اصالت‌سنجی آب‌لیموهای مصرفی استان گلستان با استفاده از کروماتوگرافی مایع جفت شده با آشکارساز جرمی

نویسندگان
1 استادیار گروه شیمی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
2 استادیار مؤسسه تحقیقات گیاه پزشکی کل کشور، تهران، ایران
3 مدیر عامل آزمایشگاه علوم حیاتی فاروق، تهران، ایران
چکیده
آب‌لیموهای صنعتی ممکن است دارای افزودنی‌های غیرمجاز بوده که منجر به بیماری‌های مختلفی در افراد جامعه می‌شوند. درنتیجه، استفاده از روش‌های کارا و مؤثر جهت شناسایی آب‌لیموهای تقلبی بسیار حائز اهمیت است. در تحقیق حاضر برای اولین بار در کشور، تشخیص تقلب در آب‌لیموهای صنعتی در استان گلستان از طریق آنالیز اسیدهای آلی شامل اسیدسیتریک، اسید ایزوسیتریک، اسید مالیک و همچنین تعیین نسبت بین اسیدسیتریک و اسید ایزوسیتریک با استفاده از کروماتوگرافی مایع جفت شده با آشکارساز جرمی ارائه شده است. صحت روش ارائه‌شده، از طریق آنالیز نمونه‌های آب‌لیموی طبیعی تأیید شد. حد تشخیص و حد اندازه‌گیری کمی برای اسیدسیتریک، اسید ایزوسیتریک و اسید مالیک به ترتیب 2 و 5، 0/02 و 0/05، 0/2 و 0/5 میلی‌گرم بر لیتر به دست آمد. نمونه‌های آب‌لیموی صنعتی از برندهای تجاری پرمصرف موجود در سطح عرضه انتخاب شدند و شناسایی نمونه‌های تقلبی از طریق مقایسه مقادیر اسیدهای آلی با مقادیر مجاز تعیین‌شده توسط استاندارد اروپا، انجام شد. نتایج به‌دست‌آمده نشان داد که روش پیشنهادی بسیار مؤثر و حساس در تشخیص نمونه‌های تقلبی آب‌لیمو صنعتی می‌باشد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Authentication of consumed lemon juice in Golestan province using liquid chromatography-mass spectrometry

نویسندگان English

Mehdi Maham 1
R. Karami-Osboo 2
M.H. Shojaee AliAbadi 3
1 Associate Professor, Department of Chemistry, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 Associate Professor of Iranian Research Institute of Plant Protection, Tehran, Iran
3 Faroogh Life Sciences Research Laboratory No. 96.2, Tehran 1457844393, Iran
چکیده English

Industrial lemon juice may contain unauthorized additives that can lead to various diseases in society. As a result, it is highly important to use effective methods to identify the fake lemon juice. In this study, for the first time in our country, detection of cheating in industrial lemon juice in Golestan province is presented by analyzing organic acids, including citric acid, isocytic acid, malic acid, and also determining the ratio between citric acid and isocytic acid using liquid chromatography-mass spectrometry. The accuracy of the proposed method was confirmed by analyzing natural lemon juice samples. The limits of detection and quantification were 2 and 5, 0.02 and 0.05, 0.2 and 0.5 mg L-1 for citric acid, isocytic acid, malic acid, respectively. Industrial lemon juice samples were selected from commercially available brands, and fake samples were detected by comparison of the amounts of organic acids with the authorized values set by the European Standard. The results showed that the proposed method is very effective and sensitive in detecting cheating samples of industrial lemon juice.

