بررسی ترکیبات شیمیایی و ضدمیکروبی عصاره گیاه پونه (Mentha pulegium) بومی مشگین شهر و نمین

نویسندگان
سیده معصومه هاشمی نیا 1
منیژه جمشیدی 2
1 استادیار، گروه زراعت و اصلاح نباتات، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران.
2 استادیار، گروه گیاه پزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
10.22034/FSCT.19.130.97
چکیده
در این پژوهش، ترکیبات شیمیایی عصاره اِن-هگزانی گیاه پونه بومی مناطق مشگین شهر و نمین و همچنین اثر ضدمیکروبی آن بر باکتری­های سودوموناس آئروجینوزا (ATCC 27853) و باسیلوس سرئوس (ATCC 11778) با دو روش تکنیک رقت در لوله (ارزیابی حداقل غلظت مهارکنندگی و حداقل غلظت باکتری­کشی) و روش دﻳﺴﻚ دیفیوژن مورد بررسی قرار گرفت. طبق نتایج، مهمترین ترکیبات تشکیل دهنده عصاره­های مذکور سیس-پیپریتنون اپوکسید، پیپریتنون اکسید و پولگون شناسایی شدند. علاوه بر این حداقل غلظت مهارکنندگی و حداقل غلظت باکتری­کشی عصاره­های اِن-هگزانی گیاه پونه مربوط به مناطق مختلف علیه باکتری سودوموناس آئروجینوزا مشابه هم و به میزان 20 میلی­گرم بر میلی­لیتر گزارش گردید. از سوی دیگر حداقل غلظت مهارکنندگی و حداقل غلظت باکتری­کشی عصاره اِن-هگزانی گیاه پونه مشگین شهر علیه باکتری باسیلوس سرئوس به ترتیب برابر 5 و 10 میکروگرم بر میلی­لیتر در حالی‌که علیه باکتری باسیلوس سرئوس مشابه هم و به میزان 10 میلی­گرم بر میلی­لیتر اندازه‌گیری گردیدند. همچنین نتایج ارزیابی آزمون دﻳﺴﻚ دیفیوژن نشان داد که هاله عدم رشد عصاره اِن-هگزانی نمونه‌های به دست آمده از منطقه مشگین شهر علیه هر دو باکتری بیشتر از هاله عدم رشد نمونه‌های به دست آمده از منطقه نمین بودند.
کلیدواژه‌ها
باسیلوس سرئوس
پونه
سودوموناس آئروجینوزا
عصاره
ضدباکتریایی

موضوعات

عنوان مقاله English

Investigation of chemical and antimicrobial compounds of native Mentha pulegium extract of Meshginshahr and Namin

نویسندگان English

Seyedeh Masoomeh Hasheminia 1
Manizheh Jamshidi 2
1 Assistant Professor, Department of Agronomy, College of Agriculture, Roudehen Branch, Islamic Azad University, Roudehen, Iran.
2 Assistant Professor, Department of Plant Protection, College of Agriculture, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
چکیده English

The chemical constituents of n-hexane extract of Mentha arvensis (wild mint) native to Meshginshahr and Namin region, and the antimicrobial effect of the extract against Pseudomonas aeruginosa (ATCC 27853) and Bacillus cereus (ATCC 11778) were studied in this research using broth and agar dilution methods (assessment of minimum inhibitory concentration, MIC, and minimum bactericidal concentration, MBC) and disk diffusion method. The results showed that cis-piperitone epoxide, piperitenone oxide and pulegone were the main components of the extracts. In addition, the MICs and MBCs of the n-hexane extracts of the wild mint in the various regions against P. aeruginosa were similar (20 mg/mL). Moreover, the MIC and MBC of the n-hexane extracts of the wild mint in Meshginshahr against B. cereus were 5 and 10 μg/mL, respectively, whereas the corresponding values against B. cereus were similar (10 mg/mL). Furthermore, the results of the disk diffusion test interpretation revealed that the inhibition zone diameters (IZDs) of the n-hexane extract in the samples obtained from Meshginshahr against both bacterial species were larger than those of the samples taken from Namin.

کلیدواژه‌ها English

Bacillus cereus
Mentha pulegium
Pseudomonas aeruginosa
Extract
Antibacterial
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