ارزیابی فعالیت شیمیایی و اثر ضدقارچی اسانس‌ پنج انگشت (Vitex agnus-castus) بر پنی‌سیلیوم دیجیتاتوم و پنی‌سیلیوم ایتالیکوم عامل کپک‌زدگی میوه‌ پرتقال

نویسندگان
مصطفی رحمتی جنیدآباد 1
بهروز علیزاده بهبهانی 2
1 استادیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 استادیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.52547/fsct.18.114.82
چکیده
اسانس‌های گیاهی بطور گسترده‌ای بعنوان نگهدارنده طبیعی جهت کاهش سمیت قارچ‌کش‌های شیمیایی و آلودگی زیست‌محیطی بکار می‌روند. این مطالعه با هدف بررسی اثر ضدقارچی اسانس گیاه پنج انگشت بر کپک‌های پنی‌سیلیوم دیجیتاتوم و پنی‌سیلیوم ایتالیکوم عامل کپک‌زدگی میوه پرتقال انجام گرفت. اسانس گیاه پنج انگشت با کمک روش تقطیر با آب استخراج گردید و راندمان استخراج اسانس 95/0 درصد وزنی/وزنی بود. محتوای فنول و فلاوونوئید کل اسانس نیز بررسی شد و نتایج نشان داد که اسانس غنی از ترکیبات فنولی (mg GAE/g 74/91) و فلاوونوئیدی (mg QE/g 32/52) می‌باشد. بررسی فعالیت ضد اکسایشی اسانس پنج انگشت نشان داد که این ترکیب زیست فعال بطور مؤثری قادر به مهار رادیکال‌های آزاد DPPH (μg/mL 62/48 IC50 =) و ABTS (μg/mL 50/40 IC50 =) می‌باشد. فعالیت ضدقارچی اسانس گیاه پنج انگشت در برابر کپک‌های پنی‌سیلیوم دیجیتاتوم و پنی‌سیلیوم ایتالیکوم مطابق روش‌های دیسک دیفیوژن آگار، انتشار چاهک در آگار و حداقل غلظت مهارکنندگی/کشندگی بررسی شد و پنی‌سیلیوم دیجیتاتوم نسبت به اسانس حساس‌تر بود و غلظت پایین‌تری از این ترکیب زیست فعال قادر به جلوگیری از رشد و یا از بین بردن این کپک بود. بطور کلی، نتایج این مطالعه نشان داد که اسانس گیاه پنج انگشت غنی از ترکیبات زیست فعال با قابلیت ضد اکسایشی و ضدقارچ قابل‌توجهی می‌باشد و می‌توان این ترکیب را بعنوان نگهدارنده طبیعی جهت افزایش عمر انبارمانی محصولات غذایی بکار برد.
کلیدواژه‌ها
پرتقال
کپک‌زدگی
اسانس
گیاه پنج انگشت
فعالیت ضد اکسایشی
اثر ضدقارچی

موضوعات

عنوان مقاله English

Evaluation of chemical activity and antifungal effect of Vitex agnus-castus essential oil on Penicillium digitatum and Penicillium italicum causing orange rot

نویسندگان English

Mostafa Rahmati-Joneidabad 1
Behrooz Alizadeh behbahani 2
1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

Plant essential oils are frequently used as natural preservatives to decrease the toxicity of synthetic fungicides and environmental pollution. This study was aimed to investigate the antifungal effect of Vitex agnus-castus essential oil on Penicillium digitatum and Penicillium italicum causing orange rot. The essential oil of V. agnus-castus was extracted by the hydrodistillation method and the extraction yield was 0.95% w/w. The total phenol and flavonoids content of the essential oil were also measured and the results showed that the essential oil is rich in phenolics (91.74 mg GAE/g) and flavonoids (52.32 mg QE/g). The evaluation of antioxidant activity of the V. agnus-castus essential oil showed that the bioactive compound is able to effectively scavenge DPPH (IC50 = 48.62 μg/mL) and ABTS (IC50 = 40.50 μg/mL) free radicals. The antifungal activity of the V. agnus-castus essential oil was evaluated against P. digitatum and P. italicum, based on disk diffusion agar, well diffusion agar, and minimum inhibitory/fungicidal concentration methods. And P. digitatum was more sensitive to the essential oil and lower concentration of the bioactive compound was able to inhibit the growth of or kill the species. In general, the results of this study showed that the V. agnus-castus essential oil is rich in bioactive compounds with remarkable antioxidant and antifungal activities, and it could be applied as a natural preservative to increase the shelf-life of food products.

