مجله علوم و صنایع غذایی ایران

مجله علوم و صنایع غذایی ایران

Using helpful microbial to increase the nutritional value of protein rich foods by provision of beneficial organic compounds

نوع مقاله : مقاله پژوهشی

نویسندگان
1 Department of Horticulture Sciences, Agricultural College, University of Al-Qadisiyah, Iraq
2 Department of Plant Protection, Agricultural College, University of Al-Qadisiyah, Iraq
3 Department of Soil Sciences and Water Resources, Agricultural College, University of Al-Qadisiyah, Iraq
10.48311/fsct.2026.117718.82953
چکیده
Retunhu et al. (2017) assessed the ability of probiotic bacteria from indigenous food sources to produce acetic acid and the possible use thereof as a natural preservative for sheep meat. Pediococcus and Bifidobacterium spp. were isolated from raw milk, shinina and pickle and screened for ability to produce acid in environmentally rich nutritious media consisting of fruit juice and honey. High-performance liquid chromatography and Fourier-transform infrared spectrometry confirmed the presence of the acid after biosynthesis. Bifidobacterium spp. produced more acetic acid than Pediococcus spp., 52.08 mg/L in fruit juice medium being recorded: 12.82 mg/L. The application of the 0.6% purified acetic acid on sheep meat resulted in improved oxidative stability and microbial quality for 18 weeks storage at −10 °C. Lipid oxidation was significantly inhibited through reduction of thiobarbituric acid (TBA) values (0.13 mg malonaldehyde/kg) and peroxide values (PV) at the end of storage recorded as 1.96 mEq/kg. Microbiological data showed a 0 per cent. incidence of the pathogen Clostridium perfringens in treated while psychrotrophic Flavobacterium spp. count was limited to 2,750 cells/g. Sensory evaluation of the treated meat by an expert panel yielded a high acceptability score of 93, compared to 79 for the control. This work indicates that the right “bacteria acetic acid” can provide us with a two-edged gladiatorial “sword” for a reasonable “shortening of the sheep-meat shelf,” next to the crisps at the refrigerator door when q uality, safety and acceptability are helped- “ be it for the health of the public”.
کلیدواژه‌ها
موضوعات

