تاثیر پیش تیمار اولتراسوند به همراه حلال‌های مختلف بر فعالیت آنتی‌اکسیدانی و مقدار ترکیبات فنلی و فلاونوئیدی عصاره استخراج شده از گیاه هوفاریقون (Hypericum perforatum L.)

نویسنده
گروه شیمی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران.
چکیده
در پژوهش حاضر تاثیر پیش تیمار اولتراسوند در توان 40 کیلو وات و در مدت زمان 20 دقیقه به همراه حلال‌های تک جزئی آب و اتانل و دو جزیی آب/اتانل به صورت 30% (آب):70% (اتانل) و 60% (آب):40% (اتانل)، بر بازدهی استخراج، مقدار کل ترکیبات فنولی و فلاوونوئیدی و همچنین میزان مهار رادیکال‌های آزاد عصاره استخراج‌شده از گیاه هوفاریقون مورد بررسی قرار گرفت. در ادامه به‌منظور تجزیه و تحلیل داده‌ها، از طرح کاملاً تصادفی و در سه تکرار استفاده شد و مقایسه میانگین­ها توسط آزمون چند دامنه­ای دانکن، در سطح احتمال α=1% و با استفاده از نرم‌افزار SPSS نسخه 14 انجام پذیرفت. طبق نتایج، بیشترین میزان استخراج (3/18 درصد)، مقادیر کل ترکیبات فنولی (mg GAE/g dw 295/0) و فلاوونوئیدی (mg QE/g dw 105/0) و میزان مهار رادیکال‌های آزاد (21/84 درصد) در نمونه‌های پیش تیمار شده با اولتراسوند به همراه حلال دو جزیی آب / اتانل ]30% (آب):70% (اتانل)[ و کمترین میزان استخراج (21/4 درصد)، مقادیر کل ترکیبات فنولی (mg GAE/g dw 051/0) و فلاوونوئیدی (mg QE/g dw 0115/0) و همچنین میزان مهار رادیکال‌های آزاد (53/47 درصد) در حلال تک جزئی آب (100%) اندازه‌گیری شدند.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Impact of ultrasound pretreatment with different solvents on the antioxidant activity, phenolic and flavonoid compounds of the St. John’s wort (Hypericum perforatum L.) extract

نویسنده English

Leila Yousefi
Department of Chemistry, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran.
چکیده English

In this study, the effect of ultrasound pretreatment (at 40 kW power, for 20 min) with single partial solvent including water and ethanol and two partial solvents containing water/ethanol in the form of 30% (water): 70% (ethanol) and 60% (water): 40% (ethanol), were considered on the extraction efficiency, total phenolic and flavonoid compounds, as well as the free radical inhibition rate of the St. John’s wort (Hypericum perforatum L.) extract. In order to analyze the data, a completely randomized design was used in three replications, and the comparison of means was done by Duncan's multi-range test, at the probability level of α=1%, and using SPSS 14. Based on the results, the highest amount of extraction efficiency (18.3 %), total phenolic (0.295 mg GAE/g dw) and flavonoid (0.105 mg QE/g dw) compounds and the inhibition rate of free radicals (84.21 %) was measured in the samples pre-treated with ultrasound along with two partial solvents [30% (water): 70% (ethanol)] and the lowest amount of extraction efficiency (4.21%), total phenolic (0.051mg GAE/g dw) and flavonoid (0.0115 mg QE/g dw) compounds as well as free radical inhibition rate (47.53 %) was specified in water (100 %) solvent.

کلیدواژه‌ها English

St. John’s wort
Hypericum perforatum L
Ultrasound
Extraction
phenolic compounds
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