شناسایی ترکیبات شیمیایی، بررسی توانایی رادیکال گیرندگی و خواص ضد میکروبی عصاره بذر گیاه عدس الملک

نویسندگان
علی بهنام نیک
محسن وظیفه دوست
زهره دیدار
بهاره حاجی رستم لو
گروه علوم و صنایع غذایی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران
چکیده
برخی از گیاهان دارای ترکیبات آنتی­اکسیدانی و خواص ضد میکروبی بوده و از آن­ها می­توان به عنوان ترکیبات فراسودمند در صنعت استفاده نمود، هدف از این مطالعه بررسی قدرت رادیکال ­گیرندگی، ضد میکروبی و شناسایی ترکیبات عصاره بذر گیاه عدس­الملک می­باشد. در این مطالعه ابتدا عصاره­گیری به روش ترکیبی خیساندن و اولتراسوند (50 هرتز) انجام گردید. سپس شناسایی ترکیبات با دستگاه GC/MS انجام گردید و قدرت رادیکال گیرندگی به وسیله DPPH تعیین گردید. اثر ضد میکروبی عصاره در برابر سویه­های اشرشیا کلی، لیستریامونوسیتوژنز و کاندیدا آلبیکنس به روش انتشار دیسک تعیین گردید. 14 نوع ترکیب شناسایی گردید که جمعا 58/98 درصد ترکیبات عصاره را شامل می­شدند و بیش­ترین ترکیبات را Mome Inositol (30.55%)، cis-9-Octadecenoic acid (20.44%) تشکیل دادند. بیشترین قدرت رادیکال گیرندگی مربوط به غلظت ppm600 عصاره (91.6 درصد) بوده است. همه غلظت­های مختلف عصاره به غیر از غلظت 0.25 میلی­گرم بر میلی­لیتر بر باکتری لیستریا مونوسیتوژنز قدرت مهارکنندگی داشته­اند و تمام غلظت­های عصاره بر باکتری اشرشیا­کلی اثر مهارکنندگی داشته­اند و بیش­ترین اثر مربوط به غلظت 0.5 میلی­گرم بر میلی­لیتر(12.98 میلی­متر) بود. همچنین هیچ یک از غلظت­ها اثر مهارکنندگی بر روی قارچ کاندیدا آلبیکنس نداشته­اند. نتایج نشان داد با افزایش غلظت عصاره اثر بازدارندگی آن در برابر سویه­ها به شکل موثری افزایش می­یابد. با توجه به قدرت آنتی­اکسیدانی مناسب و خاصیت ضد میکروبی عصاره این گیاه می­توان از آن در صنایع غذایی و دارویی استفاده نمود.
کلیدواژه‌ها
آنتی اکسیدان
ضد میکروبی
کروماتوگرافی
عدس الملک

موضوعات

عنوان مقاله English

Identification of Chemical Compounds, radical scavenging activity and Antimicrobial Properties of Seed Extract of Securigera securidaca L

نویسندگان English

Ali Behnam nik
Mohsen Vazifedoost
Zohreh Didar
Bahareh Hajirostamloo
, Department of Food Science and Technology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
چکیده English

Some plants have antioxidant compounds and antimicrobial properties and can be used as fractional compositions in the industry. This study aimed to investigate the radical scavenging and antimicrobial properties as well as the determination of the compounds in the seed extract of Securigera securidaca. The plant seeds were extracted by maceration method using ultrasound (50 Hz) and dried extract in this study. Then, the compounds were identified by GC/MS device and the radical scavenging capacity was determined by DPPH. The antimicrobial properties of the extract were determined by disc-diffusion method against Escherichia coli, Listeria monocytogenes and Candida albicans strains. Fourteen (14) compounds were identified, containing 98.58% of total extract compositions, and the highest percentage was related to Mome Inositol (30.55%) and cis-9-Octadecenoic acid (20.44%). The highest radical scavenging capacity was observed at the concentration of 600 ppm of extract (91.6%). Different concentrations of the extract rather than the concentration of 0.25 mg/ml inhibited L. monocytogenes and all concentrations of extract had inhibitory effect on E. coli and the most effect was related to the concentration of 0.5 mg/ml (12.98 mm). None of the concentrations had an inhibitory effect on C. albicans. The results showed that the extract inhibitory effect increased on the strains effectively by increasing the concentration of extract. The plant extract can be used in the nutraceutical industries due to its appropriate antioxidant activity and antimicrobial properties.

کلیدواژه‌ها English

: antioxidants
Antibacterial
Chromatography
Securigera securidaca
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