مدل‌سازی و بهینه‌سازی فرمولاسیون سوسیس ماهی حسون (Saurida tumbile) تولید‌شده با حرارت اهمیک به روش سطح پاسخ(RSM)

نویسندگان
1 دانشجو دکتری علوم و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 استاد گروه علوم و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 استادیار گروه علوم و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
4 استادیار گروه مهندسی مکانیک بیوسیستم، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
چکیده
امروزه با تبدیل ماهی­های ارزان و کم مصرف به فرآورده­های دارای ارزش افزوده همانند سوسیس ماهی و همچنین کاربرد روش نوین حرارت­دهی اهمیک به منظور پخت یکنواخت­تر، سریع­تر نسبت به سایر روش­های متداول شدید حرارتی و فرآوری حداقلی می­تواند نقش به­سزایی در تولید محصول ایمن و مغذی ایفا نماید. از این رو هدف از تحقیق حاضر، بررسی برخی از ویژگی­های میکروبی و شیمیایی سوسیس ماهی حسون (Saurida tumbile) تحت تاثیر حرارت­دهی به روش اهمیک و بهینه­سازی فرمولاسیون از طریق بکارگیری روش آماری سطح پاسخ-طرح مرکب مرکزی با در نظرگیری مقادیر مختلف نمک کلرید پتاسیم (3/2-7/0­ درصد)، روغن سویا (14-4 درصد) و ایزوله پروتئین سویا (8/1 - 2/0درصد) می­باشد. بهینه­سازی فرمولاسیون 20 نمونه سوسیس ماهی حرارت­دیده به روش اهمیک با استفاده از نرم افزار Design Expert نسخه 11 صورت گرفت و ارتباط بین متغیرهای مستقل و پنج پاسخ مورد نظر بررسی گردید. نمک کلرید پتاسیم اثر بازدارندگی بر کاهش شمارش کلی میکروبی نمونه­ها نشان داد. اثر متقابل درصد روغن سویا و ایزوله پروتئین سویا تاثیر معنی­داری بر روند تغییرات pH داشتند. افزایش درصد روغن سویا منجر به افزایش اندک pH و سپس کاهش مقدار آن گردید و افزایش ایزوله پروتئین سویا، منجر به کاهش معنی­دار مقدار pH شد. همچنین افزایش درصد نمک و ایزوله پروتئین سویا سبب کاهش افت پخت گردید. افزایش درصد روغن سویا، منجر به کاهش معنی­دار و افزایش ایزوله پروتئین سویا منجر به افزایش ظرفیت نگهداری محصول شد. براساس نتایج بهینه­سازی فرمولاسیون، در سوسیس ماهی با فرمولاسیون محتوی 2 درصد نمک کلرید پتاسیم،6 درصد روغن سویا و 5/1 درصد ایزوله پروتئین سویا بیشترین ظرفیت نگهداری آب، کمترین بار میکروبی و پایین­ترین میزان افت پخت و عدد پراکسید با ارزش pH بهینه مشاهده شد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Modeling and Formulation Optimization of (Saurida tumbil) Greater Lizardfish Sausage Produced by Ohmic heating via response surface methodology (RSM)

نویسندگان English

Sara Shabani 1
Seyed Ebrahim Hosseini 2
Gholam Hasan Asadi 3
Babak Beheshti 4
Shima Yousefi 3
1 Ph.D. Student of Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Professor of Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Assistant Professor of Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 Assistant Professor of Department of Biosystems Engineering, Science and Research branch, Islamic Azad University, Tehran, Iran.
چکیده English



Nowadays, by converting the cheap and commercial low-valued fish into value-added products such as fish sausages, as well as the application of novel ohmic heating method to cook more uniform, faster compared to other thermal severe methods and with minimal processing can play an important role in producing a safe and nutritious product. Therefore, the purpose of this study is to investigate some microbial and chemical characteristics of (Saurida tumbil) greater lizardfish sausage heated by emerging ohmic heating method and formulation optimization was performed by response surface methodology -central composite design (RSM-CCD) by considering KCl (0.7- 2.3 %), Soy Oil ( 4-14 %) and Isolated Soy Protein (0.2-1.8%). Optimization of 20 fish sausage formulation produced by ohmic heating was done by response surface methodology using Design Expert software version 11 and the relationship between the independent variables and the five responses was investigated. KCl has been shown an inhibitory effect on samples total plate count level. The interaction effect of soybean oil and soy protein isolate had a significant effect on pH changes. Increasing soybean oil percentage caused a little increase and subsequently reduction of pH and increasing soy protein isolate led to a significant decrease in pH. Furthermore, higher amount of KCl and soy protein isolate reduced the amount of cooking loss. In addition increase of soy bean oil percentage caused reduction and increase of soy protein isolate lead to increase of water holding capacity. Based on the results of formulation optimization in fish sausages with formulations containing 2% potassium chloride, 6% soybean oil and 1.5% soy protein isolate can increase water holding capacity, the lowest microbial load and the lowest peroxide value, cooking loss and optimized pH.

