تاثیر نوع حلال بر قدرت آنتی اکسیدانی، میزان فنل و فلاونوئید گیاه Postia puberula

نویسندگان
گروه زیست شناسی، دانشکده علوم پایه، دانشگاه لرستان، خرم آباد
چکیده
هدف از این مطالعه بررسی تاثیر نوع حلال بر قدرت آنتی‌اکسیدانی و میزان فنل و فلاونوِئید گیاه Postia puberula متعلق به خانواده آستراسه است. جهت بررسی اثرات آنتی اکسیدانی میزان، فنل و فلاونوئید عصاره­های اتانولی، متانولی، آبی و هیدروالکلی تهیه شد. بررسی اثرات آنتی اکسیدانی نیز با دو روش 2و2 دی فنیل 1-پیکریل هیدرازیل (DPPH) و بتاکاروتن- لینولئیک اسید صورت گرفت. جهت سنجش محتوای ترکیبات فنلی و فلاونوئیدی به ترتیب از شاهدهای گالیک اسید و کوئرسیتین استفاده شد. نتایج نشان داد که نوع حلال مورد استفاده در عصاره­گیری می­تواند بر اثرات آنتی اکسیدانی، میزان فنل و فلاونوئید موثر باشد ، به طوری که بیشترین میزان فعالیت آنتی اکسیدانی در هر دو روش مربوط به عصاره هیدروالکلی است، همچنین بیشترین میزان فنل در عصاره هیدروالکلی و بیشترین میزان فلاونوئید در عصاره اتانولی مشاهده شد.
کلیدواژه‌ها

عنوان مقاله English

The effect of extraction solvent on antioxidant activity, total phenol content and total flavonoid content of Postia puberula

نویسندگان English

Parvaneh Hemmati Hassan Gavyar
Hamzeh Amiri
گروه زیست شناسی، دانشکده علوم پایه، دانشگاه لرستان، خرم آباد
چکیده English

The propose of this study is evaluation effects of different solvents on antioxidant activity and phenol and flavonoid content of Postia puberula (Asteracea). The obtained ethanol, methanol, water and hydroalchol extract were investigated for antioxidant activity and phenol and flavonoid content. The antioxidant activity were measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid assay, also phenol and flavonoid content were measured by gallic acid and quercetin as standard compound. The result of this study showed that the the different solvents can effective on antioxidant activity, phenol and flavonoid content so that, in both method hydroalchol extract has more antioxidant activity, also hydroalchol extract has more phenol, but more flavonoid observed in ethanolic extract

