اندازه گیری فولات در آرد غنی شده به روش استخراج سه آنزیمی و سنجش میکروبی

Nowadays because of several problems and diseases that treat human health, considering special attention to suitable nutrition and achieving confidence about safe and rich content foods is a very important and unavoidable issue. Folic acid is one of required vitamins for our body that must be provided sufficiently. So there are numerous efforts has been done for more reliable measuring of this vitamin in foods. There are several methods available for measuring folic acid. In this research, two methods have been investigated. The main mentioned method was tri-enzyme extraction and microbiological assay which performed by α-amylase, protease, conjugase and a folate dependent microorganism which was Lactobacillus rhamnosus. Second method was High performance liquid chromatography that is performed in order to proving reliability and accuracy of the first method. Final results of this research showed that tri-enzyme extraction and microbiological assay has more accurate results comparing to high performance liquid chromatography. The reason is that the amount of folic acid detected by microbiological assay is about 50% more than those detected by High performance liquid chromatography. The main reason for existence of this difference in indicated values is due to complete extraction in microbiological assay. We concluded that tri-enzyme extraction and microbiological assay has more efficiency than High performance liquid chromatography to determine accurate amount of sensitive vitamins such as folic acid. In addition, by this method the amount of total folate can be determined.