عنوان مقاله English
Antifungal drugs have an important role for treatment of fungal skin diseases of livestock and prophylaxis. These compounds belong to the different chemical groups but Imidazole group is one of the most important groups.
In this study 60 pasteurized milk samples were analyzed by liquid-liquid microextraction and high performance liquid chromatography. And the efficiency of solid phase extraction and high performance chromatography-UV spectroscopy was evaluated in the extraction and determination of Ketoconazole and Clotrimazole analytes in milk.
In this research, after full validation, detection and quantitation limits were 0.01 μg.ml-1 and 0.1 μg.mL-1 for Ketoconazole and Clotrimazole. The linear range of calibration was relatively wide (0.01-100 μg.ml-1). The relative standard deviation was 0.1-7.5% for Ketoconazole and 0.1-8.5% for Clotrimazole.
Monitoring of 60 pasteurized milk in different seasons for Clotrimazole and Ketoconazole residue with 0.01 ppm detection limit, demonstrated there is no detectable amounts of these antifungal residues in pasteurized milk.
کلیدواژهها English