کلیدواژه‌ها English

Orange
Rotting
Essential oil
Vitex agnus-castus
Antioxidant activity
antifungal effect
[1] Khoshtaghaza, M. H., & Taghinezhad, E. (2017). Investigation effect of particle Nano coating on storage quality properties of Thomson orange. Food Science and Technology, 61(13), 121-133.
[2] Golshan Tafti, A., & Baradaran, G. (2018). Study of the effect of essential oils in controlling of blue and green molds rot in orange fruit. Journal of Food Microbiology, 6(3), 12-21.
[3] Rahmati-Joneidabad, M., & Alizadeh-Behbahani, B. (2021). Identification of chemical compounds, antioxidant potential, and antifungal activity of Thymus daenensis essential oil against spoilage fungi causing apple rot. Iranian Food Science and Technology Research Journal. 10.22067/IFSTRJ.V18I1.87595.
[4] Heydari, S., Jooyandeh, H., Alizadeh-Behbahani, B., & Noshad, M. (2019). In vitro Determination of Chemical Compounds and Antibacterial Activity of Lavandula Essential oil against some Pathogenic Microorganisms. Scientific Journal of Ilam University of Medical Sciences, 27(4), 77-89.
[5] Alizadeh-Behbahani, B., Noshad, M., & Sahraiyan, B. (2021). Investigation of the minimum inhibitory concentration and the minimum bactericidal concentration of Eucalyptus globulus essential oil on a number of pathogenic bacteria and the cause of food spoilage. Food Science and Technology, 18(110), 49-57.
[6] Alizadeh-Behbahani, B., Noshad, M., & Falah, F. (2019). Investigation of antimicrobial activity of Fennel essential oil on some pathogenic microorganisms causing infection and food poisoning and its interaction with kanamycin antibiotic. Food Science and Technology, 16(91), 233-241.
[7] Heydari, S., Jooyandeh, H., Alizadeh Behbahani, B., & Noshad, M. (2020). The impact of Qodume Shirazi seed mucilage‐based edible coating containing lavender essential oil on the quality enhancement and shelf life improvement of fresh ostrich meat: An experimental and modeling study. Food Science & Nutrition, 8(12), 6497-6512.
[8] Hosseinzadeh Sahafi, H., Talebzadeh, S. A., & Naji, T. (2018). The role of different concentration of Vitex agnus-castus on control of ripeness of gonad of Carassius auratus. Journal of Aquaculture Development, 12(3), 63-74.
[9] Nasri, S., & Ebrahimi Vostakolaei, S. (2007). Therapeutic effects of Vitex agnus-castus L. Journal of Babol University of Medical Sciences, 8(7), 49-53.
[10] Sarikurkcu, C., Arisoy, K., Tepe, B., Cakir, A., Abali, G., & Mete, E. (2009). Studies on the antioxidant activity of essential oil and different solvent extracts of Vitex agnus castus L. fruits from Turkey. Food and Chemical Toxicology, 47(10), 2479-2483.
[11] Asdadi, A., Hamdouch, A., Oukacha, A., Moutaj, R., Gharby, S., Harhar, H., ... & Hassani, L. I. (2015). Study on chemical analysis, antioxidant and in vitro antifungal activities of essential oil from wild Vitex agnus-castus L. seeds growing in area of Argan Tree of Morocco against clinical strains of Candida responsible for nosocomial infections. Journal de mycologie medicale, 25(4), e118-e127.
[12] Alizadeh-Behbahani, B., Falah, F., Vasiee, A., Tabatabaei Yazdi, F., & Mortazavi, S. A. (2019). Antimicrobial effect of Citrus aurantium essential oil on some food-borne pathogens and its determination of chemical compounds, total phenol content, total flavonoids content and antioxidant potential. Food Science and Technology, 16(87), 275-288.
[13] Tanavar, H., Barzegar, H., Alizadeh Behbahani, B., & Mehrnia, M. A. (2020). Mentha pulegium essential oil: chemical composition, total phenolic and its cytotoxicity on cell line HT29. Iranian Journal Food Science and Technology Research, 16(5), 643-653.
