Journal of food science and technology(Iran)

Journal of food science and technology(Iran)

Evaluation of the effect of oat extract and microbial transglutaminase enzymatic treatment on phenolic compounds, sensory properties and viability of probiotic bacteria in synbiotic yogurt

Document Type : Original Article

Authors
1 Department of Food Science and Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2 Faculty member
10.48311/fsct.2026.117774.82941
Abstract
Functional foods provide important physiological functions by providing bioactive compounds (such as probiotics, prebiotics, and antioxidants) that can contribute to the prevention of common diseases. Oat extract (OE) contains bioactive compounds (fiber beta-glucan, phenols, and tocotrienols) and prebiotics that stimulate the growth of beneficial intestinal bacteria and enhance anti-inflammatory and metabolic properties. However, its addition to yogurt may reduce sensory quality. In the meantime, microbial transglutaminase (MTg) enzymatic treatment is one of the proposed ways to improve the sensory properties of the product by modifying the texture and consistency of yogurt. Therefore, in this study, the effect of enzymatic treatment (0 and 0.02%) of MTg on some properties of synbiotic yogurt containing different concentrations of 0, 10, 20 and 30% OE was investigated during a 21-day storage period. The results showed that addition of OE improved total phenolic content (TPC), probiotic bacteria count and texture score of yogurts, while reducing the color and flavor scores of the samples. TG treatment also increased TPC content and product color and texture scores and reduced the number of probiotic bacteria. With the passage of storage time, although the TPC content increased, the number of probiotics and the sensory properties score of yogurts decreased. However, number of probiotic bacteria was determined to be more than the standard value (7 log CFU/g) after 21 days of storage. Based on the results of this study, the synbiotic yogurt sample treated with 0.02% TG enzyme and containing 20% barley extract was identified as the best yogurt sample.
Keywords
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[1]     Lourens-Hattingh, A., and Viljoen, B. C. 2001. Yogurt as probiotic carrier food. International Dairy Journal, 11(1–2): 1-17.
[2]    Jaman, S., Islam M. Z., Sojib, M. S. I., Hasan, M. S., Khandakar, M. M. H., Bari, M. S. et al. 2022. Physicochemical characteristics, sensory profile, probiotic, and starter culture viability of synbiotic yogurt. Journal of Advanced Veterinary and Animal Research, 9(4): 694-701.
[3]    Hill, C., Guarner, F., Reid, G., Gibson, G. R., Merenstein, D., Pot, B. et al. 2014. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology and Hepatology, 11: 506–514.
[4]    Jooyandeh, H., and Alizadeh Behbahani, B. 2024. Development of a probiotic low-fat set yogurt containing concentrated sweet pepper extract. Food Science & Nutrition, 12(7): 4656-4666.
[5]    Lomer, M. C., Parkes, G. C., and Sanderson, J. D. 2008. Review article: lactose intolerance in clinical practice--myths and realities. Alimentary Pharmacology and Therapeutics, 27(2): 93-103.
[6]    Palomar, M. M., Galdeano, C. M., and Perdigón, G. 2014. Influence of a probiotic lactobacillus strain on the intestinal ecosystem in a stress model mouse. Brain, Behavior, and Immunity, 35: 77-85.
[7]    Ribeiro, M. C. E., Chaves, K. S., Gebara, C., Infante, F. N. S., Grosso, C. R. F., and Gigante, M. L. 2014. Effect of microencapsulation of Lactobacillus acidophilus LA-5 on physicochemical, sensory and microbiological characteristics of stirred probiotic yoghurt. Food Research International, 66: 424-431.
[8]    Mosallaie, F., Jooyandeh, H., Hojjati, M. and Fazlara, A. 2019. Biological reduction of aflatoxin B1 in yogurt by probiotic strains of Lactobacillus acidophilus and Lactobacillus rhamnosus. Food Science and Biotechnology, 29(6): 793-803. 
[9]    Li, H., Zhang, T., Li, Ch., Zheng, Sh., Li, H., and Yu, J. 2020. Development of a microencapsulated synbiotic product and its application in yoghurt. LWT -Food Science and Technology, 122: 109033.
[10] Krasaekoopt, W., Bhandari, B., and Deeth, H. 2003. Evaluation of encapsulation techniques of probiotics for yoghurt. International Dairy Journal, 13(1): 3-13.
[11] Liu, Z., Jiang, Zh., Zhou, K., Li, P., Liu, G., and Zhang, B. 2007. Screening of bifidobacteria with acquired tolerance to human gastrointestinal tract. Anaerobe, 13(5–6): 215-219.
[12] Kailasapathy, K. 2006. Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. LWT -Food Science and Technology, 39(10): 1221-1227.
[13] Oliveira, R. P. S., Perego, P., Oliveira, M. N., and Converti, A. 2011. Effect of inulin as prebiotic and synbiotic interactions between probiotics to improve fermented milk firmness. Journal of Food Engineering, 107(1): 36-40.