کلیدواژه‌ها English

Lemon juice
Organic acids
Liquid chromatography-mass spectrometry
[1] Ahmadi, K., Gholizadeh, H., Ebadzadeh, H.R., Hatami, F., Hosseinpour R., Ebadshah, H., Rezaei M.M., Fazli Estabragh M. (1396). Agricultural Statistics, Horticultural Products, 3, 1−231.
[2] Food And Agricultural Organization of the United Nations: Economic And Social Department: The Statistical Division.
[3] Saeidi, I., Hadjmohammadi, M.R., Peyrovi, M., Iranshahi, M., Barfi, B., Babaei, A.B., Dust, A.M. (2011). HPLC determination of hesperidin, diosmin and eriocitrin in Iranian lime juice using polyamide as an adsorbent for solid phase extraction. Journal of Pharmaceutical and Biomedical Analysis, 56(2), 419– 422.
[4] Vekiari, S.A., Protopapadakis, E.E., Papadopoulou, P., Papanicolaou, D., Panou, C., Vamvakias, M. (2002). Composition and seasonal variation of the essential oil from leaves and peel of a cretan lemon variety. Journal of Agricultural and Food Chemistry, 50(1), 147−153.
[5] Vekiari, S.A., Protopapadakis, E.E., Papadopoulou, P., Papanicolaou, D., Panou, C., Vamvakias, M. (2002). Composition and seasonal variation of the essential oil from leaves and peel of a cretan lemon variety. Journal of Agricultural and Food Chemistry, 50, 147−153.
[6] Moshonas, M.G., Shaw, P.E. (1972). Analysis of flavor constituents from lemon and lime essence. Journal of Agricultral Food Chemistry, 20, 1029−1030.
[7] Scherer, R., Rybka, A.C.P., Ballus, C.A., Meinhart, A.D., Filho, J.T., Godoy, H.T. (2012). Validation of a HPLC method for simultaneous determination of main organic acids in fruits and juices. Food Chemistry, 135(1), 150–154.
[8] Camara, M.M., Diez, C., Torija, M.E., Cano. M.P. (1994). HPLC determination of organic acids in pineapple juices and nectars. European Food Research and Technology, 198(1), 52−56.
[9] Uckoo, R.M., Jayaprakasha, G.K., Nelson, S.D., Patil. B.S. (2011). Rapid simultaneous determination of amines and organic acids in citrus using high-performance liquid chromatography. Talanta, 83(3), 948–954.
[10] Anonymous. (2016). AIJN - European Fruit Juice Association. Reference Guideline for Lime Juice. 1−4.
[11] Bylund, D., Norstrom, S.H., Essen, S.A., Lundstrom, U.S. (2007). Analysis of low molecular mass organic acids in natural waters by ion exclusion chromatography tandem mass spectrometry. Journal of Chromatography A, 1176(1−2), 89–93.
[12] Suarez-Luque, S., Mato, I., Huidobro, J.F., Simal-Lozano, J., Sancho, M.T. (2002). Rapid determination of minority organic acids in honey by high-performance liquid chromatography. Journal of Chromatography A, 955(2), 207–214.
[13] Wang, P., Zhou, R. (2006). Determination of organic acids exuded from plant roots by high performance liquid chromatography. Chinese Journal of Chromatography, 24(3), 239–242.
[14] Bogdanov, S., Charrière, J.D., Imdorf, A., Kilchenmann, V., Fluri, P. (2002). Determination of residues in honey after treatments with formic and oxalic acid under field conditions. Apidologie, 33(4), 399–409.
[15] Murtaza, M.A., Rehman, S.U., Anjum, F.M., Huma, N., Tarar, O.M., Mueen-Ud-Din, G. (2012). Organic acid contents of buffalo milk cheddar cheese as influenced by accelerated ripening and sodium salt. Journal of Food Biochemistry, 36(1), 99–106.
[16] Suzzi, G., Sacchetti, G., Patrignani, F., Corsetti, A., Tofalo, R., Schirone M., Fasoli, G., Gardini, F., Perpetuini, G., Lanciotti, R. (2014). Influence of pig rennet on fatty acid composition, volatile molecule profile, texture and sensory properties of Pecorino di Farindola cheese. Journal of the Science of Food and Agriculture, 95(11), 2252−2263.
[17] Laube, H., Boden, J., Schneider, R. (2017). Capillary electrophoresis method for the analysis of organic acids and amino acids in the presence of strongly alternating concentrations of aqueous lactic acid. Bioprocess and Biosystems Engineering, 40(7), 981−988.
[18] Mato, I., Suarez-Luque, S., Huidobro, J.F. (2005). A review of the analytical methods to determine organic acids in grape juices and wines. Food Research International, 38(10), 1175–1188.
[19] Mimmo, T., Ghizzi, M., Marzadori, C., Gessa, C.E. (2008). Organic acid extraction from rhizosphere soil: effect of field-moist, dried and frozen samples. Plant and Soil, 312(1−2), 175–184.
[20] Shen, H., Yan, X., Zhao, M., Zheng, S., Wang, X. (2002). Exudation of organic acids in common bean as related to mobilization of aluminum- and iron-bound phosphates. Environmental and Experimental Botany, 48(1), 1–9.
[21] Kotani, A., Miyaguchi, Y., Tomita, E., Takamura, K. (2004). Determination of organic acids by high-performanchromatography with electrochemical detection duribrewing. Journal of Agricultural and Food Chemistry, 52(6), 1440–1444.
[22] Aktas, A.H., Şen, S., Yılmazer, M., Cubuk, E. (2005). Determination of carboxylic acids in apple juice by RP HPLC. Iranian Journal of Chemistry and Chemical Engineering, 24(1), 1−6.
[23] Yoshida, H., Mizukoshi, T., Hirayama, K., Miyano, H. (2007). Comprehensive analytical method for the determination of hydrophilic metabolites by high-performance liquid chromatography and mass spectrometry. Journal of Agricultural and Food Chemistry, 55(3), 551−560.
[24] Yoshida, H., Yamazaki, J., Ozawa, S., Mizukoshi, T., Miyano. H. (2009). Advantage of LC-MS Metabolomics Methodology Targeting Hydrophilic Compounds in the Studies of Fermented Food Samples. Journal of Agricultural and Food Chemistry, 57(4), 1119–1126.
[25] Taghizadeh, M., Asemi, Z., Shakeri, H., Gholsorkhi, F., Takhtfiroozeh, S.M. (2014) The Sensitivity and specifity of spectrophotometer and polarimeter methodes in the detection of fraud of produced lemon juice in Iran. Medical Journal of Tabriz University of Medical Sciences and Health Services. 36(5), 16−21.
[26] Pirsa, S., Alizadeh, M., Faraji, N., garage, S. (2018). Determination of industrial lemon juice adulteration in organic .lemone juice by statistical analysis and physicochemical characteristic. Food Science and Technology, 14(73), 255−263.
[27] Róth, E., Berna, A., Beullens, K., Yarramraju, S., Lammertyna, J., Schenk, A. Nicolaï, B. (2007). Postharvest quality of integrated and organically produced apple fruit. Postharvest Biology and Technology, 45(1), 11−19.