[1]    Fugaban, J. I. I., Vazquez Bucheli, J. E., Park, Y. J., Suh, D. H., Jung, E. S., Franco, B. D. G. D. M., ... & Todorov, S. D. (2022). Antimicrobial properties of Pediococcus acidilactici and Pediococcus pentosaceus isolated from silage. Journal of applied microbiology, 132(1), 311-330. doi: 10.1111/JAM.15205
[2]    Xu, W. M., Liu, Q., Fan, S. Y., Wang, Z. X., Lu, S. R., Liu, J., Piao, H., Ji, W., & Dong, W. 2024. Effect of ginsenoside fermented by Pediococcus acidilactici XM-06 on preventing diarrhea in mice via regulating intestinal barrier function and gut microbiota. Journal of Functional Foods, 123, 106594. https://doi.org/10.1016/j.jff.2024.106594 
[3] Tomičić, Z., Šarić, L., & Tomičić, R. (2025). Potential Future Applications of Postbiotics in the Context of Ensuring Food Safety and Human Health Improvement. Antibiotics, 14(7), 674. https://doi.org/10.3390/antibiotics14070674
[4]    MORAČANIN, Slavica Vesković, et al. Probiotics, Prebiotics and Synbiotics for Combating Antimicrobial Resistance in the Food Chain. Processes, 2025, 13.11: 3483.  https://doi.org/10.3390/pr13113483
[5]    Arslan, N. P., & Aydogan, M. N. (2021). Evaluation of sheep wool protein hydrolysate and molasses as low-cost fermentation substrates for hyaluronic acid production by Streptococcus zooepidemicus ATCC 35246. Waste and Biomass Valorization, 12(2), 925-935. https://doi.org/10.1007/s12649-020-01062-w
[6]    Ahmada Kh, A., AA Abdo, A., Khan, S., Aleryani, H., Mi, S., & Wang, X. (2025). Advancing pickling techniques to enhance bioactive compounds and probiotic content in pickled vegetables. Food Reviews International, 1-27. https://doi.org/10.1080/87559129.2025.2473009
[7]    Kim, J. U., Shahbaz, H. M., Cho, J., Lee, H., & Park, J. (2021). Inactivation of Bacillus cereus spores using a combined treatment of UV-TiO2 photocatalysis and high hydrostatic pressure. Innovative Food Science and Emerging Technologies, 70, 102676. https://doi.org/10.1016/j.ifseet.2021.102676
[8]    Nguyen, V. T., Kwon, Y. M., Park, A. R., Yu, N. H., Choi, G., & Kim, J. C. (2024). Exploring Pediococcus sp. M21F004 for biocontrol of bacterial and fungal phytopathogens. Marine Drugs, 22(12), 534. https://doi.org/10.3390/md22120534
[9]Rahouma, K. (2025). Estimating the Weight Percentage of Acetic Acid in Samples of Commercial Vinegar from Local Markets in Tripoli. AlQalam Journal of Medical and Applied Sciences, 576-580. https://doi.org/10.54361/ajmas-258207   
[10]   Taimooz, S. H., Kareem, B. M., Al-Khaikany, S. A. M., & Shadood, S. N. (2021). Study on the possibility of converting rice husks to organic fertilizer using bacteria Pseudomonas aeregenosa and Celullomonas flavigena. IOP Conference Series: Earth and Environmental Science (Vol. 735, No. 1, p. 012014). IOP Publishing. https://doi.org/10.1088/1755-1315/735/1/012014
[11] Kareem, B. M., Shadood, S. N., & Kadhim, W. S. (2025, February). Safe Hyaluronic Acid Production by Low Cost Agricultural Wastes and Using in Prolonging the Shelf Life of Beef. In IOP Conference Series: Earth and Environmental Science (Vol. 1449, No. 1, p. 012151). IOP Publishing. https://doi.org/10.1088/1755-1315/1449/1/012151
[12] GenStat. General Statistical GenStat Guides Release.12.1. (2009). https://doi.org/10.1111/jam.15205 
[13]  Kerr, M. K., & Churchill, G. A. (2001). Statistical design and the analysis of gene expression microarray data. Genetics Research,77(2),123-128. https://doi.org/10.1017/S0016672301005055
[14]  Mohammed AA.(2022). Extracting hyaluronic acid produced from Streptococcus thermophiles bacteria and studying its effectiveness as an antioxidant of sunflower oil and its effect on some physiological parameters [PhD thesis]. University of Basra, Ministry of Higher Education and Scientific Research, Iraq; 2022.
[15] Kokkinaki, F., & Ordoudi, S. A. (2023). Insights into the FTIR (spectral fingerprint of saffron (Crocus sativus L.) stigmas after gentle drying treatments. Food and Bioprocess Technology, 16(12), 3057-3072. https://doi.org/10.1007/s11947-023-03119-9 
[16]  Yang, Y., Xu, Y., He, X., Guo, M., Chen, J., Luo, L.,&Xiang, J.(2025). Characterizations of lactic acid bacteria derived from pickles and the effects of fermentation on phenolic compounds in peony flowers. Food Chemistry: X, 27, 102430. https://doi.org/10.1016/j.fochx.2025.102430 
