اثر پرتوتابی و نگهداری در غلظت‌های مختلف آب نمک و سرکه بر کیفیت و ماندگاری قارچ ترافل بیابانی (Terfezia claveryi)

نویسندگان
1 دانشجوی دکتری تخصصی علوم و صنایع غذایی، واحد ممقان، دانشگاه آزاد اسلامی، ممقان، ایران
2 استاد گروه علوم و صنایع غذایی، دانشکدۀ کشاورزی، دانشگاه تبریز، تبریز، ایران
3 دانشیار گروه بهداشت مواد غذایی،دانشکده دامپزشکی، علوم پزشکی تبریز، دانشگاه آزاد اسلامی، تبریز، ایران
4 دانشیار، گروه علوم و صنایع غذایی، دانشکده تغذیه و علوم غذایی، دانشگاه علوم پزشکی و خدمات بهداشتی - درمانی تبریز،تبریز، ایران
چکیده
در این پژوهش، به­منظور افزایش عمر ماندگاری و کیفیت قارچ ترافل بیابانی، قارچ­ها با پرتو فرابنفش ((UVC در طول موج 254 نانومتر با شدت mW cm−12/2 در سه زمان مختلف صفر (کنترل)، 10 و20 دقیقه و همچنین غوطه­وری در محلول کلرید سدیم با چهار غلظت 16،13،10،7 درصد و سرکه 5 درصد (وزنی/وزنی) تیمار شدند. سپس، قارچ­ها بمدت 160 روز در دمای C° 4 نگهداری و به فاصله زمانی 40 روز نمونه برداری انجام شد. ویژگی­های درصد کاهش وزن، سفتی بافت ، ترکیبات فنلی، ویتامین C و بار میکروبی ارزیابی شد. نتایج نشان داد که سفتی بافت (N 83/4) در قارچ­های UV-C (10 دقیقه) غوطه­ور درسرکه نسبت به سایر تیمارها بیشتر بود. همچنین پرتوتابی اثر معنی­داری در جلوگیری از کاهش وزن قارچ­ها داشت و کمترین و بیشترین میزان کاهش وزن به­ترتیب مربوط به تیمار UV-C (10 دقیقه) غوطه ور در سرکه و تیمار کنترل غوطه­ور در آب نمک 16٪ بود. ارزیابی ترکیبات فنلی به روش HPLC نشان داد که پرتوتابی اثر معنی­داری بر افزایش ترکیبات فنلی در قارچ­ها داشت با وجود اینکه مقدار این ترکیبات با گذشت زمان دچار کاهش معنی­دار شد. مقدار ترکیبات فنلی در تیمارهای UV-C (10 دقیقه) غوطه­ور در سرکه و کلرید سدیم 7٪ به­ترتیب نسبت به سایر تیمارها بیشتر بود. پرتوتابی همچنین اثر معنی­داری بر کاهش بار میکروبی قارچ­ها داشت و افزایش شدت پرتودهی موجب افزایش مهار رشد میکروبی شد. تابش پرتو UVC موجب کاهش ویتامین C تا 78٪ در نمونه­های تیمارشده گردید. غلظت­های بالای آب­نمک باعث دهیدراسیون بافت قارچ و افزایش افت وزن و کاهش ترکیبات فنلی و مقدار ویتامین C در طی نگهداری گردید. در کل، موثرترین تیمار جهت حفظ ویژگی­های فیزیکی و کیفی ترافل، UV-C (10 دقیقه) و غوطه‌وری در سرکه در طی 160 روز انبارداری بیان شد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Effect of UV-C irradiation and storage at the different concentration of brine and vinegar on the quality and shelf life of desert truffle (Terfezia claveryi)

نویسندگان English

Bahrareh Daei 1
Sodeif Azadmard-Damirchi 2
Afshin Javadi 3
Mohammad Ali Torbati 4
1 Department of Food Science and Technology, Mamaghan Branch, Islamic Azad University, Mamaghan, Iran
2 Professor, Department of Food Science, College of Agriculture, University of Tabriz, Tabriz 3392032, Iran
3 Associate Professor, Department of Food Hygiene, Faculty of Veterinary, Tabriz Medical Science, Islamic Azad University, Tabriz, Iran.
