بررسی تأثیر روش تولید فیلم (کامپوزیت یا دولایه) و نسبت بیوپلیمرها بر خصوصیات فیزیکوشیمیایی فیلم‌ فعال کیتوزان - ایزوله پروتئین آب پنیر به روش سطح پاسخ

نویسندگان
خورشید حسین زاده
اصغر خسروشاهی اصل
محمد علیزاده خالدآباد
هادی الماسی
گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه ارومیه
10.52547/fsct.18.113.59
چکیده
هدف از این تحقیق بررسی امکان تهیه فیلم­های کامپوزیت و دولایه کیتوزان و ایزوله پروتئین آب پنیر حاوی اسانس زیره و نانوفیبر کیتوزان بود و بررسی خواص آن بود. امکان تولید فیلم کامپوزیت با کاهش pH ایزوله پروتئین به پایین­تر از pH ایزوالکتریک فراهم شد. فیلم دولایه نیز با ریختن محلول WPI روی فیلم خشک شده‌ی کیتوزان حاصل شد. مقادیر ترکیب فعال و نانوپرکننده ثابت بود (mg/100ml 125) و تأثیر نسبت کیتوزان به WPI و نوع فیلم (لامینه یا کامپوزیت) با استفاده از روش سطح پاسخ بر روی خواص فیلم بررسی شد. پارامترهای مربوط به رنگ، زاویه تماس آب، جذب رطوبت، حلالیت، نفوذپذیری به بخار آب و خواص مکانیکی فیلم­ها بررسی شد. با استفاده از تابع مطلوبیت و بر اساس نتایج بدست آمده، فرمولاسیون بهینه فیلم­های کامپوزیت (14/65 درصد WPI و 85/34 درصد کیتوزان) و دو لایه (01/49 درصد WPI و 99/50 درصد کیتوزان) بدست آمد. آنالیز ریزساختار فیلم­های بهینه با استفاده از FTIR، FE-SEM و XRD انجام شد. نتایج FE-SEM نشان داد در فیلم کامپوزیت با وجود ساختار ناهمگن، ناسازگاری بین دو پلیمر ایجاد نشد و همچنین در فیلم دو لایه اتصال خوبی بین دو لایه بوجود آمد. نتایج XRD نشان داد فیلم کامپوزیت ساختار نیمه کریستالی دارد و اختلاف معنی­داری بین شدت پیک‌ها و خواص کریستالی فیلم کامپوزیت و دو لایه وجود ندارد. در نتایج FTIR اختلاف کمی بین طیف دو نوع فیلم مشاهده شد. نتایج بررسی فیلم­های بهینه نشان داد، خصوصیات فیلم کامپوزیت در مقایسه با فیلم دو لایه مطابقت بیشتری با مدل پیش بینی شده دارد. اما فیلم لامینه از خصوصیات مورفولوژیکی بهتر و پتانسیل کاربرد بیشتر در بسته بندی یا مواد غذایی برخوردار بود و برای نگهداری محصولات غذایی مختلف قابل پیشنهاد است.
کلیدواژه‌ها
فیلم زیست تخریب پذیر
کامپوزیت
لامیناسیون
ریزساختار
خواص مکانیکی
بهینه سازی

موضوعات

عنوان مقاله English

Investigating the effect of film preparation method (composite or laminated) and biopolymers’ proportion on the physicochemical properties of active chitosan-whey protein isolate film by response surface method (RSM)

نویسندگان English

Khorshid Hosseinzadeh
Asghar Khosrowshahi Asl
Mohammad Alizadeh Khaledabad
Hadi Almasi
Department of Food Science, Faculty of agriculture, Urmia university
چکیده English

The aim of this research was to investigate the feasibility of composite and laminated chitosan (CH) and whey protein isolate (WPI) film preparation which was containing cumin essential oil and chitosan nanofiber. Production of composite film was possible by decreasing WPI pH to below its isoelectric pH. Double-layer was also prepared by pouring WPI solution on dried CH film. amount of active agent and nanofiller was fixed (125 mg/100ml) and only the effect of CH/WPI ration and film type (laminated or composite) on the properties of films was arudied by RSM analytical method. The color, watercontact angle, moisture absorption, solubility, water vapor permeability and mechanical properties of films were determined. By using desirability function and according to the results, optimum formulation of composite film (65.14% WPI and 34.85% CH) and laminated film (49.01% WPI and 50.99% CH) was achieved. Microstructure of optimized films was analyzed by FTIR, FE-SEM and XRD tests. Results of FE-SEM indicated a heterogenous stracture in composite film but without any phase separation. Also, at the laminatred film good attachment between two layers of CH and WPI was observed. Semi-crystalline stracture of composite film was approved by XRD analysis and there was no detectable difference between peak intensity and crystalline stracture of composite and laminated film. there was a slight difference in the FTIR spectra of composite and laminated films. According to the results, the characteristics of composite film in comparison to laminated film had better accordance with the used prediction model. But the laminated film exhibited better morphological characteristics and could be suggested for preservation of different foods due to its high potential for use in food packaging.

کلیدواژه‌ها English

Biodegradable film
Composite
Lamination
Microstracture
Mechanical properties
Optimization
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