تأثیر وزن مولکولی کیتوزان و نوع نرم‌کننده در پوشش خوراکی بر شاخص‌های کیفیت داخلی و ریخت‌شناسی پوسته تخم‌مرغ

نویسندگان
1 گروه کشاورزی، واحد سوادکوه، دانشگاه آزاد اسلامی، سوادکوه، ایران
2 گروه کشاورزیف واحد سوادکوهف دانشگاه آزاد اسلامی، سوادکوه، ایران
چکیده
به­ منظور بررسی تأثیر وزن مولکولی کیتوزان و نوع نرم­ کننده مورد استفاده در ترکیب پوشش خوراکی بر شاخص ­های کیفیت داخلی و ریخت­ شناسی پوسته تخم ­مرغ، آزمایشی با 125 عدد تخم­ مرغ در قالب طرح کاملاً تصادفی با 5 تیمار شامل تخم­ مرغ­ های فاقد پوشش (شاهد)، دارای پوشش­های مبتنی بر کیتوزان­های با وزن مولکولی زیاد (450 کیلو دالتون) یا وزن مولکولی کم (65 کیلو دالتون) و بکارگیری نرم ­کننده­ های گلیسرول یا سوربیتول و 3 تکرار اجرا شد. پوشش­ دهی به ­روش غوطه ­وری انجام شد و تخم­ مرغ­ها به­ مدت 4 هفته در دمای محیط نگهداری شدند. فراسنجه های کیفیت داخلی تخم­ مرغ شامل افت وزنی، واحد هاو، اندیس زرده و pH سفیده، بطور هفتگی ارزیابی شدند. در پایان دوره نگهداری، تصاویر میکروسکوپ الکترونی روبشی جهت ریخت­ شناسی پوسته تهیه شد. نتایج نشان داد پوشش خوراکی بر پایه کیتوزان به ­طور معنی­داری (P<0.05) موجب بهبود تمام شاخص­های مورد ارزیابی شد. در پایان هفته چهارم نگهداری، در این میان، کمترین مقادیر درصد افت وزنی (90/0 ± 01/7) و pH سفیده تخم­ مرغ­ (15/0 ± 90/8) و بیشترین مقادیر واحد هاو (23/3 ± 80/63) و اندیس زرده (06/0 ± 34/0) در تخم­ مرغ­های حاوی پوشش کیتوزان وزن مولکولی زیاد سوربیتول دیده شد (P<0.05). در تصاویر میکروسکوپ الکترونی روبشی نیز ترک و ریزحفرات کمتر و یکنواختی و پیوستگی بیشتری در پوسته خارجی تخم­ مرغ­ها در گروه مذکور دیده شد. لذا به­ منظور بهبود کیفی پوشش خوراکی بر پایه کیتوزان به ­عنوان یک بسته­ بندی زیست­ سازگار جهت افزایش زمان انبارمانی تخم­ مرغ، کیتوزان با وزن مولکولی بیشتر و نرم­ کننده سوربیتول توصیه می­شود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Effect of chitosan molecular weight and type of plasticizer in edible coating on internal quality and eggshell morphology

نویسندگان English

Aref sharifi 1
dariush khademi shurmasti 2
1 Dep. of Agriculture, Savadkooh branch, Islamic Azad University, Savadkooh, Iran
2 Dep. of Agriculture, Savadkooh branch, Islamic Azad Univaersity, Savadkooh, Iran
چکیده English

