ارزیابی فنول و فلاونوئید کل، پتانسیل آنتی‌اکسیدانی و فعالیت ضدمیکروبی عصاره مریم گلی تفتانی بر تعدادی از باکتری‌های گرم مثبت و گرم منفی در شرایط آزمایشگاهی

نویسندگان
حسن برزگر 1
حسین جوینده 2
1 دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 استاد، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.22034/FSCT.19.125.195
چکیده
بیماری­های ناشی از مصرف نگهدارنده­های شیمیایی تمایل به استفاده از ترکیبات ضد میکروبی و آنتی‌اکسیدانی طبیعی را افزایش داده است. در این پژوهش، عصاره گیاه درمانی مریم گلی تفتانی با استفاده از حلال اتانول استخراج گردید. میزان فنول و فلاونوئید کل عصاره اتانولی به ترتیب برابر با 14/36 میلی‏گرم گالیک اسید در گرم عصاره و 13/17 میلی‌گرم کوئرستین در گرم عصاره به دست آمد که نشان­دهنده خاصیت آنتی‌اکسیدانی بالای این عصاره بود. به گونه­ای که میزان فعالیت آنتی‌اکسیدانی عصاره بر اساس IC50 در روش مهار رادیکال آزاد DPPH و مهار رادیکال آزاد ABTS به ترتیب 32/75 و 60/66 میکرو­گرم در میلی­لیتر به دست آمد. ارزیابی خاصیت ضد میکروبی عصاره اتانولی گیاه مریم گلی تفتانی به روش­های دیسک دیفیوژن آگار، انتشار چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی نشان داد که اگرچه اثر بخشی عصاره بر باکتری‌های گرم مثبت بیشتر از باکتری‌های گرم منفی است، بااین‌حال این گیاه می­تواند به عنوان یک نگهدارنده طبیعی در صنایع مختلف مورد استفاده قرار گیرد.
کلیدواژه‌ها
گیاه مریم گلی تفتانی
عصاره اتانولی
خاصیت ضد میکروبی
فعالیت آنتی‌اکسیدانی

موضوعات

عنوان مقاله English

Evaluation of total phenol and flavonoids, antioxidant potential and antimicrobial activity of sage extract on some Gram-positive and Gram-negative bacteria “in vitro”

نویسندگان English

Hassan Barzegar 1
Hossein Jooyandeh 2
1 1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2 Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

Diseases caused by the use of chemical preservatives have increased the tendency to use natural antimicrobial and antioxidant compounds. In this study, the herbal extract of Salvia rhytidea Benth was extracted using ethanol solvent. The content of total phenols and flavonoids in the ethanolic extract were found to be 36.14 mg GAE/g and 17.13 mg QE/g, respectively, indicating the high antioxidant properties of the extract. The antioxidant activity of the extract based on IC50 in DPPH free radical scavenging and ABTS free radical scavenging was 75.32 and 66.60 mg/ml, respectively. Evaluation of antimicrobial properties of ethanolic extract of S. rhytidea by disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration showed that although the extract was more effective against Gram-positive bacteria than Gram-positive bacteria, the plant can be used as a natural preservative in various industries.

