شناسایی ترکیبات شیمیایی و ارزیابی فعالیت ضدقارچی عصاره هیدرواتانولی میوه عناب بر کپک آسپرژیلوس فلاووس در دانه های ذرت آنزیم بری شده

نویسندگان
محمد ابراهیم ملکیان 1
انوشه شریفان 2
افشین آخوندزاده بستی 3
1 کارشناسی ارشد، گروه علوم و صنایع غذایی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 دانشیار گروه علوم و صنایع غذایی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 دکترای تخصصی، استاد گروه بهداشت و کنترل کیفی دانشکده دامپزشکی دانشگاه تهران.
10.52547/fsct.17.106.173
چکیده
امروزه مطالعات به منظور بهره گیری از خواص ضد میکروبی گیاهان به جای استفاده از نگهدارنده های سنتتیک در حال گسترش است. گیاه عناب با نام علمی Ziziphus jujube Mill. از خانواده رامناسه و بومی نواحی مرکزی فلات ایران است. میوه، برگ و ریشه این گیاه دارای خواص دارویی و سلامتی بخش می باشند. در این پژوهش عصاره گیری از میوه عناب خشک، به روش خیساندن به وسیله حلال هیدرواتانلی انجام گرفت و سپس عصاره به روش انجمادی، خشک شده و راندمان عصاره گیری از پودر میوه عناب، اندازه گیری شد. ترکیبات شیمیایی تشکیل دهنده عصاره با روش GC-MS تعیین شدند و پس از اندازه گیری راندمان استخراج وبرخی پارامتر های فیزیکیوشیمیایی عصاره شامل رطوبت ، فعالیت آبی، چربی، خاکستر، بریکس، در ادامه حداقل غلظت بازدارنده و حداقل غلظت کشنده عصاره اتانولی میوه عناب در برابر کپک Aspergillus fulvous (PTCC 5004)، به روش رقیق کردن در محیط مایع عصاره ارزیابی شد. هم چنین غلظت های مختلف عصاره بر نمونه های ذرت اسپری شده و پس از قرار دادن یک دیسک حاوی سوسپانسیون کپکی در مرکز هر پتری دیش، خاصیت ضد کپکی عصاره بر روی دانه های ذرت در طی مدت 7 روز ارزیابی گردید. نتایج نشان دادند که عصاره اتانولی میوه عناب دارای خاصیت ضد کپکی بوده و حداقل غلظت بازدارنده عصاره در محیط کشت آزمایشگاهی 350 میکروگرم بر میلی لیتر تعیین شد. در غلظت های کم تر 1000 میکروگرم بر میلی لیتر خاصیت کشندگی از عصاره مشاهده نشد. هم چنین بازدارندگی عصاره در محیط دانه های ذرت آنزیم بری شده، در غظتهای 5000 -2000 میکروگرم بر میلی لیتر ارزیابی شد. نتایج به دست آمده نشان داد که عصاره اتانولی میوه عناب دارای خاصیت ممانعت کنندگی در برابر کپک آسپرژیلوس فلاووس بوده اما فاقد خاصیت غیر فعال سازی اسپور کپک مذکور است.
کلیدواژه‌ها
آسپرژیلوس فلاووس
ذرت
عصاره هیدروالکلی
عناب
فعالیت ضدکپکی

موضوعات

عنوان مقاله English

Identification compounds fruit of Ziziphus jujube Mill hydroethanoly extract and evaluation of Its antifungal effect on blanched corn grains

نویسندگان English

MohammadEbrahim Malekian 1
Anousheh Sharifan 2
afshin akhondzadeh basti a 3
1 no affilation
2 Islamic Azad University, Science and Research
3 Tehran University
چکیده English

Nowadays, studies have been developing in order to use antimicrobial properties of plants instead of using synthetic preservatives. Jujube is one of the famous herbs. Jujube (Ziziphus jujube. Mill) belongs to family of Ramnaseh and native to the central areas of the Iran plateau. The fruit, leaves and root of this plant have medicinal and wellness properties. In this study, evaluated some physico-chemical of jujube extract (Extraction yield, Moisture, water activity, Fat content, Ash, Brix) and its effect on the mold of Aspergillus flavus. Extraction of dried and Ground jujube fruit was done by soaking with Hydroethanol solvent. Chemical composition of the extract was determined by GC-MS method. So the evaluated minimum inhibitory concentration (MIC) and the minimum fungal concentration (MFC) of extract against Aspergillus fulvous (PTCC 5004) by the dilution method in a liquid medium. In the next step, different concentrations of the extract were sprayed on corn grains and after placing a disk containing suspension of Mold spores in each petri dish. Growth of mold on corn grains was measured during 7 days. The results showed that the hydroethanolic extract of jujube fruit has an anti-mold effect against Aspergillus fulvous and the minimum inhibitory concentration of extract in the culture medium was determined 350 μg / ml and there was not observed any lethality at concentrations less than 1000 μg / ml. The minimum inhibitory concentration of the extract in the blanched corn grain medium was 5000 μg / ml. Therefore, the hydroethanolic extract of jujube fruit has an inhibitory effect on Aspergillus flavus but does not have the inactivation effect on this mold and its spores.

کلیدواژه‌ها English

Aspergillus flavous
Ziziphus jujuba
Hydroethanolic extract
Anti fungal
Maize
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