To investigate the effect of molecular weight of chitosan and the type of plasticizer used in the composition of edible coating on internal quality parameters and morphology of eggshell, an experiment with 125 eggs in a completely randomized design with 5 treatments included uncoated eggs (control), coated based on chitosan with high molecular weight (450 kDa) or low molecular weight (65 kDa) and the use of glycerol or sorbitol and 3 replicates was conducted. Coating was done by immersion method and the eggs were stored at ambient temperature for 4 weeks. The internal quality parameters of eggs including weight loss (WL), Haugh unit (HU), yolk index (YI) and albumen pH were evaluated weekly. At the end of the storage period, scanning electron microscope (SEM) micrograph were prepared for eggshell morphology. The results showed that the edible coating based on chitosan improved significantly (P<0.05) all the evaluated parameters. At the end of the week 4, among these, the lowest values of WL (7.01 ± 0.90) and pH of albumen (8.90 ± 0.15) and the highest values of HU (± 3.23) 63.80) and YI (0.34 ± 0.06) were seen in eggs containing high molecular weight chitosan-sorbitol coating (P<0.05). In the SEM micrograph, more integrated and less porous and fractured in the mentioned group. Therefore, in order to improve the quality of edible coating based on chitosan as a biocompatible packaging to increase the storage time of eggs, chitosan with higher molecular weight and sorbitol as plasticizer are recommended.

