کاربرد ترکیبات فیتوشیمیایی استخراج شده از عصاره پوست لیموشیرین از طریق جذب و واجذب توسط گرافن اکساید به‌عنوان آنتی‌اکسیدان در روغن سرخ‌کردنی

نویسندگان
واله شریف نصیریان 1
سید احمد شهیدی 1
حسن طاهر منصوری 2
فرشته چکین 2
1 گروه علوم و صنایع غذایی، دانشگاه آزاد اسلامی واحد آیت ا… آملی، آمل، ایران
2 گروه شیمی، دانشگاه آزاد اسلامی واحد آیت ا… آملی، آمل، ایران
10.52547/fsct.19.122.236
چکیده
در این پژوهش، فلاونوئیدهای پوست لیموشیرین توسط جاذب گرافن اکساید از طریق جذب و سپس واجذب استخراج شدند و ویژگی‌های عصاره واجذبی شامل تعیین محتوای فنلی کل، محتوای کل فلاونوئیدها و قابلیت روبندگی رادیکال‌های آزاد DPPH ارزیابی شدند. سپس اثر فعالیت آنتی‌اکسیدانی عصاره واجذبی در روغن فرموله شده که شامل روغن حاوی عصاره‌ی واجذبی از نانو جاذب‌ها در غلظت‌های 250، 500 و ppm1000 بود مورد بررسی قرار گرفت و با روغن حاوی آنتی‌اکسیدان سنتزی BHT در غلظت ppm‌200 و نمونه شاهد مقایسه گردید. نتایج نشان داد میزان فنل کل برای عصاره اصلی پوست لیموشیرین و عصاره واجذبی به ترتیب 20/19767 و 56/5861 میکروگرم بر عصاره بود و میزان فلاونوئید کل برای عصاره اصلی پوست لیموشیرین و عصاره واجذبی به ترتیب 552/33 و 446/3 میکروگرم بر عصاره به دست آمد. اﻧﺪﻳﺲ ﭘﺮاﻛﺴﻴﺪ، عدد تیوباربیتوریک اسید، دی ان مزدوج و شاخص رنگی روغن با افرایش زمان حرارت دهی افزایش‌یافته و بیشترین میزان افزایش در تیمار شاهد فاقد آنتی‌اکسیدان مشاهده گردید و تیمارهای حاوی عصاره واجذبی (فلاونوئید جذب‌شده توسط گرافن اکساید از عصاره پوست لیمو شیرین)500 و 1000 ppm، قابل‌رقابت با آنتی‌اکسیدان BHT بودند. درنهایت می‌توان بیان داشت عصاره واجذبی پوست لیموشیرین به‌عنوان منبعی غنی از آنتی‌اکسیدان‌های طبیعی قابلیت جایگزینی آنتی‌اکسیدان‌های سنتزی در فرمولاسیون روغن‌های خوراکی را دارد.
کلیدواژه‌ها
آنتی‌اکسیدان
پوست لیموشیرین
عصاره واجذبی
فلاونوئیدها
نانوجاذب

موضوعات

عنوان مقاله English

The adsorption and desorption of phytochemicalfrom lemon peel extract by the graphene oxide and its application as antioxidant in frying oil

نویسندگان English

Valeh Sharif Nasirian 1
Seyed-Ahmad Shahidi 1
Hasan Tahermansouri 2
Fereshteh Chekin 2
1 Department of Food Science and Technology, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran
2 Department of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
چکیده English

In this study, the graphene oxide was used as adsorbent for the separation (adsorption and desorption) of flavonoids from lemon peel. Properties of desorption extract (total phenolic content, total flavonoid content, scavenging ability of DPPH free radicals) were determined. Antioxidant activity of desorption extract at three levels of 250, 500 and 1000 ppm and BHT synthetic antioxidant at 200 ppm in antioxidant-free frying oil were evaluated by measuring peroxide value, thiobarbituric acid test, conjugated DN and color index. Each gram of lemon peel extract contained 19767.20 μg phenolic compounds and 33.552 μg flavonoids on dry matter. Each gram of desorption extract contained 5861.56 μg phenolic compounds and 3.446 μg flavonoids on dry matter. Peroxide value, thiobarbituric acid, conjugated DN and color index of oil increased with increasing heating time and the highest increase was observed in the control treatment without antioxidants. Treatments containing desorption extract at 500 and 1000 ppm, can compete with BHT antioxidant. The results of this study indicated that desorption extract can be used as a replacement for synthetic antioxidants in oil.

کلیدواژه‌ها English

antioxidants
Desorption extract
flavonoids
Nanosorbent
Sweet lemon peel
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