کلیدواژه‌ها English

Greater lizardfish Sausage
Ohmic heating
KCl
Central composite Rotatable design (CCRD)
[1] Kumar, I., Rajak, D., Kumar Jha, A & Sharma, P.D.(2014). Optimization of ohmic heating of fish using Response Surface Methodology. International of Journal of Food Engineering. 10(3), 481-491.
[2] Mahmoudzadeh M., Motallebi A.A., Hosseini H., Haratian P., Ahmadi H., Mohammadi M. & Khaksar R. (2010). Quality assessment of fish burgers from deep flounder (Pseudorhombus elevatus) and brushtooth lizardfish (Saurida undosquamis) during storage at -18ºC. Iranian Journal of Fisheries Sciences. 9(1) 111-126.
[3] Fisheries Science Research Institute. (2015). Introducing the technical knowledge of producing 20 types of new food and medicine products from aquatic animals. National. Agricultural Research, Education and Extension Organization. Registration number 28-94K. https://agrilib.areeo.ac.ir/book_4185.html
[4] Zhao, Z., Wang, Sh., Li, D & Zhou, Y (2020). Effect of xanthan gum on the quality of low sodium salted beef and property of myofibril proteins. Food Science and Human Wellness. S2213453020302093–. doi:10.1016/j.fshw.2020.09.003.
[5] Rodrigues, I., Gonçalves, L.A., Carvalho, F.AL., Pires, M., JP Rocha, Y., Barros, J.C; Carvalho, L.T; Trindade, M.A. (2019). Understanding salt reduction in fat-reduced hot dog sausages: Network structure, emulsion stability and consumer acceptance. Food Science and Technology International 108201321987267–. doi:10.1177/1082013219872677.
[6] Luna, R., Hashem, M.A., Ali, M.S., Hossain, M.M., Talukder, M.A.K., Islam, M.S. (2013). Effect of Salt and Storage Temperature on Beef Sausage Quality. Progressive Agriculture, 23(1-2), 15–24. doi:10.3329/pa.v23i1-2.16561.
[7] Pires, M. A; Munekata, P. E. S; Baldin, J. C; Rocha, Y. J. P; Carvalho, L. T; dos Santos, I. R; Barros, J.C; Trindade, M. A. (2017). The effect of sodium reduction on the microstructure, texture and sensory acceptance of Bologna sausage. Food Structure, S2213329116300466–. doi:10.1016/j.foostr.2017.05.002.
[8] Kim, T.-K., Yong, H.-I., Jung, S., Kim, H.-W., & Choi, Y.-S. (2021). Technologies for the Production of Meat Products with a Low Sodium Chloride Content and Improved Quality Characteristics—A Review. Foods, 10(5), 957. doi:10.3390/foods10050957.
[9] Gelabert,J., Gou, P., Guerrero, L & Arnau, J.(2003). Effect of sodium chloride replacement on some characteristics of fermented sausages. Meat Science 65,833–839.
[10] World Health Organization. 2012. Effect of increased potassium intake on blood pressure, renal function, blood lipids and other potential adverse effects. Geneva, Switzerland: World Health Organization.
[11] Cepanec, K., Vugrinec, S., Cvetković, T & Ranilović, J. (2017). Potassium Chloride-Based Salt Substitutes: A Critical Review with a Focus on the Patent Literature. Comprehensive Reviews in Food Science and Food Safety, doi:10.1111/1541-4337.12291.