کلیدواژه‌ها English

Egg
Sorbitol
Chitosan
glycerol
shelf life
1- Xu, L., Zhang, H., Lv, X., Chi, Y., Wu, Y. and Shao, H. 2017. Internal quality of coated eggs with soy protein isolate and montmorillonite: Effects of storage conditions, International Journal of Food Properties, 20:8, 1921-1934, DOI: 10.1080/10942912.2016.1224896.
2- Kumari, S. and Rath, P.K. 2014. Extraction and characterization of chitin and chitosan from (Labeo rohit) fish scales. Procedia Materials Science, 6: 482–489.
3- No, H.K., Prinyawiwatkul, W. and Meyers, S.P. 2005. Comparison of shelf life of eggs coated with chitosan prepared under various deproteinization and demineralization times. Journal of Food Science, 70: S377–82.
4- Hajighasem Sharbatdar, H. and Khademi Shurmasti, D. 2022. The effect of bio-filler-reinforced chitosan coating with types of solvent on internal changes and outer eggshell morphology. SVU- International Journal of Veterinary Sciences, 5(2): 45-54.
5- Ehsan, M. and Khademi Shurmasti, D. 2021. Effect of washing and active nanocomposite coating of carboxymethyl cellulose-nanoclay containing marjoram extract (Origanum vulgare L) on egg quality during storage at ambient temperature. Iranian Journal of Food Science and Technology, 118(18): 107-118. (In Persian).
6- Peniche, C., Peniche, H. and Pérez, J. 2018. Chitosan based self-assembled nanoparticles in drug delivery. Polymers, 10: 235.
7- Zhong, Y., Zhuang, C., Gu, W. and Zhao, Y. 2019. Effect of molecular weight on the properties of chitosan films prepared using electrostatic spraying technique. Carbohydrate Polymers, 212: 197–205.
8- Suyatma, N. E., Tighzert, L., Copinet, A. and Coma, V. 2005. Effects of hydrophilic plasticizers on mechanical, thermal, and surface properties of chitosan films. Journal of Agricultural and Food Chemistry, 53(10): 3950-3957.
9- Vieira, M.G.A., da Silva, M.A., dos Santos, L.O. and Beppu, M.M. 2011. Natural-based plasticizers and biopolymer films: A review. European Polymer Journal, 47(3): 254-263.
10- Haq, M.A., Hasnain, A. and Azam, M. 2014. Characterization of edible gum cordia film: Effects of plasticizers. LWT-Food Science and Technology, 55(1): 163-169.
11- Wittaya, T. 2013. Influence of type and concentration of plasticizers on the properties of edible film from Mung bean proteins. KMITL Science and Technology Journal, 13(1): 51-58.
12- Kim, S.H., No, H.K., Prinyawiwatkul, W. 2007. Effect of molecular weight, type of chitosan, and chitosan solution pH on the shelf-Life and quality of coated eggs. Journal of Food Science, 72(1): S044–S048.
13- Park, Y.S., Yoo, I.J., Jeon, K.H., Kim, H.K., Chang, E.J. and Oh, H.I. 2003. Effects of various eggshell treatments on the egg quality during storage. Asian-Australasian Journal of Animal Science,16(8): 1224-1229.
14- Haugh, R.R. 1937. A new method for determining the quality of an egg. US Egg Poultry, 39: 27-49.
15- Funk, E.M. 1973. Egg science and technology. In: Egg Science and Technology. Stadelman, W.J. and Cotterill, O.J. (Eds.). AVI Publishing Company, Inc., Westport, CT.
16- Koga, O., Fujihara, N. and Yoshimura, Y. 1982. Scanning electron micrograph of surface structures of soft-shelled eggs laid by regularly laying hens. Poultry Science, 61:403-406.
17- Suresh, P.V., Raj, K.R., Nidheesh, T., Pal, G.K. and Sakhare, P.Z. 2015. Application of chitosan for improvement of quality and shelf life of table eggs under tropical room conditions. Journal of Food Science and Technology, 52: 6345–6354.
18- Yaghobi, N., Mirzadeh, H. and Vakili, H. 2013. Preparation of different molecular weight chitosans by controlling multistage deacetylation conditions and its effect on porosity of prepared scaffolds. Journal of Applied Research in Chemistry, 7(2):41-50. (In Persian)
19- Talebi, H., Ghasemi Ashenai, F. and Ashori, A. 2019. The effect of solvent and plasticizer on mechanical properties of chitosan-based biocomposites. Polymerization, 9(3):62-71. (In Persian).
20- Bourtoom, T. 2008. Plasticizer effect on the properties of biodegradable blend film from rice-chitosan. Songklanakarin Journal of Science and Technology, 30:149-165.
21- Kim, S.H., No, H.K. and Prinyawiwatkul, W. 2008. Plasticizer types and coating methods affect quality and shelf life of eggs coated with chitosan. Journal of Food Science, 73:111–117.
22- Dias, A.B., Muller, C.M., Larotonda, F.D. and Laurindo, J.B. 2010. Biodegradable films based on rice starch and rice flour. Journal of Cereal Science, 51: 213–219.
23- USDA. 2000. United States standards, grades, and weight classes for shell eggs. AMS 56.210.
24- Pires, P.G.S., Leuven, R.F.A., Franceschi, C.H., Machado, G.S., Pires, P.D.S., Moraes, P.O., Kindlein, L. and Andretta, I. 2020. Effects of rice protein coating enriched with essential oils on internal quality and shelf life of eggs during room temperature storage. Poultry Science, 99:604-611.
25- Soares, R.D.A., Borges, S.V., Dias, M.V., Piccoli, R.H., Fassani, E.J. and Cunha da Silva, E.M. 2021. Impact of whey protein isolate/sodium monmorillonite/sodium metabisulfite coating on the shelf life of fresh eggs during storage. LWT- Food Science and Technology, 139:110611.
26- Rachtanapun, P., Homsaard, N., Kodsangma, A., Phongthai, S., Leksawasdi, N., Phimolsiripol, Y., Seesuriyachan, P., Chaiyaso, T., et al. 2022. Effects of storage temperature on the quality of eggs coated by cassava starch blended with carboxymethyl cellulose and paraffin wax. Poultry Science, 101:101509.
27- Xu, D., Wang, J., Ren, D., Wu, X. 2018. Effects of chitosan coating structure and changes during storage on their egg preservation performance. Coatings, 8: 317. Doi:10.3390/coatings8090317.
28- Wantat, A., Rojsitthisak, P. and Seraypheap, K. 2021. Inhibitory effects of high molecular weight chitosan coating on ‘Hom Thong’ banana fruit softening. Food Packaging and Shelf Life, 29(54):100731.
29- Pires, P.G da silva, Franceschi, C.H., Bavaresco, C., Leuven, A.F.R. and Andretta, I. 2020. Plasticizer types and whey protein coatings on internal quality and shelf life of eggs stored for 42 days. Food Science and Technology, 78(suppl. 1): e20200271.