Journal of food science and technology(Iran)

Journal of food science and technology(Iran)

Stable and dynamic rheological characteristics of gluten-free cake batter enriched with whey protein concentrate, soy protein isolate and basil seed gum

Document Type : Original Article

Authors
1 Ph.D. Graduated of Food Science and Technology, Az.C., Islamic Azad University, Azadshahr, Iran.
2 Department of Food Science and Technology, Az.C., Islamic Azad University, Azadshahr, Iran.
3 Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Abstract
In recent years, the demand for gluten-free food products has been growing exponentially. Thus, research on the effects of various compounds for improving the quality of these products seems necessary. The current study aimed to enhance the rheological properties of a gluten-free batter and cake based on rice flour using whey protein concentrate (WPC), soy protein isolate (SPI) at three levels (0, 5, and 10%), and various amounts of basil seed gum (BSG) (0, 0.5, and 1%).The results revealed that the apparent viscosity in all the samples increased with protein and gum, so the highest apparent viscosity (27.9 Pa⋅s) related to the sample containing 10% SPI and 1% BSG. The flow behavior index of each sample was less than one. Hence, shear thinning behavior (pseudoplasticity) was seen in all batter samples. The storage modulus and loss modulus of the cake batter increased as the proportion of WPC, SPI, and BSG increased. The samples' elasticity was confirmed by the loss tangent value of batter samples with varying concentrations of WPC or SPI, with BSG being less than one. These findings showed that samples with 10% WPC, 10% SPI, and 1% BSG had greater stiffness than other samples at a frequency of 1 Hz. Their complex viscosity values were 277.268 and 523.299 Pa⋅s, respectively. In terms of rheological qualities, cake samples with 10% SPI and 1% BSG were recommended as an alternative to regular cakes for those with celiac disease.  
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Subjects


