanganeh, H., Mortazavi, S. A., Shahidi, F., & Alizadeh Behbahani, B. (2021). Evaluation of the chemical and antibacterial properties of Citrus paradise essential oil and its application in Lallemantia iberica seed mucilage edible coating to improve the physicochemical, microbiological and sensory properties of lamb during refrigerated storage. Journal of Food Measurement and Characterization, 15(6), 5556-5571. doi:10.1007/s11694-021-01129-9
[16] Alizadeh Behbahani, B., Falah, F., Vasiee, A., & Tabatabaee Yazdi, F. (2021). Control of microbial growth and lipid oxidation in beef using a Lepidium perfoliatum seed mucilage edible coating incorporated with chicory essential oil. Food Science & Nutrition, 9(5), 2458-2467. doi:https://doi.org/10.1002/fsn3.2186
[17] Behbahani, B. A., Noshad, M., & Falah, F. (2019). Study of chemical structure, antimicrobial, cytotoxic and mechanism of action of Syzgium aromaticum essential oil on foodborne pathogens. Potravinarstvo, 13(1).
[18] Mehrnia, M. A., Alizadeh Behbahani, B., Barzegar, H., & Tanavar, H. (2021). Sclerorhachis platyrachis essential oil: Antioxidant power, total phenolic and flavonoid content and its antimicrobial activity on some Gram-positive and Gram-negative bacteria “in vitro”. Journal of food science and technology (Iran), 18(112), 189-198.
[19] Sureshjani, M. H., Yazdi, F. T., Mortazavi, S. A., Behbahani, B. A., & Shahidi, F. (2014). Antimicrobial effects of Kelussia odoratissima extracts against food borne and food spoilage bacteria" in vitro. Journal of Paramedical Sciences, 5(2), 115-120.
[20] Barzegar, H., Alizadeh behbahani, B., & Noshad, M. (2021). Evaluation of total phenol and flavonoid contents, antioxidant and antimicrobial activity of Lawsonia inermis aqueous extract against some gram- positive and gram- negative bacteria. Mdrsjrns, 18(116) 327-335.
[21] Rahmati-Joneidabad, M., & Noshad, M. (2021). Antifungal effect of Satureja khuzestanica essential oil on Aspergillus niger, Botrytis cinerea, and Rhizopus stolonifer causing strawberry’s rot and mold. Journal of food science and technology (Iran), 18(115), 171-180.
[22] Diao, W.-R., Hu, Q.-P., Zhang, H., & Xu, J.-G. (2014). Chemical composition, antibacterial activity and mechanism of action of essential oil from seeds of fennel (Foeniculum vulgare Mill.). Food Control, 35(1), 109-116. doi:https://doi.org/10.1016/j.foodcont.2013.06.056.
[23] Telci, I., Demirtas, I., & Sahin, A. (2009). Variation in plant properties and essential oil composition of sweet fennel (Foeniculum vulgare Mill.) fruits during stages of maturity. Industrial Crops and Products, 30(1), 126-130. doi:https://doi.org/10.1016/j.indcrop.2009.02.010.
[24] Conforti, F., Statti, G., Uzunov, D., & Menichini, F. (2006). Comparative chemical composition and antioxidant activities of wild and cultivated Laurus nobilis L. leaves and Foeniculum vulgare subsp. piperitum (Ucria) coutinho seeds. Biological and Pharmaceutical Bulletin, 29(10), 2056-2064.
[25] Namazi, P., Barzegar, H., & Mehrnia, M. A. (2021). Evaluation of functional groups of bioactive compounds, antioxidant potential, total phenolic and total flavonoid content of red bell pepper extracts. Journal of food science and technology (Iran), 18(113), 301-311.
[26] El Ouariachi, E., Lahhit, N., Bouyanzer, A., Hammouti, B., Paolini, J., Majidi, L., . . . Costa, J. (2014). Chemical composition and antioxidant activity of essential oils and solvent extracts of Foeniculum vulgare Mill. from Morocco. J. Chem. Pharm. Res, 6(4), 743-748.
[27] Barzegar, H., Mehrnia, M. A., & Alizadeh Behbahani, B. (2018). Determination of the chemical composition, antioxidant activity and the antimicrobial effect of Heracleum Lasiopetalum on infection and food poisoning microorganisms. J Appl Microbiol Food Indust, 4(4), 15-28.
[28] Singh, G., Maurya, S., De Lampasona, M. P., & Catalan, C. (2006). Chemical constituents, antifungal and antioxidative potential of Foeniculum vulgare volatile oil and its acetone extract. Food control, 17(9), 745-752.
[29] Noshad, M., & Falah, F. (2019). Investigation of antimicrobial activity of Fennel essential oil on some pathogenic microorganisms causing infection and food poisoning and its interaction with kanamycin antibiotic. Journal of food science and technology (Iran), 16(91), 233-241.