[14] Sodini, I., Mattas, J., Phillip, S., and Tong, Ph. S. 2006. Influence of pH and heat treatment of whey on the functional properties of whey protein concentrates in yoghurt. International Dairy Journal, 16(12): 1464-1469.
[15] Roberfroid, M. 2007. Prebiotics: The Concept Revisited. The Journal of Nutrition, 137(3): 830S-837S.
[16] Newburg, D. S. 2000. Oligosaccharides in human milk and bacterial colonization. Journal of Pediatric Gastroenterology and Nutrition, 30: S8–S17.
[17] Slavin, J. 2013. Fiber and prebiotics: Mechanisms and health benefits. Nutrients, 5(4): 1417–1435.
[18] Krasaekoopt, W., and Watcharapoka, S. 2014. Effect of addition of inulin and galactooligosaccharide on the survival of microencapsulated probiotics in alginate beads coated with chitosan in simulated digestive system, yogurt and fruit juice. LWT -Food Science and Technology, 57(2): 761-766.
[19] Swanson, K.S., Gibson, G. R., Hutkins, R. et al. 2020. The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics. Nature Reviews Gastroenterology and Hepatology, 17: 687–701.
[20] Daou, C., and Zhang, H. 2012. Oat beta-glucan: its role in health promotion and prevention of diseases. Comprehensive Reviews in Food Science and Food Safety, 11: 355–365.
[21] Roberfroid, M. B., Gibson, G. R., Hoyles, L., McCartney, A. L., Rastall, R. A., Rowland et al. 2010. Prebiotic effects: metabolic and health benefits. British Journal of Nutrition, 104(S2): S1–S63.
[22] Vulevic, J., Drakoularakou, A., Yaqoob, P., Tzortzis, G., and Gibson, G. R. 2013. Modulation of the fecal microbiota profile and immune function by a novel trans-galactooligosaccharide mixture (B-GOS) in healthy elderly volunteers. The American Journal of Clinical Nutrition, 88(5): 1438–1446.
[23] Wood, P. J. 2010. Cereal β-glucans in diet and health. Journal of Cereal Science, 43(3): 230–236.
[24] Gharibzahedi, S. M. T., Koubaa, M., Barba, F. J., Greiner, R., George, S., and Roohinejad, Sh. 2018. Recent advances in the application of microbial transglutaminase crosslinking in cheese and ice cream products: A review, International Journal of Biological Macromolecules, 107: Part B, 2364-2374.
[25] Torabi, F., Jooyandeh, H., Noshad, M., and Barzegar, H. 2020. Texture, color and total acceptance of synbiotic ultrafiltrated white cheese treated with microbial transglutaminase enzyme during storage period. Journal Food Science & Technology (Iran). 17(98): 135-145. (In Persian)
[26] Kouravand, F., Jooyandeh, H., Barzegar, H., and Hojjati M. 2020. Mechanical, barrier and structural properties of whey protein isolate‐based films treated by microbial transglutaminase. Journal of Microbiology, Biotechnology and Food Sciences, 9(5): 960-964.
[27] Jooyandeh, H., Safari Samani, E., Alizadeh Behbahani, B., and Noshad, M. 2022. Effect of transglutaminase and buffalo milk incorporation on textural parameters and starter cultures viability of strained yogurt. Journal of Food and Bioprocess Engineering, 5(2): 195-202.
[28] Yademellat, M., Jooyandeh, H., and Hojjati, M. 2018. Comparison of some physiochemical and sensory properties of low-fat stirred yogurt containing Persian and Balangu-Shirazi gums. Journal Food Science and Technology (Iran). 14(72): 313-326. (In Persian)
[29] Marinova, D., Ribarov, F., and Atanassova, M. 2005. Total phenolics and total flavonoids in
Bolgarian fruits and vegetables. Journal of the University of Chemical Technology and
Metallurgy, 40(3): 255-260.
[30] Jooyandeh, H., Alizadeh Behbahani, B., and Mehrnia, M. A. 2024. Study on the amounts of Total Phenol, Vitamin C and Antioxidant Properties of Functional Yogurt Containing Bell Pepper Extract during Storage Period. Food Processing and Preservation Journal, 15(4): 1-20.
[31] Torabi, F., Jooyandeh, H., and Noshad, M. 2021. Evaluation of physicochemical, rheological, microstructural, and microbial characteristics of synbiotic ultrafiltrated white cheese treated with transglutaminase. Journal of Food Processing and Preservation, 45(6): e15572.
[32] Bedani, R., Silva Vieira, A. D. and Rossi, E. A. 2014. Tropical fruit pulps decreased probiotic survival to in vitro gastrointestinal stress in synbiotic soy yoghurt with okara during storage. Food Science and Technology. 55: 436-443.