کلیدواژه‌ها English

DPPH
β-carotene/linoleic
phenol content
flavonoid content
Different solvents
[1] Fazeli-nasab, B., Moshtaghi, N., & Forouzandeh, M. (2019). Effect of Solvent Extraction on Phenol, Flavonoids and Antioxidant Activity of some Iranian Native Herbs. scientific journal of ilam university of medical sciences, 27(3), 14-26.‌ (In Persian).
[2] Del Bano, M. J., Lorente, J., Castillo, J., Benavente-García, O., Del Rio, J. A., Ortuño, A., ... & Gerard, D. (2003). Phenolic diterpenes, flavones, and rosmarinic acid distribution during the development of leaves, flowers, stems, and roots of Rosmarinus officinalis. Antioxidant activity. Journal of agricultural and food chemistry, 51(15), 4247-4253.‌
[3] Medini, F.; Fellah, H.; Ksouri, R.; Abdelly, C. (2014) “Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for science 8(3) 216-224, 2014.
[4] Tomsone, L., Kruma, Z., & Galoburda, R. (2012). Comparison of different solvents and extraction methods for isolation of phenolic compounds from horseradish roots (Armoracia rusticana). World Academy of Science, Engineering and Technology, 64, 903-908.‌
[5] Nazzaro, M., Mottola, M. V., La Cara, F., Del Monaco, G., Aquino, R. P., & Volpe, M. G. (2012). Extraction and characterization of biomolecules from agricultural wastes. Chemical Engineering Transactions, 12, 1164-1169‌.
[6] Esmaeilzadeh Bahabadi, S., & Yosefzaei, F. (2018). Effect of different solvents on extraction of phenolic and flavonoid compounds and antioxidant activity of Citrullus colocynthis. Food Science and Technology 74(15) 313-320. (In Persian)
[7] Kiokias, S., Varzakas, T., & Oreopoulou, V. 2008. In vitro activityof vitamins, flavanoids, and natural phenolic antioxidants against the oxidative deterioration of oil-based systems Critical Reviews in Food Science and Nutrition, 48, 78–93.
[8] Shahidi, F. and Ambigaipalan, P. (2015). Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects–A review. Journal of functional foods, 18, 820-897.
[9] Ghahraman, A. 1993. Cromophytes of Iran (Plant Systematics), Volume II, Tehran University Press (In Persian)
[10] Tepe, B.; Sokmen, M.; Akpulat, H.A.; Sokmen, A.(2006). Screening of the antioxidant potentials of six Salvia species from Turkey, Food Chemistry, 97, 200-204,
[11] Li, X.; Wang, Z.(2009) Chemical composition, antimicrobial and antioxidant activities of the essential oil in leaves of Salvia miltiorrhiza Bunge, Journal of Essential Oil Research, 5, 476-488,.
[12] Amiri, H. 2009. The in vitro antioxidative properties of the essential oils and methanol extracts of Satureja macrosiphonia Bornm. J. Natural. Product. Research. 25, 232-243.
[13] Karamian, R.; Azizi, A.; Asadbegy, M; Pakzad, R.( 2014) Essential oil composition and antioxidant activity of the methanol extracts of three Phlomis species from Iran Journal of Biologically Active Products from Nature, 4, 343-353.
[14] Do, Q.D.; Angkawijaya, A.E.; Tran-Nguyen, P.L.;Huynh, L.H.; Soetaredjo, F.E.; Ismadji, S. and Ju, Y.H.(2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of food and drug analysis 22(3) 296-302
[15] Hossain, M. A., Shah, M. D.( 2015). A study on the total phenols content and antioxidant activity of essential oil and different solvent extracts of endemic plant Merremia borneensis. Arabian Journal of Chemistry 8(1), 66-71.
[16] Hamedy Roby, M.H.; Sarhan, M.A.; Selim, Kh.A.H.; Khalel, Kh.I.(2013). Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts, Industrial Crops and Products, 43, 827– 831.
[17] Govahi, M., Ghorbani, F., Ranjbar, M., Rahaiee, S., & Azizi, H. (2019). Evaluation of Antioxidant and Antibacterial Activity, and Determination of Phenolic and Flavonoid Content of Aqueous and Methanolic Extracts of Scutellaria pekinensis. scientific journal of ilam university of medical sciences, 27(3), 91-100.‌ . (In Persian).
[18] Jamzad, M.; Hafez-Taghva, P.; Kazembakgloo, A.; Jamzad, Z.(2014). Chemical composition of essential oil, total flavonoid content and antioxidant activity of Salvia dracocephaloides Boiss. from Iran, Journal of Essential Oil Bearing Plants, 17(6), 1203-1210, 2014.
[19] Gálvez, M., Martín-Cordero, C., Houghton, P. J., & Ayuso, M. J. (2005). Antioxidant activity of methanol extracts obtained from Plantago species. Journal of Agricultural and Food Chemistry, 53(6), 1927-1933.‌
[20] Naczk, M., & Shahidi, F. (2006). Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. Journal of pharmaceutical and biomedical analysis, 41(5), 1523-1542.‌
[21] Alothman, M., Bhat, R., & Karim, A. A. (2009). Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chemistry, 115(3), 785-788.‌
[22] Amarowicz, R., Pegg, R. B., Rahimi-Moghaddam, P., Barl, B., & Weil, J. A. (2004). Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food chemistry, 84(4), 551-562.‌
[23] Samsamshariat SH. Extraction of effective components of herbal medicine determination and evaluation methods. 1th ed. Mani Pres Esfahan Publication. 1993; Iran P.12 -3. . (In Persian).