مقایسه میزان فعالیت آنتی اکسیدانی در دو گیاه ریحان (Ocimum basilicum)و گل حنا (Impatiens walleriana) و بررسی خواص ضد میکروبی و ضد سرطانی آنها بر روی سرطان تخمدان

نویسندگان
پریچهر حناچی 1
شقایق صالحی زاده 1
ریحانه رمضانی 2
روشنک زرین قلمی 1
1 گروه بیوتکنولوژی ،بخش بیوشیمی، دانشکده علوم زیستی ، دانشگاه الزهرا ، تهران ، ایران
2 گروه پژوهشی بیومدیکال ، بخش پژوهشکده زنان ، دانشگاه الزهرا ، تهران ، ایران
10.52547/fsct.17.106.95
چکیده
سرطان تخمدان یکی از کشنده ترین سرطان ها در خانم ها محسوب می شود و باعث ایجاد سلول های غیرطبیعی سرطانی میشود که این سلول ها توانایی گسترش و حمله به سایر قسمت های بدن را دارا میباشد. متابولیت های ثانویه از لحاظ ساختاری و شیمیایی بسیار متنوع می باشند و نقش مهمی در درمان انواع سرطان ایفا می کنند. در این مطالعه عصاره دو گیاه تحت تأثیر سه حلال ( آب ، اتانول 80 % و متانول 80 % ) روش عصاره گیری خیساندن گرفته شد و با استفاده از روش فولین سیوکالتو ، میزان ترکیبات فنلی و با روش های DPPH (دی فنیل پیکریل هیدرازیل) و FRAP ( توانایی احیای آهن ) فعالیت آنتی اکسیدانی دو گیاه سنجیده شد. سپس از بین حلال ها، حلال آب که دارای بیشترین خاصیت آنتی اکسیدانی بود جهت بررسی خواص ضد سرطانی بر روی سلول های سرطان تخمدان انتخاب گردید که کشنده ترین عصاره مربوط به عصاره آبی گیاه ریحان در 72 ساعت با میزان IC50 برابر با 001/0 ± 105/1 میلی گرم بر میلی لیتر بوده است. میزان ترکیبات فنلی در دو گیاه تحت تأثیر حلال متانول 80 % بیشترین میزان بود و بیشترین فعالیت آنتی اکسیدانی نیز در دو گیاه در حلال آب مشاهده شد . در بررسی خاصیت ضد میکروبی به روش انتشار دیسکی ، این خاصیت فقط در عصاره های اتانولی دو گیاه دیده شد. در سنجش MIC (Minimum Inhibitory Concentration) و(Minimum Bactericidal Concentration) MBC عصاره های اتانولی و متانولی گیاه ریحان و در گل حنا در عصاره های آبی و اتانولی دارای بیشترین اثر بازدارندگی و همچنین کشندگی بودند.
کلیدواژه‌ها
ریحان
گل حنا
خاصیت ضد سرطانی
خاصیت ضد میکروبی
خاصیت آنتی اکسیدانی

موضوعات

عنوان مقاله English

Comparison of antioxidant and anti-bacterial activities of Ocimum basilicum and Impatiens walleriana and their anticancer properties on SKOV-3 cancer cell line

نویسندگان English

Parichehr Hanachi 1
Shaghayegh Salehizadeh 1
Reihaneh Ramezani 2
Roshanak Zarringhalami 1
1 Department of Biochemistry, Faculty of Biological Sciences, Alzahra University, Tehran, IRAN
2 Biomedical Research group, Women’s Research Center, Alzahra University, Tehran, IRAN
چکیده English

Abstract

Ovarian cancer is one of the deadliest cancers in women causing abnormal cancerous cells that can spread and metastasize to other parts of the body. Secondary metabolites are highly variable in terms of structural and chemical properties and they are very important for plants survival. In this study, the extracts of two plants using three different solvents (water, ethanol 80% and methanol 80%) and water bath extraction method were obtained. phenolic compounds of extracts were determined by Folin Sioculto method. DPPH (2, 2-Diphenyl-1-picrylhydrazyl) and FRAP (Ferric reducing ability of plasma) methods were performed for determination of antioxidant properties. The amount of phenolic compounds in two plants was higher in 80% methanol solvent and the most antioxidant activities were observed in water solvent of extracts. Water solvent had the highest antioxidant activity and was selected to investigate the anti-cancer properties of extracts on ovarian cancer cells. Water extract of Ocimum basilicum within72 hours had the highest cytotoxic effect with an IC50 value of 1.105 ± 0.001 mg / ml. In study of antimicrobial activity by disc diffusion method, the highest antibacterial properties were only in ethanol extracts of two plants. In the measurement of MIC and MBC, ethanol and methanol extracts of Ocimum basilicum and water and ethanol extracts in Imatiens walleriana had the most inhibitory and bactericide effects.

