مجله علوم و صنایع غذایی ایران

مجله علوم و صنایع غذایی ایران

مطالعه نانوالیاف کامپوزیتی الکتروریسی‌شده بر پایه موسیلاژ دانه به (Quince seed) جهت ریزپوشانی ویتامین D3 و باکتری باسیلوس کواگولانس

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه علوم و صنایع غذایی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران
2 دانشجوی دکتری گروه علوم و صنایع غذایی، واحد شهرضا، دانشگاه آزاد اسلامی، شهرضا، ایران
3 دانشجوی گروه علوم و صنایع غذایی، واحد سروستان، دانشگاه آزاد اسلامی، سروستان، ایران
4 دانش‌آموخته کارشناسی ارشد گروه علوم و صنایع غذایی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران
چکیده
در این پژوهش هدف توسعه و بهینه‌سازی نانوالیاف کامپوزیتی الکتروریسی‌شده بر پایه موسیلاژ دانه به، پلی‌وینیل‌الکل (PVA) و کنسانتره پروتئین سبوس برنج (RPC) جهت ریزپوشانی همزمان ویتامین D3 و باکتری پروبیوتیک Bacillus coagulans بود. پس از بررسی نسبت‌های مختلف اجزا و شرایط فرآیند، فرمولاسیون حاوی ۴٪ موسیلاژ، ۴٪ PVA و ۴٪ RPC به‌عنوان ترکیب بهینه انتخاب شد که منجر به تولید الیاف یکنواخت، بدون گره و با قطر میانگین ۳۱۰ ± ۱۸ نانومتر گردید. نتایج آزمون‌های FTIR وجود پیوندهای هیدروژنی و برهم‌کنش‌های بین‌مولکولی مؤثر بین پلیمرها را تأیید کرد. آزمون‌های SEM نیز نشان دادند که ویتامین D3 و B. coagulans به‌صورت یکنواخت در ساختار نانوالیاف توزیع شده‌اند. بررسی کارایی ریزپوشانی نشان داد که بازده انکپسولاسیون ویتامین D3 و B. coagulans به‌ترتیب ۸۷٫۶٪ و ۹۲٫۳٪ بود. آزمون رهایش در محیط شبیه‌سازی‌شده معده (2/1 pH) نشان داد که سیستم نانوالیافی توانست رهایش تدریجی و کنترل‌شده ترکیبات زیست‌فعال را در مدت ۱۸۰ دقیقه فراهم کند، در حالی‌که از تخریب ویتامین و کاهش زنده‌مانی باکتری در شرایط اسیدی جلوگیری شد.نتایج آزمون پایداری طی ۲۰ روز نگهداری نشان داد که نانوالیاف تولیدشده تا بیش از ۸۰٪ از ویتامین D₃ و ۹۰٪ از سلول‌های زنده B. coagulans را حفظ کردند، در حالی‌که در نمونه‌های آزاد کاهش معنی‌داری مشاهده گردید (در سطح 5 درصد). نانوکامپوزیت‌های الکتروریسی‌شده حاصل، با ساختار پایدار، زیست‌سازگار و عملکرد حفاظتی بالا، می‌توانند به‌عنوان حامل‌های نوین و مؤثر برای ریزپوشانی ترکیبات زیست‌فعال در صنایع غذایی و دارویی مورد استفاده قرار گیرند.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Study of Electrospun Composite Nanofibers Based on Quince Seed Mucilage for Microencapsulation of Vitamin D3 and Bacillus coagulans Bacteria

نویسندگان English

sedigheh Yazdanpanah 1
maryam haghighi 2
zahra fahim 3
mitra samimi 4
1 Department of Food Science and Technology, Kaz.C., Islamic Azad University, Kazerun, Iran
2 PhD student in the Department of Food Sciences and Technology , Shah.C., Islamic Azad University, Shahreza, Iran
3 Master's student in the Department of Food Sciences and Technology, Sarv.C., Islamic Azad University, Sarvestan, Iran
4 Master's degree student in the Department of Food Sciences and Technology , Kaz.C., Islamic Azad University, Kazerun, Iran
چکیده English

In this research, the goal of development and optimize electrospun composite nanofibers based on quince seed mucilage, polyvinyl alcohol (PVA), and rice bran protein concentrate (RPC) for the simultaneous microencapsulation of vitamin D₃ and the probiotic bacterium Bacillus coagulans. After investigating different component ratios and processing conditions, a formulation containing 4% mucilage, 4% PVA, and 4% RPC was selected as the optimal composition, resulting in uniform, bead-free nanofibers with an average diameter of 310 ± 18 nm. FTIR analysis confirmed the presence of hydrogen bonding and effective intermolecular interactions among the polymers. SEM observations indicated that vitamin D₃ and B. coagulans were uniformly distributed within the nanofibrous structure. Microencapsulation efficiency results showed encapsulation yields of 87.6% for vitamin D₃ and 92.3% for B. coagulans. Release studies under simulated gastric conditions (pH 2.1) demonstrated that the nanofibrous system provided a gradual and controlled release of the bioactive compounds over 180 minutes, while effectively preventing vitamin degradation and reducing bacterial viability loss under acidic conditions. Stability tests conducted over 20 days the produced nanofibers retained more than 80% of vitamin D₃ and 90% of viable B. coagulans cells, whereas a significant reduction was observed in the free forms (p < 0.05). Overall, the developed electrospun nanocomposites, with their stable structure, biocompatibility, and high protective performance, show strong potential as novel and efficient carriers for the microencapsulation of bioactive compounds in food and pharmaceutical applications.

کلیدواژه‌ها English

Electrospinning
Nanocomposite
Vitamin D₃
Quince seed mucilage
Bacillus coagulans
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