استفاده از نانو الیاف حاصل از الکتروریسی کیتوزان به عنوان نانوحامل عصاره درمنه دشتی: بررسی ویژگی‌ها و خاصیت ضد میکروبی

نویسندگان
میثم رضایی 1
مهدی آران 2
علی محمد امانی 3
محمد امین میری 4
داریوش رمضان 1
1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زابل
2 استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زابل، ایران
3 استادیار گروه نانو فناوری پزشکی، دانشکده علوم و فناوری‌های نوین پزشکی، دانشگاه علوم پزشکی شیراز
4 گروه صنایع غذایی، دانشکده کشاورزی، دانشگاه زابل
10.52547/fsct.18.112.323
چکیده
درمنه گیاهی با خاصیت دارویی فراوان می‌باشد که در درمان بیماری‌های پوستی استفاده می‌شود و دارای خواص ضد میکروبی بالایی می باشد. این تحقیق با هدف ساخت نانو الیاف حاوی عصاره درمنه دشتی و بررسی خاصیت ضد میکروبی نانوالیاف برای کنترل باکتری‌های سودموناس آئروژینوزا و استافیلوکوکوس اورئوس انجام شد. بدین منظور ابتدا عصاره گیاه درمنه دشتی و محلول الکتروریسی حاوی 2/0 گرم پلی اتیلن اکساید، 05/0 گرم کیتوزان و 5/1 میلی لیتر اسید استیک تهیه و سپس به وسیله‌ی الکتروریسی محلو‌ل‌ها، نانوالیاف تولید و مورد بررسی قرار گرفت. بارگذاری عصاره درمنه با غلظت 100میلی گرم بر میلی لیتر تاثیر معنی داری بر قطر الیاف نشان داد و میانگین قطر الیاف دارای عصاره (4/218 نانومتر) نسبت به الیاف فاقد عصاره (8/204 نانومتر) بیشتر بود. تنش در نقطه پارگی الیاف حاوی عصاره (MPa 04/3) به نسبت الیاف فاقد عصاره (MPa 46/3) پایین‌تر و همچنین تغییر طول در نقطه پارگی بالاتر (6/7 درصد) بودند. نانوالیاف حاوی عصاره مقاومت حرارتی بیشتری داشتند که به دلیل حالت کریستالی عصاره آرتمیزیا در نانوالیاف می باشد. نانو الیاف تولیدی حاوی عصاره درمنه برای هر دو باکتری مورد استفاده دارای خاصیت مهارکنندگی بود. در نانو الیاف حاوی 100 میلی گرم در میلی لیتر عصاره به طور کامل رشد باکتری کنترل شد و همچنین نانوالیاف دارای عصاره با غلظت 20 و 50 میلیگرم بر میلی لیتر، بر باکتری گرم منفی سودوموناس آئروژینوزا نسبت به باکتری گرم مثبت استافیلوکوکوس ارئوس اثر مهار کنندگی بهتری داشت. افزودن عصاره درمنه دشتی باعث بهبود ویژگی‌ها و خواص ضد میکروبی نانوالیاف کیتوزان شد.
کلیدواژه‌ها
الکتروریسی
انکپسولاسیون
گیاه دارویی
عصاره درمنه

موضوعات

عنوان مقاله English

Use of Electrospun Chitosan Nanofibers as Nanocarriers of Artemisia sieberi Extract: Evaluation of Properties and Antimicrobial effects

نویسندگان English

Meysam rezaei 1
Mehdi Aran 2
Ali Mohamad Amani 3
Mohammad amin Miri 4
Dariush Ramezan 1
1 Department of Horticulture, Faculty of Agriculture, University of Zabol
2 Assistant Professor, Department of Horticulture, Faculty of Agriculture, University of Zabol
3 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Shiraz
4 Department of Food Industry, Faculty of Agriculture, University of Zabol
چکیده English

Artemisia is an important medicinal plant which is widely used in the treatment of skin diseases and it has antimicrobial properties. This research was carried out with the aim of making nano-fibers with Artemisia sieberi Besser extract to study its antimicrobial properties against Pseudomonas aeruginosa and Staphylococcus aureus bacteria. Artemisia extract and electrospinning solution containing 0.2 g of polyethylene oxide, 0.05 g of chitosan and 1.5 ml of acetic acid were prepared and then nanofibers were produced by electrospinning of the solutions. Artemisia Extract loading with 100 mg/ml extract had a significant effect on the diameter of fibers and the average fber diameter with Artemisia extract (218.4 nm) compared to the fibers without extract (204.8 nm) were higher. The tensile stress at the tear point of the nanofibers with Artemisia extract (3.04 MPa) was lower than the fibers without the extract (3.46 MPa) and elongation at break was higher (7.6%).The nanofibers with the extract had more resistance temperature than non-extract fibers. This is due to the crystal state of Artemisia extract in the fibers. The nanofibers produced with Artemisia extract had inhibitory properties for both studied bacteria. Nanofibers with 100 mg/ml extract completely controlled the bacterial growth. Nanofibers with 20 and 50 mg/ml of extract had a better inhibitory effect on Pseudomonas aeruginosa than Staphylococcus aureus. The Addition of Artemisia extract improved the antimicrobial properties of chitosan nanofibers.

کلیدواژه‌ها English

Artemisia extract
Electrospinning
Encapsulation
Medicinal Plant
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