اثرات ضد دیابتیک شیر پروبیوتیک حاوی لاکتوباسیلوس رامنوسوس IBRC-M 10754 در مدل حیوانی موش ویستار و بررسی زنده مانی باکتری در شیر در شرایط سرد

نویسندگان
ماهرخ مظفری 1
مژگان امتیازجو 2
امیر علی انوار 3
1 دانش آموخته مهندسی صنایع غذایی دانشگاه آزاد اسلامی واحد علوم و تحقیقات
2 دانشگاه آزاد اسلامی واحد تهران شمال
3 دانشکده علوم و فنون دریایی دانشگاه آزاد اسلامی واحد تهران شمال
10.22034/FSCT.20.138.16
چکیده
درپژوهش حاضر بررسی زنده مانی لاکتوباسیلوس رامنوسوس IBRC-M 10754 در شیر بعنوان پروبیوتیک و ارزیابی اثرات ضد التهابی آن مورد بررسی قرار گرفته است . پس از کشت باکتری در محیط MRS , و افزودن آن به شیر ، تیمارهایی حاوی cfu/ml 109، 108و 107 از شیر پروبیوتیک تهیه شد. زنده مانی باکتری در بازه زمانی 5 روز بررسی گردید. سپس با گاواژ شیر حاوی پروبیوتیک به موش ها ی نر نژاد Balb/C که با استفاده از استرپتوزتوسین به دیابت مبتلا شده بودند، در مقابل موش های شاهد واثرات ضد دیابتیک شیر حاوی لاکتوباسیلوس رامنوسوس با سنجش میزان گلوکز خون و وزن در آنها بررسی شد.نتایج نشان داد بالاترین تعداد باکتری‌های زنده دردمای 4 درجه سانتی گراد شیر مربوط به روز اول نگهداری بوده و از روز دوم تا انتهای دوره نگهداری تعداد آن­ها اختلاف معناداری نداشت. در مجموع با گذشت زمان جمعیت باکتری های در شیر به صورت معنی داریp≤0/05) )کاهش یافت. افزودن مایه تلقیح اولیه cfu/ml 109نشان داد که در پایان روز پنجم جمعیت باکتری در شیر به میزان لازم در شیر پروبیوتیک تا پایان دوره سردخانه گذاری بود. بررسی وزن موش‌ها بیانگر آن است که وزن موش های شاهد در طول دوره نگهداری افزایش ولی موش‌های دیابتی در این بازه زمانی ابتلا کاهش یافت . شیر به همراه باکتری‌ لاکتوباسیلوس تلقیح شده در موش‌های دیابتی شده در دوز­های مختلف تلقیح منجر به کاهش معنادار (05/0>P) قند خون در دوره نگهداری 21 روزه آنها شدند. این کاهش از روز چهاردهم تغذیه با شیر پروبیوتیک مشهودتر است.
کلیدواژه‌ها
پروبیوتیک
شیر
ضد دیابت
لاکتوباسیلوس رامنوسوس

موضوعات

عنوان مقاله English

Anti-diabetic effect of probiotic milk containing Lactobacillus rhamnosus IBRC-M10754 in animal model of wistar rat and survive it in cold storage conditions

نویسندگان English

Mahrokh Mozafari 1
MOZHGAN EMTYAZJOO 2
Amir ali Anvar 3
1 Department of Food Science and Technology, Science and Research Branch, Tehran- Iran
2 Azad Islamic university North Tehran Branch
3 Marine science and technology ,Islamic Azad university north Tehran branch
چکیده English

The aim of the present study was to evaluate the viability of Lactobacillus rhamnosus IBRC-M 10754 in milk as a probiotic in cold storage and to evaluate its anti-diabetic effects. After bacterial culture in MRS medium and addition to milk, treatments containing 107, 108 and 109 cfu / ml of probiotic milk were prepared. Bacterial viability was performed for 5 days by sequential dilution and extensive culture in three replicates. Then, gavage containing probiotic-containing milk to Balb/C male rats that were infected with Streptozotocin was compared with anti-diabetic rats fed L. rhamnosus milk by measuring their blood glucose and weight. The results showed that the highest number of live bacteria in milk at 4Cº ,belonged to the first day of storage and there was no significant difference from the second day until the end of storage period. Overall, the bacterial population in milk decreased significantly (p≤0.05) over time. Addition of 109 cfu/ml primary inoculum showed that at the end of the fifth day the bacterial population in the milk was as high as that in the probiotic milk by the end of the cold storage. Mice weighing showed that the weight of control rats increased during the storage period, but diabetic rats decreased during this time. Milk with Lactobacillus inoculated in diabetic rats at different doses of inoculation resulted in a significant (P <0.05) decrease in blood glucose during their 21-day storage period. The decline of the fourteenth day of feeding probiotics is more evident.

کلیدواژه‌ها English

Probiotic
Milk
Anti-diabetic
Lactobacillus rhamnosus
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