[1] Salehi, F., Improvement of gluten‐free bread and cake properties using natural hydrocolloids: A review. Food science & nutrition, 2019. 7(11): p. 3391-3402.
[2] Seyedahmadi, S., et al., Enhancing the quality of rice-based gluten-free bread using sourdoughs fermented with Lactobacillus fermentum and Lactobacillus plantarum. Scientific Reports, 2025. 15(1): p. 26543.
[3] Fajardo, V., et al., Updated food composition database for cereal-based gluten free products in Spain: is reformulation moving on? Nutrients, 2020. 12(8): p. 2369.
[4] Burbano Moreano, J.J., D.M. Cabezas, and M.J. Correa, Effect of walnut flour addition on rheological, thermal and microstructural properties of a gluten free-batter. 2022.
[5] Rodríguez, R., et al., Effect of microwave technology and upcycled orange fibre on the quality of gluten-free muffins. LWT, 2022. 158: p. 113148.
[6] Gharekhani, M., et al., Sourdoughs fermented by autochthonous Lactobacillus strains improve the quality of gluten‐free bread. Food Science & Nutrition, 2021. 9(11): p. 6372-6381.
[7] Rosell, C.M., M. Aalami, and S.A. Mahdavi, Innovative gluten‐free products. Innovative processing technologies for healthy grains, 2020: p. 177-198.
[8] Mohammadzadeh, F., et al., Optimizing the formulation of gluten-free sponge cake based on rice flour containing tragacanth gum and date pomace powder using mixed statistical design. Journal of food science and technology (Iran), 2025. 22(161): p. 229-243.
[9] Gharekhani, M., et al., Physical and aerodynamic properties of paddy and white rice as a function of moisture content. Quality Assurance and Safety of Crops & Foods, 2013. 5(3): p. 187-197.
[10] Moniri, H., R. Farahmandfar, and A. Motamedzadegan, Investigation of hot air and foam‐mat dried cress seed gum by FT‐IR, zeta potential, steady shear viscosity, dynamic oscillatory behavior, and other physical properties. Food science & nutrition, 2020. 8(4): p. 2143-2155.
[11] Azmoon, E., et al., The effects of hydrocolloids-protein mixture as a fat replacer on physicochemical characteristics of sugar-free muffin cake: Modeling and optimization. Foods, 2021. 10(7): p. 1549.
[12] Rahim Monfared, M., L. Nouri, and A. Mohammadi Nafchi, The effects of sesame protein isolate and transglutaminase enzyme on the quality characteristics of gluten-free batter and cake. Journal of Food Measurement and Characterization, 2023. 17(5): p. 4881-4891.
[13] Yıldız, E., S. Şumnu, and S. Şahin, Effects of buckwheat flour, gums and proteins on rheological properties of gluten-free batters and structure of cakes. Quality Assurance and Safety of Crops & Foods, 2018. 10(3): p. 245-254.
[14] Pu, X., et al., Development and characterization of acid-induced whey protein concentrate and egg white protein composite gel. Lwt, 2022. 164: p. 113624.
[15] Sahagún, M., et al., Influence of protein source on the characteristics of gluten-free layer cakes. Lwt, 2018. 94: p. 50-56.
[16] Ammar, I., et al., Optimization of gluten‐free sponge cake fortified with whey protein concentrate using mixture design methodology. Food Chemistry, 2021. 343: p. 128457.
[17] Nazir, S. and I.A. Wani, Functional characterization of basil (Ocimum basilicum L.) seed mucilage. Bioactive Carbohydrates and Dietary Fibre, 2021. 25: p. 100261.
[18] Pourmohammadi, O., S.H. Hosseini Ghaboos, and S. Jafarian, Physicochemical, rheological, and sensorial properties of bread supplemented with pumpkin powder and basil seed gum. Journal of Food Processing and Preservation, 2020. 44(12): p. e14739.
[19] Razavi, S.M., et al., Optimisation study of gum extraction from Basil seeds (Ocimum basilicum L.). International Journal of Food Science and Technology, 2009. 44(9): p. 1755-1762.
[20] Turabi, E., G. Sumnu, and S. Sahin, Rheological properties and quality of rice cakes formulated with different gums and an emulsifier blend. Food hydrocolloids, 2008. 22(2): p. 305-312.
[21] AACC, Approved methods of the American association of cereal chemists (Vol. 1): American Association of Cereal Chemists. 2000.
[22] Rahmati, F., et al., Investigation of rheological, physicochemical, and sensorial properties of traditional low‐fat Doogh formulated. Food Science & Nutrition, 2023. 11(11): p. 7218-7228.
[23] Arab, K., et al., Gelling and rheological properties of a polysaccharide extracted from Ocimum album L. seed. International Journal of Biological Macromolecules, 2023. 246: p. 125603.
[24] Delcour, J. and R.C. Hoseney, Principles of cereal science and technology authors provide insight into the current state of cereal processing. Cereal Foods World, 2010. 55(1): p. 21-22.
[25] Fathi, B., et al., Utilization of heat‐moisture treated proso millet flour in production of gluten‐free pound cake. Journal of food quality, 2016. 39(6): p. 611-619.
[26] Abbaszadeh, F., et al., Effect of Pickering emulsion stabilized by soy protein nanoparticles on physical and rheological properties of gluten‐free cake batter. Journal of Food Processing and Preservation, 2023. 2023(1): p. 3348944.
[27] Chen, R.H. and W.Y. Chen, Rheological properties of the water-soluble mucilage of a green laver, Monostroma nitidium. Journal of Applied phycology, 2001. 13: p. 481-488.
[28] Ahmadi, F., N. Aghajani, and A. Gohari Ardabili, Response surface optimization of cupcake physicochemical and sensory attributes during storage period: Effect of apricot kernel flour addition. Food Science & Nutrition, 2022. 10(3): p. 661-677.
[29] Lazaridou, A., et al., Effects of hydrocolloids on dough rheology and bread quality parameters in gluten-free formulations. Journal of food engineering, 2007. 79(3): p. 1033-1047.
[30] Dogan, S.F., S. Sahin, and G. Sumnu, Effect of soy and rice flour addition on batter rheology and quality of deep-fat fried chicken nuggets. Journal of Food Engineering, 2005. 71: 127– 132.
[31] Mahmood, K., et al., Wheat flour and gum cordia composite system: Pasting, rheology and texture studies. Food Science and Technology, 2018. 38(4): p. 691-697.
[32] Shaabani, S., et al., The effect of chickpea protein isolate in combination with transglutaminase and xanthan on the physical and rheological characteristics of gluten free muffins and batter based on millet flour. LWT, 2018. 90: p. 362-372.
[33] Noorlaila, A., et al., Effects of xanthan gum and HPMC on physicochemical and microstructure properties of sponge cakes during storage. Journal of food science and technology, 2017. 54: p. 3532-3542.
[34] Gope, S., et al., Effect of alcohol-acid modification on physicochemical, rheological and morphological properties of glutinous rice starch. International journal of biological macromolecules, 2016. 93: p. 860-867.
[35] Waziiroh, E., et al., Ohmic baking of gluten-free bread: role of non-gluten protein on GF bread structure and properties. International Journal of Food Science and Technology, 2023. 58(2): p. 595-609.
[36] Matos, M.E., T. Sanz, and C.M. Rosell, Establishing the function of proteins on the rheological and quality properties of rice based gluten free muffins. Food Hydrocolloids, 2014. 35: p. 150-158.
[37] Villanueva, M., et al., Impact of acidification and protein fortification on rheological and thermal properties of wheat, corn, potato and tapioca starch-based gluten-free bread doughs. Food Science and Technology, 2018. 96: 446-454.
38. Chompoorat, P., et al., Physical and dynamic oscillatory shear properties of gluten-free red kidney bean batter and cupcakes affected by rice flour addition. Foods, 2020. 9(5): p. 616.
[39] Herranz, B., W. Canet, and M. Alvarez, Dolores Corn starch and egg white enriched gluten-free chickpea flour batters: Rheological and structural properties. International Journal of Food Properties, 2017. 20: 489-506.
[40] Patil, S., et al., Pasting, viscoelastic and rheological characterization of gluten free (cereals, legume and underutilized) flours with reference to wheat flour. Journal of Food Science and Technology, 2020. 57: p. 2960-2966.
[41] Benazir, S., et al., Influence of hydrocolloids on rheological properties of gluten-free dough based on corn flour. Journal of Postharvest Technology, 2019. 7(1): p. 72-83.