بررسی ویژگی‌های شیمیایی و فعالیت ضدمیکروبی عصاره متانولی ترشک بر باکتری‌های انتروباکتر ائروژنز، سالمونلا تیفی، استافیلوکوکوس اورئوس و استرپتوکوکوس پیوژنز: یک مطالعه آزمایشگاهی

نویسندگان
محمد نوشاد
بهروز علیزاده بهبهانی
استادیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.52547/fsct.18.117.109
چکیده
در سال‌های اخیر توجه ویژه‌ای به داروهای با منشأ گیاهی معطوف شده است. گیاهان دارویی دارای ترکیبات متنوعی هستند که قابلیت پوشش طیف وسیعی از اثرات درمانی را دارا می‌باشند. در این پژوهش، عصاره متانولی گیاه ترشک (Rumex alveollatus L.) به روش خیساندن تهیه شد و محتوای فنول کل، فلاونوئید کل، فعالیت آنتی‌اکسیدانی (مهار رادیکال آزاد DPPH و ABTS) و خاصیت ضدمیکروبی (دیسک دیفیوژن، چاهک آگار و حداقل غلظت بازدارندگی و کشندگی) آن در برابر باکتری‌های انتروباکتر ائروژنز، سالمونلا تیفی، استافیلوکوکوس اورئوس و استرپتوکوکوس پیوژنز بررسی گردید. عصاره متانولی ترشک حاوی محتوای فنول کل 90/91 میلی‏گرم گالیک اسید در گرم و فلاونوئید کل 60/48 میلی‌گرم کوئرستین در گرم بود. نتایج فعالیت آنتی‌اکسیدانی نشان داد که عصاره متانولی قادر به مهار رادیکال‌های آزاد DPPH (58/69 درصد) و ABTS (59/80 درصد) می‌باشد. غلظت‌های بالای عصاره از رشد میکروارگانیسم‌های مورد مطالعه جلوگیری نمود و باکتری‌های انتروباکتر ائروژنز و استافیلوکوکوس اورئوس به ترتیب مقاوم‌ترین و حساس‌ترین باکتری­ها در برابر عصاره متانولی ترشک بودند. بنابراین، عصاره متانولی ترشک می‌تواند بعنوان ترکیبی مؤثر در کنترل عفونت‌های باکتریایی و اکسایش ترکیبات لیپیدی معرفی گردد.
کلیدواژه‌ها
گیاه ترشک
عصاره متانولی
فعالیت ضدمیکروبی
اثر آنتی‌اکسیدانی
ترکیب زیست فعال

موضوعات

عنوان مقاله English

Investigation of chemical properties and antimicrobial activity of Rumex alveollatus methanolic extract on Enterobacter aerogenes, Staphylococcus aureus, Salmonella typhi, and Streptococcus pyogenes: An “in vitro” study

نویسندگان English

Mohammad Noshad
Behrooz Alizadeh behbahani
1Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

In recent years, special attention has been devoted to plant-derived drugs. Herbal medicines contain versatile compounds with a vast range of therapeutic effects. In this study, methanolic extract of Rumex alveollatus L. was obtained thorough the maceration method, and its total phenol content, total flavonoid content, antioxidant activity (DPPH and ABTS radical scavenging methods) and antimicrobial property (disk diffusion agar, well diffusion agar, and minimum inhibitory/bactericidal concentration) against Enterobacter aerogenes, Staphylococcus aureus, Salmonella typhi, and Streptococcus pyogenes were evaluated. The methanolic extract of Rumex alveollatus contained 91.90 mg gallic acid/g total phenols and 48.60 mg quercetin/g total flavonoids. Antioxidant activity results showed that the extract was able to scavenge DPPH (69.58%) and ABTS (80.59%) free radicals. The growth of microorganisms was inhibited in the presence of high concentrations of the extract, and E. aerogenes and S. aureus were the most resistant and sensitive bacterial species to the methanolic extract of Rumex alveollatus. Therefore, Rumex alveollatus methanolic extract could be introduced as an effective compound in controlling bacterial infections and oxidation of lipid-based compounds.

کلیدواژه‌ها English

Rumex alveollatus
methanolic extract
Antimicrobial activity
Antioxidant effect
Bioactive compound
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