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Omega 3 fatty acids are large group of polyunsaturated fatty acids with long carbon chain length. These fatty acids don’t produce in human body and should be provide by nutrients. The most important omega3 fatty acids are Eicosapentaenoic acid and docosahexaenoic acid that have beneficial effects on the human health. DHA is important in treatment of disease such as cancer, Alzheimer, heart attacks, arteritis romatoid, anxiety, inflammation and malignant diseases. In this research, the aims were to find suitable carbon and nitrogen sources to increase production of omega 3 fatty acid by native strain of schizochytrium. Also quantitative and qualitative effects of different carbon and nitrogen sources were determined on growth of strain production of omega 3 fatty acids rich in DHA. The results showed that the best carbon sources are glucose and glycerol and the best nitrogen source is yeast extract. Glucose increased production of biomass and glycerol increased production of omega 3 fatty acids. Finally, biomass, oil and DHA were produced 0.76, 0.5 and 0.48 g/L by schizochytrium strain in selective medium, respectively. The results showed novel native strain of Schizochytrium has potential for production of omega 3 fatty acids.

Keywords: Omega 3 fatty acids, Docosahexaenoic acid, Native strain, Schizochytrium

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