[17]   Ozcan, A., Erdal, N. N., & Turhan, I. (2025). Microbial Production of Hyaluronan. In Microbial Production of Food Bioactive Compounds (pp. 1-29). Cham: Springer Nature Switzerland. https://doi.org/10.1007/978-3-030-81403-8_41-1 
[18]  Saleh, H., Mirakzehi, M. T., Bidokhti, H. M., & Kazemi, M. (2025). Evaluation of the effect of bacteriophages and organic acids as a feed additive to reduce Salmonella enteritidis in challenged chickens. Journal of Animal Physiology and Animal Nutrition, 109(3), 881-890. https://doi.org/10.1111/jpn.14103 
[19] Zolfaghari, S. I., & Amin, M. (2025). Identification, molecular diversity, phylogenetic analysis, and antimicrobial activity of lactobacilli isolated from buffalo raw milk. International Dairy Journal, 106278. https://doi.org/10.1016/j.idairyj.2025.106278    ‏‏
[20]  Huang, Y. P., Shi, J. Y., Luo, X. T., Luo, S. C., Cheung, P. C., Corke, H., ... & Zhang, B. B. (2025). How do probiotics alleviate constipation? A narrative review of mechanisms. Critical Reviews in Biotechnology, 45(1), 80-96. https://doi.org/10.1080/07388551.2024.2336531
[21]Tafrishi, R., Ahanchian, H., ali Jafari, S., Pahlevanloo, A., Kianifar, H., Kiani, M., ... & Sly, P. D. (2025). Development and clinical assessment of a novel probiotic candy in the prevention of respiratory infections in asthmatic children. World Allergy Organization Journal, 18(2), 101023. https://doi.org/10.1016/j.waojou.2024.101023  
[22] Harth, M. L., Furlan, F. F., & Horta, A. C. L. (2024). Microbial hyaluronic acid production in the 21 century: a roadmap toward high production, tailored molecular weight. Observatório de la Economía Latinoamericana, 22(3), e3913-e3913. https://doi.org/10.55905/oelv22n3-185
[23]Sabow AB. (2024). Effects of various thawing methods on physicochemical and structural properties of beef. Anbar Journal of Agricultural Sciences. 2024;22(1):301-317. https://doi.org/10.32649/ajas.2024.149228.1237
[24] Yılmaz, B., Mortaş, H., Varlı, S. N., & Ağagündüz, D. (2024). Metabolic engineering of lactic acid bacteria and yeasts for the production of compounds with industrial applications. In Sourdough Microbiota and Starter Cultures for Industry (pp. 223-256). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-031-48604-38 
[25] Bettini, S., Perini, F., Colombi, D., Ghilardi, M., Trabalza-Marinucci, M., & Lasagna, E. (2025). Assessing the impact of biotics on the ruminal microbiome to enhance sustainability, welfare, and performance in beef cattle: highlighting the omics approach. Italian Journal of Animal Science, 24(1), 660-676. https://doi.org/10.1080/1828051X.2025.2465703
[26]  Kaktcham, P. M., Kujawska, M., Kouam, E. M. F., Piame, L. T., Tientcheu, M. L. T., Mueller, J., Felsl, A., Truppel, B., Ngoufack, F. Z., and Hall, L. J. (2025).  Genomic insights into the beneficial potential of Bifidobacterium and Enterococcus strains isolated from Cameroonian infants. Microbial Genomics11(2), 001354. https://doi.org/10.1099/mgen.0.001354 
[27]Chen, J., Chen, X., & Ho, C. L. (2021). Recent development of probiotic bifidobacteria for treating human diseases. Frontiers in bioengineering and biotechnology, 9, 770248. https://doi.org/10.3389/fbioe.2021.770248
[28] Chukwudi, P., Umeugokwe, P. I., Ikeh, N. E., & Amaefule, B. C. (2025). The effects of organic acids on broiler chicken nutrition: A review. Animal Research and One Health, 3(1), 43-53.https://doi.org/10.1002/aro2.85
[29]  Al-Silmawy, N. A., Abd, E. H., Shahad, R. F., & Mohammed, R. J. (2025). Effect of using Pseudomonas fluorescens bacteria, Glomus mosseae fungus and liquid organic fertilizer on soil available nitrogen and phosphorus and some characteristics of fenugreek (Trigonella foenum graecum L.) and choline seed content. Agronomy Research, 23(1), 266-279. https://doi.org/10.15159/ar.25.012
[30]  Al-Ameri, D. T., Al-Baldawy, M. S. M., Hasan, S. T., & Alhasan, A. S. (2020). Inoculation role of Penicillium pinophilum to diverse seed sizes of broad beans (vicia faba). Plant Archives Vol. 20, Supplement 2, 2020 pp. 2101-2104, e-ISSN:2581-6063(online), ISSN:0972-5210.