[12] Horita, C. N., Messias, V. C., Morgano, M. A., Hayakawa, F. M., & Pollonio, M. A. R. (2014). Textural, microstructural and sensory properties of reduced sodium frankfurter sausages containing mechanically deboned poultry meat and blends of chloride salts. Food Research International, 66, 29–35.
[13] Corral, S., Salvador,A & Flores, M.(2013). Salt reduction in slow fermented sausages affects the generation of aroma active compounds. Meat Science, 93 (3), 776-785.
[14] Malek, F, (2005). Frying fats and oils and frying technology. Knowledge Boundary Publications - pp 72.
[15] Basiri, L., Sadeghi Mahoonak, A. R., Alami, M & Ghorbani, M. (2012). Effect of heat on functional properties of soybean flour. Journal of Food Science and Technology (Iran). 36(9).
[16] Park, S-Y., Oh, T-S., Kim, G-W & Kim, H-Y. (2020). Quality properties of various dietary fibers as isolated soy protein (ISP) replacements in pork emulsion systems. Journal of Animal Science Technology. 62(1): 94–102. doi: 10.5187/jast.2020.62.1.94.
[17] Naseri, A. R., Taslimi, A., Seyedin, S. M., Haratiyan, P., Abadi, A. R. (2009). Study of the effect of soy protein isolate on macaroni characteristics. Journal of Food Science and Technology (Iran). 6 (21) :1-11.
[18] Kasapis, S. (2009). Developing minced fish products of improved eating quality: an interplay of instrumental and sensory texture. International Journal of Food Properties, 12(1), 11-26.
[19] Bozkurt, H. & Icier, F. (2010). Ohmic Cooking of Ground Beef: Effects on Quality. Journal of Food Engineering, 96, 481-490.
[20] Patyukov, S & Pacinovski, N. (2015). Effect of traditional and ohmic heating on fat stability of pufa-fortified cooked sausages. Macedonian Journal of Animal Science, 5 (2) , 107-112.
[21] Torkian Boldaji, M., Mohammd Borghei, A.M, Beheshti, B, Hosseini, S.E (2014) The process of producing tomato paste by ohmic heating method. Journal of Food Science Technology. doi:10.1007/s13197-014-1424-5.
[22] Shiby, K., Varghese, K., Pandey, M.C., Radhakrishna, K., Bawa technology, A.S.(2012). Application and modeling of ohmic heating: a review. Journal of Food Science and Technology.
[23] Wang, R ., Farid, M.M. (2015). Corrosion and Health Aspects in Ohmic Cooking of Beef Meat Patties. Journal of Food Engineering, 146, 17-22.
[24] Surasani, V. K. R., Raju, C. V., Shafiq, U., Chandra, M. V., & Lakshmisha, I. P. (2019). Influence of protein isolates from Pangas processing waste on physico-chemical, textural, rheological and sensory quality characteristics of fish sausages. LWT, 108662. doi:10.1016/j.lwt.2019.108662.
[25] Gavahian, M., Tiwari, B.K., Chu, Y.H., Ting, Y. & Farahanaky, A. (2019). Food texture as affected by ohmic heating: Mechanisms involved, recent findings, benefits, and limitations. Trends in Food Science & Technology. 86, 328-339.
[26] Rendon A SV, Ambrosio FA, Carrion HM, Llorca E, Hernando I, Quiles A and Diaz SI (2020) Pork meat prepared by different cooking methods. A microstructural, sensorial and physicochemical approach. Meat Science 163: 108089.
[27] Moslemi, M., Hosseini, H., Khaksar, R., Taslimi, A., Khafshdouzan, KH., Shahraz, F. (2010). Effect of cooking and length of storage on the fatty acid composition and microbial, chemical, and sensory properties of 40%-beef products prepared with soybean. Journal of nutrition sciences and food technology, 5(16), 29-38.
[28] Inmanee, P., Kamonpatana, P. & Piral, T. (2019). Ohmic heating effects on Listeria monocytogenes inactivation, and chemical, physical, and sensory characteristic alterations for vacuum packaged sausage during post pasteurization. LWT. Food Science & Technology. 108, 183-189.
[29] Pietrasik, Z & Li-Chan, E.C.Y.(2002). Response surface methodology study on the effects of salt, microbial transglutaminase and heating temperature on pork batter gel properties. Food Research International. 387-396.