[14] Zahid, H., Rizwani, G. H., & Ishaqe, S. (2016). Phytopharmacological review on Vitex agnus-castus: a potential medicinal plant. Chinese Herbal Medicines, 8(1), 24-29.
[15] Ulukanli, Z., Çenet, M., Öztürk, B., Bozok, F., Karabörklü, S., & Demirci, S. C. (2015). Chemical characterization, phytotoxic, antimicrobial and insecticidal activities of Vitex agnus-castus’ essential oil from East Mediterranean Region. Journal of Essential Oil Bearing Plants, 18(6), 1500-1507.
[16] Ricarte, L. P., Bezerra, G. P., Romero, N. R., Da Silva, H. C., Lemos, T. L. G., Arriaga, A. M. C. et al. (2020). Chemical composition and biological activities of the essential oils from Vitex-agnus castus, Ocimum campechianum and Ocimum carnosum. An Acad Bras Cienc, 92(1), e20180569.
[17] Rezaei, M., Razmjoo, J., Ehtemam, M. H., Karimmojeni, H., & Zahedi, M. (2019). The interaction between shade and drought affects essential oil quantity and quality of Vitex agnus-castus L. leaves and seeds. Industrial Crops and Products, 137, 460-467.
[18] Katiraee, F., Mahmoudi, R., Tahapour, K., Hamidian, G., & Emami, S. J. (2015). Biological properties of Vitex agnus-castus essential oil (Phytochemical component, antioxidant and antifungal activity). Biotechnology and Health Sciences, 2(2), e26797.
[19] Gökbulut, A., Özhan, O., Karacaoğlu, M., & Şarer, E. (2010). Radical Scavenging Activity and Vitexin Content of Vitex agnus-castus Leaves and Fruits. FABAD Journal of Pharmaceutical Science, 35, 85–91.
[20] Pinto, P. C., Saraiva, M. L. M., Reis, S., & Lima, J. L. (2005). Automatic sequential determination of the hydrogen peroxide scavenging activity and evaluation of the antioxidant potential by the 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay in wines by sequential injection analysis. Analytica chimica acta, 531(1), 25-32.
[21] Bai, W., Wang, Q., Zeng, X., Fu, J., Liu, Y., & Dong, H. (2017). Antioxidant activities of chicken peptide‐Maillard reaction products (CP‐MRPS) derived from chicken peptides and d‐glucose system. Journal of Food Processing and Preservation, 41(2), e13041.
[22] Noshad, M., Alizadeh-Behbahani, B., & Dehghani, S. (2020). Improving oxidative and microbial stability of beef by using a bioactive edible coating obtained from Barhang-e-Sagheer seed mucilage and loaded with Avishan-e-Baghi. Food Science and Technology, 101(17), 1-13.
[23] Hojjati, M., & Alizadeh-Behbahani, B. (2021). Evaluation of the effect of aqueous and methanolic extraction methods on the antioxidant and antimicrobial characteristics of Allium jesdianum extract: in vitro study. Iranian Food Science and Technology Research Journal, 17(1), 83-91.
[24] Alizadeh Behbahani, B., Falah, F., Lavi Arab, F., Vasiee, M., & Tabatabaee Yazdi, F. (2020). Chemical Composition and Antioxidant, Antimicrobial, and Antiproliferative Activities of Cinnamomum zeylanicum Bark Essential Oil. Evidence-Based Complementary and Alternative Medicine, 2020.
[25] Behbahani, B. A., Noshad, M., & Falah, F. (2019). Cumin essential oil: Phytochemical analysis, antimicrobial activity and investigation of its mechanism of action through scanning electron microscopy. Microbial pathogenesis, 136, 103716.
[26] Stojković, D., Soković, M., Glamočlija, J., Džamić, A., Ćirić, A., Ristić, M., & Grubišić, D. (2011). Chemical composition and antimicrobial activity of Vitex agnus-castus L. fruits and leaves essential oils. Food Chemistry, 128(4), 1017-1022.
[27] Pina‐Vaz, C., Gonçalves Rodrigues, A., Pinto, E., Costa‐de‐Oliveira, S., Tavares, C., Salgueiro, L., ... & Martinez‐de‐Oliveira, J. (2004). Antifungal activity of Thymus oils and their major compounds. Journal of the European Academy of Dermatology and Venereology, 18(1), 73-78.