کلیدواژه‌ها English

Anticancer
Antimicrobial
Antioxidant
Impatiens walleriana
Ocimum basilicum
[1] Noori-Daloii, M. R., & Rashvand, Z. 2010. Molecular genetics and gene therapy in ovarian cancer . Ofogh-e-Danesh. GMUHS Journal. 16(3) : 5 – 20
[2] Sholiche W. (20170 . Phytochemical screening and antioxidant activity of ethanolic extract and ethyl acetate fraction from basil leaf (Ocimum basilicum L.) by DPPH radical scavenging method . Material science and Engineering . 1 – 11 .
[3] Hanachi P, Zarringhalami R, Tamijani RR. Investigation of Antioxidant Properties of Polygonatum orientale Desf and Tilia dasystyla Extracts by Different Methods and Solvents. Hormozgan Medical Journal. 2018 Dec 31;22(4).
[4] Chirag, P., Tyagi, S., Halligudi, N., Yadav, J., Pathak, S., Singh, S. P. and Shankar, P. 2013.Antioxidant activity of herbal plants: a recent review .Journal of Drug Discovery and Therapeutics. 1(8) : 1 – 8 .
[5] Abou El-soud n. , Deabes M. , Abou El-Kassem L. and Khall M. (2015). Chemical composition and antifungal activity of Ocimum basilicum L. essential oil. Medical Sciences Journal. 3(3): 374-379.
[6] Mahmoud GI. Biological effects, antioxidant and anticancer activities of marigold and basil essential oils. Journal of Medicinal Plants Research. 2013 Mar 10;7(10):561-72.
[7] Fitsiou E, Mitropoulou G, Spyridopoulou K, Tiptiri-Kourpeti A, Vamvakias M, Bardouki H, Panayiotidis MΙ, Galanis A, Kourkoutas Y, Chlichlia K, Pappa A. Phytochemical profile and evaluation of the biological activities of essential oils derived from the Greek aromatic plant species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita. Molecules. 2016 Aug;21(8):1069.
[8] Mandle L. , Warren D. , Hoffmann N. , Peterson D. , Schmitt A. and Wettberg E. (2010). conclusions about niche expansion in introduced Impatiens walleriana populations depend on method of analysis.
[9] Kang, S.-N., Goo, Y.-M., Yang, M.-R., Hag Ibrahim, R., Cho, J.-H., Kim, I.-S., and Lee, O.-H. 2013. Antioxidant and antimicrobial activities of ethanol extract from the stem and leaf of Impatiens balsamina L. (Balsaminaceae) at different harvest times . Journal of Molecules.18(6) : 6356 – 6365 .
[10] Kumar S. and Pandey A. (2013). Chemistry and biological activities of flavonoids: an overview. The ScientificWorld Journal.
[11] Oliveira, J. L. T. M. d., M. d. F. M. Diniz, E. d. O. Lima, E. L. d. Souza, V. N. Trajano and B. H. C. Santos .(2009) . Effectiveness of Origanum vulgare L. and Origanum majorana L. essential oils in inhibiting the growth of bacterial strains isolated from the patients with conjunctivitis. Brazilian Archives of Biology and Technology .52(1): 45-50 .
[12] Motalleb G., Hanachi P., Kua SH., Fauziah O. and Asmah R. (2005). Evaluation of phenolic content and total antioxidant activity in Berberis vulgaris fruit extract. Journal of Biological Science. 5(5): 648-653.
[13] Biju, J., Suliaman, C., Satheesh, G.and Reddy, V. (2014). Total phenolics and flavonoids in selected medicinal plants from Kerala. International Journal of Pharmacy and Pharmaceutical Sciences. 14(1) : 406 – 408 .
[14] Safafar H. , Wagene J. , Moller P. and Jacson C. (20150 . Carotenoids, Phenolic Compounds and Tocopherols Contribute to the Antioxidative Properties of Some Microalgae Species Grown on IndustrialWastewater . Mrine drugs journal . 13(12): 7339 – 7356 .