4 Associate Professor, Department of Food Science and Technology, Faculty of Nutrition and Food Science, Tabriz University of Medical Sciences
چکیده English

The present study was carried out to determine the effects of UV irradiation on postharvest and quality of desert truffle. in order to increase the shelf life of desert truffles, truffles were irradiated with UV-C (2.2 mW cm -1) at three different times, (control), 10 and 20 minutes and also storage in 7%, 10%, 13% 16% (w/w) NaCl and 5% vinegar solution (w/w). After applying the desired treatments, truffles were stored at 4 ° C for 160 days and sampling was performed at 40-day intervals. Weight loss percentage, tissue firmness, phenolic compounds, vitamin C content and microbial load were evaluated. Tissue firmness was higher at the truffles that were exposed to radiation for 10 minutes than other treatments. Also, radiation had a significant effect on preventing weight loss of truffles. The lowest weight loss was related to 10 minutes of radiation treatment and also, the highest amount of weight loss was related to control treatment. Radiation at higher doses does not further improve tissue quality parameters and causes skin necrosis and damage to truffle tissue. Evaluation of truffles phenolic compounds such as caffeic acid, ferulic acid, vanillic acid, coumaric acid, cinnamic acid and catechin was measured by HPLC. Evaluation shows that radiation had a significant effect on the increase of phenolic compounds in truffles, however the amount of these compounds decreased significantly over time. Irradiation also had a significant effect on reducing the microbial load and irradiated truffle had the lowest amount of microbial growth. However, the most effective treatment to maintain tissue quality characteristics and prevent weight loss and inhibit bacterial spoilage, UV-C (10 minutes) and immersion in vinegar and Storage at 4 ° C compared to other treatments was expressed during 160 days of storage.

کلیدواژه‌ها English

UV-c irradiation
Storage life
Truffle
NaCl
Vinegar
]1] Khabar, L., 2014. Mediterranean Basin: North Africa. In Desert Truffles. Springer, Berlin, Heidelberg. pp: 143-158.
]2] Čejka, T., Trnka, M., Krusic, P.J., Stobbe, U., Oliach, D., Václavík, T., Tegel, W. and Büntgen, U., 2020. Predicted climate change will increase the truffle cultivation potential in central Europe. Scientific reports, 10(1) :1-10.
]3] Zambonelli, A., Donnini, D., Rana, G.L., Fascetti, S., Benucci, G.M.N., Iotti, M., Morte, A., Khabar, L., Bawadekji, A., Piattoni, F. and Compagno, R., 2014. Hypogeous fungi in Mediterranean maquis, arid and semi-arid forests. Plant Biosystems-An International Journal Dealing With all Aspects of Plant Biology, 148(2):392-401.
]4] Alirezalu, K., Azadmard-Damirchi, S., Achachlouei, B.F., Hesari, J., Emaratpardaz, J. and Tavakolian, R., 2016. Physicochemical properties and nutritional composition of black truffles grown in Iran. Chemistry of Natural Compounds, 52(2): 290-293.
]5] Murcia, M.A., Martínez‐Tomé, M., Vera, A., Morte, A., Gutierrez, A., Honrubia, M. and Jiménez, A.M., 2003. Effect of industrial processing on desert truffles Terfezia claveryi Chatin and Picoa juniperi Vittadini): proximate composition and fatty acids. Journal of the Science of Food and Agriculture, 83(6): 535-541.
]6] Palacios, I., Guillamón, E., García‐Lafuente, A. and Villares, A., 2014. Effects of Freeze-Drying Treatment on the Aromatic Profile of T uber spp. Truffles. Journal of food processing and preservation, 38(3): 768-773.
