ارزیابی ترکیبات شیمیایی و فعالیت ضدباکتریایی اسانس گیاهان خوشاریزه Echinophora cinerea Boiss و چای کوهی Stachys lavandulifolia Vahl در شرایط آزمایشگاهی

The aim of this study was to investigate the antibacterial activities of Echinophora cinerea and Stachys lavandulifolia Vahl essential oils cultivated in Lorestan province against some food pathogens. The aerial parts of these plants were collected from the mountains around Khorramabad. Their essential oils were extracted by Clevenger and their chemical composition analyzed by GC/MS. The antimicrobial activities were determined by disk diffusion and micro-dilution methods against Shigella dysenteriae, Escherichia coli, Staphylococcus aureus and Bacillus cereus. Thirty- nine and fifty-eight components were identified inEchinophora and Stachys essential oils, respectively. α-phellandrene, p-cymene, carvacrol and α-pinene were the main compounds in Echinophora essential oil and thymol, trans caryophyllene, β-phellandrene, spathulenol and caryophyllene oxide were the major components of Stachys essential oil. Findings showed antibacterial properties of Echinophora and Stachys. They acted against S. aureus with diameters of the inhibition zones of 34.5 and 22.8 mm, respectively. The minimum inhibitory concentrations (MIC) and MBC of Echinophorafor E.coli were 4.6, and 18.75 mg/mL. Results demonstrated that MIC of Stachys for all bacteria was 2.3 mg/mL. In addition, MBC of Stachys on E. coli and S. dysanteria was observed when it was adjusted on 2.3 mg/mL.