مجله علوم و صنایع غذایی ایران

مجله علوم و صنایع غذایی ایران

استخراج سلولز از پوست ذرت با استفاده از حلال‌های سبز، ارزیابی ویژگی‌ها و خلوص سلولزحاصل

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه علوم و مهندسی صنایع غذایی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان، اصفهان
2 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان، اصفهان ایران
10.48311/fsct.2026.119780.83104
چکیده
فرآیند استخراج زیست ترکیبات می‌تواند در تکمیل زنجیره ارزش محصولات کشاورزی با اهمیت باشد. یکی از روش‌های سازگار با محیط زیست برای استخراج ترکیبات مفید، استفاده از حلال‌های یوتکتیک عمیق (DESs) است. پژوهش حاضر با هدف استخراج سلولز از برگ ذرت به کمک حلال‌ یوتکتیک عمیق انجام شد که در آن، کولین کلراید (ChCl) به عنوان پذیرنده پیوند هیدروژنی و گلیسرول (Gly) به عنوان دهنده پیوند هیدروژنی مورد استفاده قرار گرفت. در این پژوهش، ابتدا ویژگی‌های فیزیکوشیمیایی حلال‌ها، شامل pH و گرانروی، با نسبت‌های مولی متفاوت 1به1، 1به2 و 1به3 از ChClبهGly بررسی شد. سپس برای تعیین مناسب‌ترین نسبت حلال‌ها طی استخراج سلولز، فرآیند استخراج با سه نسبت متفاوت ChCl:Gly انجام و شاخص‌هایی همچون بازده استخراج، میزان هولوسلولز، سلولز، لیگنین و همی‌سلولز ارزیابی گردید. مشخص شد که با تغییر نسبت کولین کلراید به گلیسرول در حلال یوتکتیک، اختلاف معناداری در pH نمونه‌ها وجود ندارد. کمترین ویسکوزیته مربوط به نمونه با نسبت حد وسط ChCl:Gly بود. مشخص شد که با افزایش نسبت مولی گلیسرول از ۱ به ۳، شاخص رفتار جریان از 36/0 به71/0 افزایش می‌یابد. همچنین نتایج نشان داد که در نسبت‌های ۱به۱، 1به2 و ۱به۳، بازده استخراج به ترتیب 28/0±2/69، 32/0±97/69 و 17/0±88/70 درصد و محتوای سلولز 19/0±63/44، 14/0±0/46 و 16/0±0/48 درصد می‌باشد. همچنین میزان ناخالصی لیگنین در سلولز استخراج شده بوسیله حلال دارای نسبت ۱به۳ کولین کلراید به گلیسرول، به‌طور معناداری کمتر از دو نسبت دیگر بود که نشان‌دهنده کارایی بالاتر حلال دارای این نسبت از ChCl:Gly در خالص‌سازی سلولز است.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Extraction of Cellulose from Corn Leaves Using Green Solvents, Evaluation of the Properties and Purity of the Obtained Cellulose

نویسندگان English

Pouya Rezvani 1
Ali Forouhar 2
Hajar Shekarchizadeh 1
1 Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan
2 Department of Food Science and Technology,college of agriculture, Isfahan University of Technology, Isfahan
چکیده English

Extracting bio-components from this agricultural waste, plays a crucial role in completing the agricultural value chain. Among environmentally friendly extraction methods, Deep Eutectic Solvents (DESs), as green solvent, have emerged as a promising approach. This study aimed to extract cellulose from corn leaves using DESs formulated with choline chloride (ChCl) and glycerol (Gly). First, the physicochemical properties of the prepared solvents, including pH and viscosity, were investigated at different ChCl:Gly ratios (1:1, 1:2, and 1:3). Then, cellulose extraction was performed using the three different DES ratios, and key parameters including extraction yield, cellulose purity, holocellulose, lignin, and hemicellulose content were evaluated. The lowest viscosity was observed in the DES with the intermediate ratio (1:2). Furthermore, the Power Law model fitting of viscosity data revealed that with increasing the molar ratio of glycerol from 1 to 3, the flow behavior index increased from 0.36 to 0.71, indicating the modulation of pseudo-plastic behavior and the shift toward Newtonian behavior. The extraction yields at ratios of 1:1, 1:2, and 1:3 were 69.20 ± 0.28%, 69.97 ± 0.32%, and 70.88 ± 0.17%, respectively. The corresponding cellulose contents were 44.63 ± 0.19%, 46.30 ± 0.14%, and 48.28 ± 0.16%, respectively. Notably, the 1:3 ratio demonstrated the highest purification efficiency, significantly reducing residual lignin content. Overall, all the ratios used for the deep eutectic solvent composed of choline chloride and glycerol demonstrated lignin removal and cellulose extraction capability, indicating their effectiveness in cellulose extraction and their potential as a green replacement for conventional solvents.

کلیدواژه‌ها English

Green Solvents
Deep Eutectic Solvent
Cellulose Extraction
Corn leaves
Agricultural Waste
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