غربالگری جدایه های باکتری های اسید لاکتیک از فرآورده‌های لبنی سنتی خراسان به منظور ارزیابی تولید فولات خارج سلولی

نویسندگان
فاطمه حسینی
محمدباقر حبیبی نجفی
محمدرضا عدالتیان دوم
دانشگاه فردوسی مشهد
10.22034/FSCT.19.130.37
چکیده
فولات واژه ای است که برای مجموع ترکیبات دارای فعالیت زیستی مشابه به کار می­رود. فولات (پلی گلوتامات ها)، تمام اشکال ویتامین های این خانواده را به طور طبیعی شامل می شود. به عبارت دیگر اسید فولیک شکلی از فولات است که به فرم اکسیده می­باشد، این ترکیب پایدار بوده و به راحتی توسط انسان قابل جذب می­باشد (مونوگلوتامات).[1]. در این مطالعه میزان تولید فولات خارج سلولی(کواتروفولات) بررسی شد بنابراین از روش سنجش میکروبی به عنوان روش اصلی استفاده شد.این روش متکی بر رشد باکتریایی و سنجش کدورت لاکتوباسیلوس رامنوسوس (ATCC 7469) است که به عنوان ارگانیسم سنجش استفاده شد.[2,3]. در این پژوهش 47 سویه باکتری ایزوله شده از فرآورده های لبنی سنتی به ویژه ماست محلی مناطق عشایر خراسان مورد مطالعه و بررسی قرار گرفت. نتایج بدست آمده از این ارزیابی نشان داد که دو سویه شماره(25و26) از سویه­های لاکتوباسیوس دلبروکی زیر گونه بولگاریکوس تولید قابل توجهی از غلظت بالای فولات (μg/mL133.23و μg/mL102.34) را داشتند، همچنین سویه K73 از سویه­های استرپتوکوکوس ترموفیلوس، سویه شماره 73 از سویه­های لاکتوباسیلوس دلبروکی زیرگونه لاکتیس و سویه شماره 87 از سویه­های لاکتوباسیلوس هلوتیکوس به ترتیب (μg/mL 90.29، μg/mL39.71 ، μg/mL 17.94) نیز تولید مقادیر بالایی از غلظت کواتروفولات را داشتند. با توجه به این پژوهش مشخص شد جدایه­های مورد بررسی توانایی بالقوه­ای در تولید فولات خارج سلولی دارند. همچنین روش سنجش میکروبی بعنوان روشی کارآمد از قابلیت لازم برای اندازه گیری فولات و مشتقات آن برخوردار است.، .
کلیدواژه‌ها
فولات خارج سلولی
سنجش میکروبی
استرپتوکوکوس ترموفیلوس
لاکتوباسیلوس دلبروکی زیرگونه بولگاریکوس
لاکتوباسیلوس دلبروکی زیرگونه لاکتیس
لاکتوباسیلوس هلوتیکوس

موضوعات

عنوان مقاله English

Screening of lactic acid bacteria isolated from traditional dairy products of Khorasan regarding extracellular folate production

نویسندگان English

ّFatemeh Hossini
Mohammad B. Habibi Najafi
Mohammad Reza Edalatiane
Ferdowsi University of Mashhad
چکیده English

Folate is a term used for a set of compounds with similar biological activities. Folate (polyglutamates) naturally contains all forms of vitamins in this family. In other words, folic acid is a form of folate that is oxidized, this compound is stable and can be absorbed by humans (monoglutamate)[1]. There are several methods for measuring folate, but due to the fact that in this study, the production of extracellular folate (Quatrofolate) was examined, so the microbial assay method was used as the main method. Lactobacillus. Rhamnosus (ATCC7469), which is used as a sensing organism. [2,3] This method can also measure all forms of folate. In this study, 47 medical strains isolated from traditional dairy products were Special Khorasan. traditional yogurt (12 strains of Streptococcus.thermophilus and 15 strains of Lactobacillus delbrueckii subsp. Bulgaricus, 13 strains of Lactobacillus delbrueckii subsp. Lactis, 3 strains of Lactobacillus helveticus) were studied. The results of this evaluation showed that both strains (25 and 26) of Lactobacillus delbrueckii subsp. Bulgaricus produced significant high folate concentrations (133.23 μg / mL and 102.34 μg / mL), as well as K73 strain of Streptococcus.thermophilus, strain 73 from the strains of Lactobacillus delbrueckii subsp. Lactis and strain No.87 from the three strains of Lactobacillus helveticus (90.29 μg / mL, μg / mL39.71, μg / mL 17.94), were also able to produce high amounts of quatrofolate. According to this study, it was found that the examined previously isolated strains have a potential ability to produce extracellular folate, and the microbial assay method for measuring a sensitive vitamin such as folate in all its forms is considered an efficient and accurate method.

کلیدواژه‌ها English

Extracellular folate
Microbial assay
Streptococcus thermophilus
Lactobacillus delbroki subspecies bulgaricus
Lactobacillus delbrucci subspecies lactis
Lactobacillus helveticus
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