]7] Rivera, C.S., Blanco, D., Salvador, M.L. and Venturini, M.E., 2010. Shelf‐life extension of fresh Tuber aestivum and Tuber melanosporum truffles by modified atmosphere packaging with microperforated films. Journal of Food Science, 75(4): E225-E233.
]8] Rivera, C.S., Blanco, D., Marco, P., Oria, R. and Venturini, M.E., 2011. Effects of electron-beam irradiation on the shelf life, microbial populations and sensory characteristics of summer truffles (Tuber aestivum) packaged under modified atmospheres. Food Microbiology, 28(1): 141-148.
]9] Nazzaro, F., Fratianni, F., Picariello, G., Coppola, R., Reale, A. and Di Luccia, A., 2007. Evaluation of gamma rays influence on some biochemical and microbiological aspects in black truffles. Food Chemistry, 103(2): 344-354.
]10] Khaleghi, A., Dadbin, E. and Asghari Marjanlou, A., 2019. The Effect of UV-C Irradiation on Rot Control and Postharvest Quality of Greenhouse Tomato (Solanum lycopersicum cv. Newton). Journal of Soil and Plant Interactions-Isfahan University of Technology, 10(3): 13-22.
]11] Severo, J., de Oliveira, I.R., Tiecher, A., Chaves, F.C. and Rombaldi, C.V., 2015. Postharvest UV-C treatment increases bioactive, ester volatile compounds and a putative allergenic protein in strawberry. LWT-Food Science and Technology, 64(2): 685-692.
]12] Simon, R.R., Borzelleca, J.F., DeLuca, H.F. and Weaver, C.M., 2013. Safety assessment of the post-harvest treatment of button mushrooms (Agaricus bisporus) using ultraviolet light. Food and Chemical Toxicology, 56: 278-289.
]13] Sari, L.K., Setha, S. and Naradisorn, M., 2016. Effect of UV-C irradiation on postharvest quality of ‘Phulae’pineapple. Scientia Horticulturae, 213: 314-320.
]14] Bouatia, M., Touré, H.A., Cheikh, A., Eljaoudi, R., Rahali, Y., Idrissi, O.M.B., Khabar, L. and Draoui, M., 2018. Analysis of nutrient and antinutrient content of the truffle (Tirmania pinoyi) from Morocco. International Food Research Journal, 25(1): 174-178.
]15] Gao, M., Feng, L. and Jiang, T., 2014. Browning inhibition and quality preservation of button mushroom (Agaricus bisporus) by essential oils fumigation treatment. Food Chemistry, 149:107-113.
]16] Lister, C.E., Lancaster, J.E., Sutton, K.H. and Walker, J.R., 1994. Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar. Journal of the Science of Food and Agriculture, 64(2):155-161.
]17] Kashyap, G. and Gautam, M.D., 2012. Analysis of vitamin c in commercial and naturals substances by iodometric titration found in nimar and malwa regeion. Journal of Scientific Research in Pharmacy, 1(2): 77-78.
]18] STANDARDS, I. O. & IRAN, I. R. O. 2010. ISIRI 12968. Food and Feed–Maximum Limit of Heavy Metals. ISIRI press Tehran, Iran.
]19] Veeraraghavan, V.P., Hussain, S., Papayya Balakrishna, J., Dhawale, L., Kullappan, M., Mallavarapu Ambrose, J. and Krishna Mohan, S., 2021. A comprehensive and critical review on ethnopharmacological importance of desert truffles: Terfezia claveryi, Terfezia boudieri, and Tirmania nivea. Food Reviews International,: 1-20.
]20] Ammarellou, A., 2007. Protein profile analysis of desert truffle (Terfezia claveryi Chatin). Journal of Food Agriculture and Environment, 5(2): 62-66.
]21] Abdipour, M., Malekhossini, P.S., Hosseinifarahi, M. and Radi, M., 2020. Integration of UV irradiation and chitosan coating: A powerful treatment for maintaining the postharvest quality of sweet cherry fruit. Scientia Horticulturae, 264: 109197.
