کاربرد روش جدید استخراج جفت‌شده به کروماتوگرافی مایع با کارایی بالا برای پایش آنتی‌بیوتیک‌های تتراسایکلینی در شیر گاو

نویسندگان
حافظ راثی 1
محمدرضا افشارمقدم 2
جلیل خندقی 3
1 دانش‌آموخته کارشناسی‌ارشد علوم و صنایع غذایی، واحد سراب، دانشگاه آزاد اسلامی ، سراب، ایران
2 استادیار مرکز تحقیقات آنالیز دارویی، دانشگاه علوم پزشکی تبریز، تبریز، ایرانواستادیار مرکز ایمنی غذا و دارو، دانشگاه علوم پزشکی تبریز، تبریز، ایران
3 استادیار گروه علوم و صنایع غذایی، واحد سراب، دانشگاه آزاد اسلامی ، سراب، ایران
10.52547/fsct.18.113.339
چکیده
استفاده بی رویه از آنتی‌بیوتیک‌ها در صنعت پرورش دام سبب تجمع باقیمانده‌های آنتی‌بیوتیکی در محصولات دامی مختلف مانند گوشت و شیر خواهد‌شد. مصرف مواد غذایی حاوی آنتی‌بیوتیک‌ها مشکلاتی مانند افزایش مقاومت‌های آنتی‌بیوتیکی را به‌دنبال دارد، ضمن اینکه تجمع آن ها در بدن می‌تواند منجر به جهش‌زایی و ایجاد انواع سرطان‌ها، آسیب‌های کبدی و سقط جنین شود. به همین دلیل پایش باقیمانده آنتی‌بیوتیک‌ها بخصوص در محصولات غذایی پرمصرف و سودمند مانند شیر و لبنیات از اهمیت ویژه‌ای برخوردار است. در این کار پژوهشی روش میکرواستخراج فاز جامد پخشی برای استخراج آنتی‌بیوتیک‌های تتراسایکلین، اکسی تتراسایکلین، کلرتتراسایکلین و داکسی‌سایکلین از 50 نمونه‌ شیر و اندازه‌گیری کمی آنها به‌روش کروماتوگرافی مایع با کارایی بالا توسعه داده‌شد. برای این منظور اثر پارامترهای مختلف موثر در کارایی استخراج مانند حجم عامل رسوب دهنده، نوع و مقدار جاذب، قدرت یونی، زمان و دبی دمیدن هوا، نوع و حجم سورفاکتانت و نوع و حجم حلال‌شوینده بهینه‌سازی شد. نتایج تحقیق نشان داد که همه نمونه‌های شیر دارای باقیمانده تتراسایکلین بوده و مقدار آن در محدوده 146 تا 319 نانوگرم در میلی‌لیتر متغیراست. دیگر آنتی‌بیوتیک‌های مورد مطالعه در نمونه‌ها یافت نشد. از مزایای روش استخراج پیشنهادی می‌توان به قدرت جداسازی بالا و امکان آنالیز مخلوط آنالیت‌ها با حساسیت بالا اشاره کرد به‌طوریکه تحت شرایط بهینه، کارایی روش پیشنهادی 91-80 درصد و حدود تشخیص در محدوده‌ی 0.33-0.17 نانوگرم در میلی‌لیتر و محدوده‌ی خطی روش در گستره‌ی 0.63-2000 نانوگرم در میلی‌لیتر بدست آمدند.
کلیدواژه‌ها
تتراسایکلین
شیر
میکرواستخراج فاز جامد پخشی
کروماتوگرافی مایع با کارایی بالا

موضوعات

عنوان مقاله English

Application of a new extraction method coupled to high performance liquid chromatography for tetracyclines monitoring in cow milk

نویسندگان English

Hafez Rasi 1
MohammadReza AfsharMogaddam 2
Jalil Khandaghi 3
1 M.Sc Graduate of Food Science and Technology, Sarab Branch, Islamic Azad University, Sarab, Iran
2 Assistant Professor of Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, IranandAssistant Professor of Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Assistant Professor of Department of Food Science and Technology, Sarab Branch, Islamic Azad University, Sarab, Iran
چکیده English

Excessive use of antibiotics in the production of livestock has led to the accumulation of antibiotic residues in various livestock products such as meat and milk. Consumption of foods containing antibiotics leads to problems such as increased antibiotic resistance, while their accumulation in the body can lead to mutagenesis and cancers, liver damage and abortion. For this reason, monitoring residual antibiotics, especially in high-consumption and beneficial food products such as milk and dairy products, is of particular importance. In this research, a dispersive solid-phase micro-extraction method for the extraction of tetracycline, oxytetracycline, chlortetracycline and doxycycline antibiotics from 50 milk samples and their quantitative measurement by HPLC was developed. For this purpose, the effect of various parameters affecting the extraction efficiency such as the volume of the precipitating agent, the type and amount of solid adsorbent, ionic strength, aeration time and flow rate, type and amount of surfactant and type and volume of elution solvent were optimized. The results showed that all milk samples contained tetracycline residue and its concentration varied in the range of 146 to 319 ng / ml. No other antibiotics were found in the samples. The advantages of the proposed extraction method include high separation power and the possibility of analyzing a mixture of highly sensitive analytes, so that under optimal conditions, recoveries of the proposed method ranging from 80 to 91%, the LOD were in the range of 0.17-0.33 ng / ml and the linear range was in the range of 0.63-2000 ng / ml.

کلیدواژه‌ها English

Tetracycline
Milk
dispersive solid-phase micro-extraction
HPLC
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