[33] Alqahtani, N. K., Darwish, A. A., El-Menawy, R. K., Alnemr, T. M., and Aly, E. 2021. Textural and organoleptic attributes and antioxidant activity of goat milk yoghurt with added oat flour. International Journal of Food Properties, 24(1): 433–445.
[34] Shahidi, F., and Dissanayaka, C. S. 2023. Phenolic-protein interactions: insight from in-silico analyses – a review. Food Production, Processing and Nutrition, 5, article number 2.
[35] Isaschar-Ovdat, S., and Fishman, A. 2018. Crosslinking of food proteins mediated by oxidative enzymes – A review, Trends in Food Science & Technology, 72: 134-143.
[36] Jooyandeh, H., Mehrnia, M. A., Hojjati, M., and Alizadeh Behbahani, B. 2023. Evaluation of effect of ultrasound and transglutaminase enzyme treatments on yield, physicochemical properties and microstructure of soy cheese. Innovative Food Technologies, 10(2): 119-133.
[37] Abdelshafy, A. M., Mustafa, M. A., Hassan, M. A., and Al-Asmari, F. 2024. Probiotic-fermentation of oat: Safety, strategies for improving quality, potential food applications and biological activities. Trends in Food Science and Technology, 104640.
[38] Cichońska, P., and Ziarno, M. 2021. Legumes and legume-based beverages fermented with lactic acid bacteria as a potential carrier of probiotics and prebiotics. Microorganisms, 10(1): 91.
[39] Zhang, Y., Li, Y., Ren, X., Zhang, X., Wu, Z., and Liu, L. 2023. The positive correlation of antioxidant activity and prebiotic effect about oat phenolic compounds. Food Chemistry, 402: 134231.
[40] Mårtensson, O., Öste, R., and Holst, O. 2002. The effect of yoghurt culture on the survival of probiotic bacteria in oat-based, non-dairy products. Food research international, 35(8): 775-784.
[41] You, H. J., Oh, D. K. and Ji, G. E. 2004. Anticancerogenic effect of a novel Chiroinositolcontaining polysaccharide from Bifidobacterium bifidum BNG. Microbiology Letters, 240(2): 131-136.
[42]Faergemand, M., and Qvist, K. B. 1997. Transglutaminase: Effect on rheological properties, microstructure and permeability of set style acid skim milk gels. Food Hydrocolloids, 11: 287–292.
[43] Farnworth, J. P., Lia, J., Hendricks, G. M., and Guo, M. R. 2006. Effects of transglutaminase
treatment on functional properties and probiotic culture survivability of goat milk yogurt.
Small Ruminant Research, 65: 113-121.
[44] Ozrenk, E, 2006. The use of transglutaminase in dairy products. International Journal of Dairy Technology, 59: 1-7.
[45] Bohamid, A., Jooyandeh, H., and Barzegar, H. 2023. The effect of transglutaminase enzyme and carrageenan gum on sensory, textural and microbial properties of low fat ultrafiltrated cheese. Journal of food science and technology (Iran), 20(139): 1-12. (In Persian)
[46] Glibowski, P., and Skrzypczak, K. 2017. Prebiotic and synbiotic foods. In Microbial production of food ingredients and additives (pp. 155-188). Academic Press.
[47] Demi̇r, H., Simsek, M., and Yıldırım, G. 2021. Effect of oat milk pasteurization type on the characteristics of yogurt. LWT -Food Science and Technology, 135: 110271.
[48] Mahmood, W. A., and Sebo, N. H. 2012. Improvement of yogurt properties by microbial transglutaminase. Journal of Agricultural Sciences, 8(3): 333-342.
[49] Aloglua, H. S., and Oner, Z. 2013. The effect of treating goat’s milk with transglutaminase on chemical, structural, and sensory properties of labneh. Small Ruminant Research, 109: 31-37.
[50] Sanli, T., Sezgin, E., Deveci, O., Senel, E., and Benli, M. 2011. Effect of using transglutaminase on physical, Chemical and sensory properties of set-yoghurt. Food Hydrocolloids, 25: 1477-1481.
[51] Jooyandeh, H., Danesh, E., and Goudarzi, M. 2017. Effect of microbial transglutaminase on physical, rheological, textural and sensory properties of light ice cream. Iranian Food Science and Technology Research Journal, 13(4): 469-479. (In Persian)
[52] Gallardo-Escamilla, F. J., Kelly, A. L., and Delahunty, C. M. 2007. Mouthfeel and flavour of fermented whey with added hydrocolloids. International Dairy Journal, 17(4): 308-315.
[53] Karzan, T. M., Nawal, H. S., and Ashna, T. A. 2015. The effect of microbial transglutaminase
enzyme on some physicochemical and sensory properties of goat’s whey cheese.
International Food Research Journal, 23(2): 688-693.