کلیدواژه‌ها English

Salvia rhytidea Benth
Ethanolic extract
Antimicrobial properties
Antioxidant activity
[1] Saravanan, A., Senthil Kumar, P., Hemavathy, R. V., Jeevanatham, S., Kamalesh, R., Sneha, S. and Yaashikaa, P. R. 2021. Methods of detection of food-borne pathogens: a review, Environmental Chemistry Letters. 19 (1):189-207.
[2] Namazi, P., Barzegar, H., Alizadeh Behbahani, and Mehrnia, M. A. 2021. Evaluation of functional groups of bioactive compounds, antioxidant potential, total phenolic and total flavonoid content of red bell pepper extracts. [in Fa.]. Iranian Journal of Food Science and Technology. 113 (18): 301- 311.
[3] Alizadeh Behbahani, B., Noshad, M., and Falah, F. 2019. Study of Chemical Structure, antimicrobila, cytotoxic and mechanism of action of syzygium aromaticum essential oil on foodborne pathogenes. Potravinarstvo. 13 (1): 875-883.
[4] Heredia, N. and García, S. 2018. Animals as sources of food-borne pathogens: A review, Animal nutrition. 4(3): 250-255.
[5] Barzegar, H., Alizadeh Behbahani, B. and Mehrnia, M. A. 2020. Quality retention and shelf life extension of fresh beef using Lepidium sativum seed mucilage-based edible coating containing Heracleum lasiopetalum essential oil: an experimental and modeling study. Food Science and Biotechnology. vol. 29, no. 5, pp. 717-728, 2020.
[6] Nychas, G. 1995. Natural antimicrobials from plants. in New methods of food preservation: Chapman and Hall, Glasgow, pp. 58-89.
[7] Singh, N., Singh, R., Bhunia, A. and Stroshine, R. 2002. Efficacy of chlorine dioxide, ozone, and thyme essential oil or a sequential washing in killing Escherichia coli O157: H7 on lettuce and baby carrots. LWT-Food Science and Technology. 35(8): 720-729.
[8] Rowshan, V., Khoi, M. K. and Javidnia, K. 2010. Effects of salicylic acid on quality and quantity of essential oil components in Salvia macrosiphon. Journal of Biological & Environmental Sciences. 4(11): 77-82.
[9] Zahabi, Z. F., Sharififar, F., Almani, P. G. N. and Salari, S. 2020. Antifungal activities of different fractions of Salvia rhytidea Benth as a valuable medicinal plant against different Candida species in Kerman province (Southeast Iran). Gene Reports, 19: https://doi.org/10.1016/j.genrep.2020.100624.
[10] Azizian-Shermeh, O. and Hassanabadi, A. 2021. Variation on biological activities and total phenol and flavonoids contents in different extracts from Salvia rhytidea Bent. collected from natural habitats of Kerman and Sistan and Baluchestan provinces. Ecophysiology & Phytochemistry of Medicinal and Aromatic Plants.9(1): 27-41.
[11] Azizian Shermeh, O., Taherizadeh, M., Valizadeh, M. and Qasemi, A. 2018. Antimicrobial and Antioxidant Activities and Determining Phenolic and Flavonoid Contents of the Extracts of Five Species from Different Families of the Medicinal Plants Grown in Sistan and Baluchestan Province. Journal of Advanced Biomedical Sciences. 7(4): 465-479.
[12] Azizian-Shermeh, O., Mollashahi, E. and Taherizadeh, M. 2020. Phytosynthesis of stable silver nanoparticles using aqueous extract of Salvia rhytidea Benth. and comparison of its antimicrobial activities with natural plant extract. Ecophytochemistry journal of Medicinal Plants. 8(2): 89-11.
[13] Nosratollahi, K., Barzegar, H., Jooyandeh, H. and Ghorbani, M. R. 2018. Effect of savory (Satureja hortensis) extract on the quality and shelflife of raw chicken meat stored at refrigerator. [in Fa.]. Journal of Food science and Technology. 82 (15): 167-176.
[14] Barzegar, H., Alizadeh Behbahani, B. and Noshad, M. 2021. Evaluation of total phenol and flavonoid contents, antioxidant and antimicrobial activity of Lawsonia inermis aqueous extract against
some Gram- positive and Gram- negative bacteria. [in Fa.]. Iranian Journal of Food Science and Technology. 16 (18): 327-335.
[15] Alizadeh Behbahani, B. and Fooladi, A. A. I. 2018. Evaluation of phytochemical analysis and antimicrobial activities Allium essential oil against the growth of some microbial pathogens. Microbial pathogenesis.114: 299-303.
[16] Haghjoo, L., Barzegar, H. and Jooyandeh, H. 2020. The effect of different drying methods on chemical composition and antioxidant properties of methanolic extract of olive leaf. [in Fa.]. Journal of Food Science and Technology. 97 (16): 149-159.
[17] Shahidi, F., Tbabatabaie, F., Roshanak, S., Alizadeh Behbahani, B., , Vasiee, A. and Norouzi, N. 2019. Antimicrobial activity of Taraxacum pseudocalocephalum leaves extract on pathogenic microorganisms and comparison with common therapeutic antibiotics in vitro. Iranian Journal of Infectious diseases and Tropical Medicine. 23 (83): 37-46.
[18] Tanavar, H., Barzegar, H., Alizade Behbahani, B. and Mehrnia, M. A. 2020. Evaluation of the antimicrobial activity of Mentha pulegium essential oil on some foodborne pathogens and its interaction with gentamicin and chloramphenicol in vitro. Food Science and Technology. 16(97): 77-87.
[19] Tanavar, H., Barzegar, H., Alizadeh Behbahani, B. and Mehrnia, M. A. 2020. Mentha pulegium essential oil: chemical composition, total phenolic and its cytotoxicity on cell line HT29. Iranian Food Science and Technology Research Journal.16(5): 643-653.
[20] Mazarei, A. and Fahmideh, L. 2018. Comparative evaluation of phenolic and flavonoids compounds, antioxidant and antibacterial activity of methanol extract of Artemisia annua L., Thymus vulgaris L., Matricaria chamomilla L., Salvia officinalis L. and Pistacia atlantica var mutica. Eco-phytochemical Journal of Medicinal Plants. 6(3):78-95.
[21] Alizadeh Behbahani, B. and Shahidi, F. 2019. Melissa officinalis essential oil: Chemical compositions, antioxidant potential, total phenolic content and antimicrobial activity. Nutrition and Food Sciences Research. 6(1): 17-25.
[22] Noshad, M. and Alizadeh behbahani, B. 2021. Evaluation of the effect of aqueous and ethanolic extraction methods on the antioxidant and antimicrobial characteristics of Lippia citriodora extract. Journal of food science and technology. 18 (118): 273-283.
[23] Alizade Behbahani, B. Tabatabaei Yazdi, F., Heidari Sureshjani, M., Mortazavi, A. and Tabatabaei Yazdi, F. 2014. Antimicrobial effect of the aqueous and ethanolic Satureja bachtiarica extracts “in vitro”. Iranian Journal of Infectious Diseases and Tropical Medicine. 19(64): 13-19.
[24] Saeedi, M., Yeganegi, M., Alizadeh Behbahani, B., Vasiee, A. and Tabatabaie, F. 2017. Antimicrobial effects of leek (Allium ampeloprasum l. Subsp. Iranicum) extract on some food-borne pathogens in vitro. Iranian Journal of Food Science and Technology. 14(68): 73-82.
[25] Salimpour, F., Mazooji, A., Mazhar, F. and Barzin, G. 2014. Comparative study of antibacterial properties of four species of Salvia L. as a medicinal plant. PAJOUHESH DAR PEZESHKI. 37(4): 205-210.
[26] Sookto, T., Srithavaj, T., Thaweboon, S., Thaweboon, B. and Shrestha, B. 2013. In vitro effects of Salvia officinalis L. essential oil on Candida albicans. Asian Pacific Journal of Tropical Biomedicine. 3(5): 376-380.
[27] Garcia, C. S. C. et al. 2012. Assessment of Salvia officinalis L. hydroalcoholic extract for possible use in cosmetic formulation as inhibitor of pathogens in the skin. Revista de Ciências Farmacêuticas Básica e Aplicada. 33(4): 509-514.