]22] Manzocco, L., Da Pieve, S. and Maifreni, M., 2011. Impact of UV-C light on safety and quality of fresh-cut melon. Innovative Food Science & Emerging Technologies, 12(1), pp: 13-17.
]23] Cantos, E., García-Viguera, C., de Pascual-Teresa, S. and Tomás-Barberán, F.A., 2000. Effect of postharvest ultraviolet irradiation on resveratrol and other phenolics of cv. Napoleon table grapes. Journal of Agricultural and Food Chemistry, 48(10): 4606-4612.
]24] Pirrello, J., Regad, F., Latche, A., Pech, J.C. and Bouzayen, M., 2009. Regulation of tomato fruit ripening. CAB Reviews, 4(51): 1-14.
]25] González‐Aguilar, G.A., Wang, C.Y., Buta, J.G. and Krizek, D.T., 2001. Use of UV‐C irradiation to prevent decay and maintain postharvest quality of ripe ‘Tommy Atkins’ mangoes. International Journal of Food Science & Technology, 36(7): 767-773.
]26] Basiri, S. and Gheybi, F., 2019. The effect of osmotic dehydration and various methods of drying pumpkin on quality and color of final product. Journal of Food Research, 29: 141- 153
[27] Kıvrak, İ., 2015. Analytical methods applied to assess chemical composition, nutritional value and in vitro bioactivities of Terfezia olbiensis and Terfezia claveryi from Turkey. Food Analytical Methods, 8(5): 1279-1293.
]28] Vahdani, M., Rastegar, S., Rahimizadeh, M., Ahmadi, M. and Karmostaji, A., 2017. Physicochemical characteristics, phenolic profile, mineral and carbohydrate contents of two truffle species, J. Agr. Sci. Tech. 19: 1091-1101.
]29] González‐Aguilar, G.A., Villegas‐Ochoa, M.A., Martínez‐Téllez, M.A., Gardea, A.A. and Ayala‐Zavala, J.F., 2007. Improving antioxidant capacity of fresh‐cut mangoes treated with UV‐C. Journal of Food Science, 72(3): S197-S202.
]30] Liu, C.H., CAI, L.Y., LU, X.Y., HAN, X.X. and YING, T.J., 2012. Effect of postharvest UV-C irradiation on phenolic compound content and antioxidant activity of tomato fruit during storage. Journal of integrative agriculture, 11(1): 159-165.
]31] Portu, J., López, R., Baroja, E., Santamaría, P. and Garde-Cerdán, T., 2016. Improvement of grape and wine phenolic content by foliar application to grapevine of three different elicitors: Methyl jasmonate, chitosan, and yeast extract. Food Chemistry, 201: 213-221.
]32] Vaziri, A., 2020. A pplications of UV-B radiation to increase phenolic compounds of Alo vera L. plants, Journal of Plant Process and Function, 9: 411-421
]33] Amiri, A., Mortazavi, S.M.H., Ramezanian, A., Mahmoodi Sourestani, M., Mottaghipisheh, J., Iriti, M. and Vitalini, S., 2021. Prevention of decay and maintenance of bioactive compounds in strawberry by application of UV-C and essential oils. Journal of Food Measurement and Characterization, 15(6): 5310-5317.
]34] Yalim, S. and Özdemır, Y., 2003. Effects of preparation procedures on ascorbic acid retention in pickled hot peppers. International journal of food sciences and nutrition, 54(4), pp: 291-296.
]35] Mukhopadhyay, S., Ukuku, D.O., Juneja, V. and Fan, X.J.F.C., 2014. Effects of UV-C treatment on inactivation of Salmonella enterica and Escherichia coli O157: H7 on grape tomato surface and stem scars, microbial loads, and quality. Food Control, 44: 110-117
]36] Yagnik, D., Serafin, V. and J Shah, A., 2018. Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans; downregulating cytokine and microbial protein expression. Scientific reports, 8(1): 1-12.