[15] Kaushik, A., Jijta, C., Kaushik, J. J., Zeray, R., Ambesajir, A.and Beyene, L. (2012). FRAP ( Feric reducing ability of plasma ) aasay and effect of Dilazium esculentum (Retz ) Sw.
[16] Chakraborty, M. and A. Mitra .(2008). The antioxidant and antimicrobial properties of the methanolic. extract from Cocos nucifera mesocarp. Food Chemistry 107(3): 994-999.
[17] Mazzola, P. G., A. F. Jozala, L. C. d. L. Novaes, P. Moriel and T. C. V. Penna .(2009). Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents. Brazilian Journal of Pharmaceutical Sciences 45(2): 241-248.
[18] Hanachi P. , Kua S.H. , Asmah R. , Motalleb G. and Fauziah O. 2006 . Cytotoxic effect of Berberis vulgaris fruit extract on the proliferation of human liver cancer cell line ( HepG2 ) and its antioxidant properties. International journal of cancer research .
2(1) : 1 – 9 .
[19] Abdkhani S. and solooki M. (2016) . Changes in phenolic compounds, anthocyanins and antioxidant enzymes in different stages of Ocimum basilicum growth under the influence of growth reguators . Medicinal plants J . 15 (2): 164 – 175 .
[20] Chirinos R. , Rogez H. , Campos D. , Pedreschi R. and LarondelleY. (2007) . Optimization of extract conditions of antioxidant phenolic compound from mashua(Tropaeolum tuberosum Ruiz and Pavon) tubers.Separation and purification technology . 55(2):217 – 225.
[21] Sameie A. , Tabatabie-Yazdi F. and Mazaheri Tehrani M.(2018) . An investigation into the antioxidant activity, phenolic compounds, antimicrobial effect and interaction of the essential oils of Curcuma longa and Ocimum basilicum on some pathogenic bacteria. Food Science and Technology journal.15(74) : 99 – 107.
[22] Tooryan , F., & Azizkhani, M. (2017). Antioxidant effect of the aerial parts of basil (Ocimum basilicum) and clary sage (Salvia sclarea) essential oils in Iranian white cheese . Iranian Food Science and Technology Journal.13(2) : 346 – 362 .
[23] Juliani H.R. and Simon J.E. 2002 . Antioxidant Activity of Basil . Trends in new crops and new uses. 575 – 579 .
[24] Morrissey, J. P. and A. E. Osbourn (1999). Fungal resistance to plant antibiotics as a mechanism of pathogenesis. Microbiology and Molecular Biology Reviews 63(3): 708-724.
[25] Shekouh Saremi E. , Habibi Najafi M.B. , Hadad Khodaparast M.H. and Baheini M. (2018) .Effect of extraction methods on phenolic content and antimicrobial properties of Pimpinella affinis leaf . Research Journal .14(1) : 59 – 68 .
[26] Dorosti, N., Zarabi, S., Ahmadi, S., Rostami, R., & Rashidi Pour, M. (2017). " Anticancer activity evaluation of methanolic extract of Allium Jesdianum and Nectaroscordeum Coelzi against HeLa and K562 cell lines". Journal of Yafteh.1(71) : 31 – 41.
[27] Kehkashan Arshad, Q., Ahsana , D., Bina S. , S., Nurul, K., Huma, A., Shakil, A., Sabira, B. 2010. Anticancer activity of Ocimum basilicum and the effect of ursolic acid on the cytoskeleton of MCF-7 human breast cancer cells.Journal of Letters in Drug Design & Discovery. 7(10) : 726 – 736 .
[28] Zarlaha, A., Koupkoumelis, N., & Stanojkovik, T. 2014. Cytotoxic activity of essential oil and extract of Ocimum basilicum against human carcinoma cells. Molecular docking study of isoeugenol as a potent cox and lox inhibitor. Digest Journal of Nanomaterials and Biostructures . 9(3) : 907 – 917