[30] Tian X, Wu W, Yu Q, Hou M, Jia F, Li X and Dai R (2016) Quality and proteome changes of beef M. longissimus dorsi cooked using a water bath and ohmic heating process. Innovative Food Science & Emerging Technologies 34: 259-266.
[31] Marchetti, L., Andrés , S.C., Califano, A.N. (2014). Low-fat meat sausages with fish oil: optimization of milk proteins and carrageenan contents using response surface methodology. Meat Science. pp. 1297-1303.
[32] Kim, G.-D., Hur, S. J., Park, T. S., & Jin, S.-K. (2018). Quality characteristics of fat-reduced emulsion-type pork sausage by partial substitution of sodium chloride with calcium chloride, potassium chloride and magnesium chloride. LWT, 89, 140–147. DOI:10.1016/j.lwt.2017.10.033.
[33] Shahab-Lavasani,A. R., Ebrahimzadeh-Mousavi,M. A., Ehsani, M. R.(2008). The Effects of Partial Substitution of NaCl by KCl on Bacterial Total Counts, Mould, Yeast, Coliform, Acid Degree Value and Compositional Properties in UF White Cheese. Quarterly Iranian Journal of food science and technology ,5 (2).
[34] Akesowan, A.(2008). Effect of soy protein isolate on quality of light pork sausages containing konjac flour. African Journal of Biotechnology. 7 (24), 4586-4590.
[35] Arab, S. Z ., Shabanpouri, B ., Pourashuri, P , Rahmani Farah, K. (2017). Effects of different levels of fat and partial substitution of NaCl with KCl on quality and shelf life of silver carp (Hypophthalmichthys molitrix) sausage. Journal of fishery science and technology,6(3).
[36] Eghbalian Rad, M. Sari, A. A., Daraei Garmakhany, A.(2018). Thermal stability optimization of the Soybean oil affected by Thymus daenensis Celak essential oil by use of response surface methodology. Journal of Food Hygiene, 8(32),77-93.
[37] Nazari, F., & Goli, M. (2017). The effect of replacing oil with water and NaCl with KCl on soybean oil hydrolysis and oxidation in canned skipjack tuna fish at the end of the 18-month shelf life. Food Science and Biotechnology, 26(1), 49–53. doi:10.1007/s10068-017-0007-4
[38] Colmenero, F. J., Ayo, M. J & Carballo, J. (2005). Physicochemical properties of low sodium frankfurter with added walnut: effect of transglutaminase combined with caseinate, KCl and dietary fibre as salt replacers. Meat Science, 69(4), 781–788. doi:10.1016/j.meatsci.2004.11.011.
[39] De Sousa, A. M. B., de Araujo Alves, R., Madeira, D. S. S., Santos, R. M., Pereira, A. L. F., de Oliveira Lemos, T., & Abreu, V. K. G. (2020). Storage of beef burgers containing fructooligosaccharides as fat replacer and potassium chloride as replacing sodium chloride. Journal of Food Science and Technology. doi:10.1007/s13197-020-04354-0 .
[40] Guan, H., Feng, Ch., Xu, X., Sun, W., Han, J ., Liu, D & Diao , X. (2022). Effect of high-pressure processing enzymatic hydrolysates of soy protein isolate on the quality characteristics and oxidation stability of emulsion sausage. British Food Journal. https://doi.org/10.1108/bfj-04-2021-0357.
[41] Biswas, S., Chakraborty, A., Sarkar S., Barpuzari R. N. & Barpuzari T. (2007). Effect of incorporation of chicken fat and skin on the quality of chicken sausage. The Journal of Poultry Science, 44: 111-115.
[42] Chin, KB., Keeton, JT., Longnecker, MT & Lamkey, JW. (2000). Utilization of soy protein isolate and konjac blends in low-fat bologna. Meat Science, 53: 87-96.
[43] Rahman, A., Hosseini, S.E., Otadi, M.(2013). Effect of other salts (CaCl2, MgCl2 and KCl), Fat and Gellan Gum on Texture of sausage. Innovation in food Science and technology ,5 , 1 (15),1 -11.
[44] Mahdavian Mehr, H., Koocheki, A., Mohebbi, M. (2015). Effect of soy protein isolates concentration and batter temperature on flow properties of batter and quality of deep-fried chicken nugget. Iranian Food Science and Technology. 11, 5, 608-619.