Journal of food science and technology(Iran)

Journal of food science and technology(Iran)

Combined use of sodium alginate and calcium lactate to extend the shelf life of edible button mushroom.

Document Type : Original Article

Authors
Department of Horticulture, Faculty of Agriculture, Shahed University, Tehran, Iran
Abstract
This study aimed to improve the shelf life and maintain the quality of edible button mushrooms (Agaricus bisporus) through edible coating treatments. A factorial experiment was conducted in a completely randomized design with three replications, involving sodium alginate (0, 0.5, and 1%), calcium lactate (0 and 0.5%), and storage durations (0, 7, 14, and 21 days). After applying the treatments, the mushrooms were packaged in cellophane and stored at 4°C. Microbiological, physicochemical, and sensory parameters were evaluated. The results showed that the combined treatment of 0.5% sodium alginate and 0.5% calcium lactate significantly maintained the quality of the mushrooms, reducing weight loss by 50%, inhibiting browning by 38%, decreasing bacterial growth by 38%, preserving optimal texture firmness, and reducing fluctuations in soluble solids compared to the control. Additionally, this treatment significantly preserved the sensory characteristics of the mushrooms. In contrast, the treatment with 1% sodium alginate and 0.5% calcium lactate, despite increasing calcium content to 0.76 mg/g dry weight, showed weaker performance in controlling browning and maintaining sensory attributes. Single treatments were also effective in some parameters but were less effective than the combined treatment in preventing quality deterioration. In conclusion, the simultaneous use of 0.5% sodium alginate and 0.5% calcium lactate is recommended as an effective method to extend the shelf life and preserve the quality of edible button mushroom.
Keywords

Subjects


 
[1] Rathore, H., Prasad, S., and Sharma, S. (2017). Mushroom nutraceuticals for improved nutrition and better human health: A review. PharmaNutrition, 5(2), 35-46. 
[2] Wu, X., Guan, W., Yan, R., Lei, J., Xu, L., and Wang, Z. (2016). Effects of UV-C on antioxidant activity, total phenolics and main phenolic compounds of the melanin biosynthesis pathway in different tissues of button mushroom. Postharvest Biology and Technology, 118, 51-58. 
[3] Valverde, M. E., Hernández-Pérez, T., and Paredes-López, O. (2015). Edible mushrooms: improving human health and promoting quality life. International Journal of Microbiology, 2015, 1-14. 
[4] Cardwell, G., Bornman, J. F., James, A. P., and Black, L. J. (2018). A review of mushrooms as a potential source of dietary vitamin D. Nutrients, 10(10), 1498.
[5] Brennan, M., Le Port, G., and Gormley, R. (2000). Post-harvest treatment with citric acid or hydrogen peroxide to extend the shelf life of fresh sliced mushrooms. LWT-Food Science and Technology, 33 (4), 285-289. 
[6] Liang, Y., Luo, K., Wang, B., Huang, B., Fei, P., and Zhang, G. (2024). Inhibition of polyphenol oxidase for preventing browning in edible mushrooms: A review. Journal of Food Science, 89(11), 6796-6817.
[7] Lagnika, C., Zhang, M., and Mothibe, K. J. (2013). Effects of ultrasound and high pressure argon on physico-chemical properties of white mushrooms (Agaricus bisporus) during postharvest storage. Postharvest Biology and Technology, 82, 87-94. 
[8] Carrasco, J., and Preston, G. M. (2020). Growing edible mushrooms: A conversation between bacteria and fungi. Environmental Microbiology, 22(3), 858-872. 
[9] Donglu, F., Wenjian, Y., Kimatu, B. M., Xinxin, A., Qiuhui, H., and Liyan, Z. (2016). Effect of nanocomposite packaging on postharvest quality and reactive oxygen species metabolism of mushrooms (Flammulina velutipes). Postharvest Biology and Technology, 119, 49-57. 
[10] Guillaume, C., Schwab, I., Gastaldi, E., and Gontard, N. (2010). Biobased packaging for improving preservation of fresh common mushrooms (Agaricus bisporus L.). Innovative Food Science and Emerging Technologies, 11(4), 690-696.
[11] Han, L., Qin, Y., Liu, D., Chen, H., Li, H., and Yuan, M. (2015). Evaluation of biodegradable film packaging to improve the shelf-life of Boletus edulis wild edible mushrooms. Innovative Food Science and Emerging Technologies, 29, 288-294. 
[12] Kale, S. J., Nath, P., Kannaujia, P., and Dukare, A. (2021). Hydrogen peroxide washing induced changes in postharvest quality of button mushrooms (Agaricus bisporus) during storage. Journal of Agriculture and Food Research, 1(1), 119-130.
[13] Meng, D., Song, T., Shen, L., Zhang, X., and Sheng, J. (2012). Postharvest application of methyl jasmonate for improving quality retention of Agaricus bisporus fruit bodies. Journal of Agricultural and Food Chemistry, 60(23), 6056-6062. 
[14] Hu, Y. H., Chen, C. M., Xu, L., Cui, Y., Yu, X. Y., Gao, H. J., and Chen, Q. X. (2015). Postharvest application of 4-methoxy cinnamic acid for extending the shelf life of mushroom (Agaricus bisporus). Postharvest Biology and Technology, 104, 33-41.
[15] NeysariFam, V., Golmohammadi, A., Tabatabaekoloor, R., Tahmasebi, M., Nematollahzadeh, A., Razavi, M. S., ... and Ghaani, M. (2025). Preservation of button mushrooms using modified atmosphere and bio-nanocomposite film: A combined approach. Applied Food Research, 101043.
[16] Oz, A. T., Ulukanli, Z., Bozok, F., and Baktemur, G. (2015). The postharvest quality, sensory and shelf life of Agaricus bisporus in active map. Journal of Food Processing and Preservation, 39(1), 100-106.
[17] Dama, C. L., Kumar, S., Mishra, B. K., Shukla, K. B., Mathur, S., and Doshi, A. (2010). Antioxidative enzymatic profile of mushrooms stored at low temperature. Journal of Food Science and Technology, 47, 650-655. 
[18] Li, D., Qin, X., Tian, P., and Wang, J. (2016). Toughening and its association with the postharvest quality of king oyster mushroom (Pleurotus eryngii) stored at low temperature. Food Chemistry, 196, 1092-1100. 
[19] Lin, Q., Lu, Y., Zhang, J., Liu, W., Guan, W., and Wang, Z. (2017). Effects of high CO2 in-package treatment on flavor, quality and antioxidant activity of button mushroom (Agaricus bisporus) during postharvest storage. Postharvest Biology and Technology, 123, 112-118.
[20] Lagnika, C., Zhang, M., Nsor-Atindana, J., and Bashari, M. (2014). Effects of ultrasound and chemical treatments on white mushroom (Agaricus bisporus) prior to modified atmosphere packaging in extending shelf-life. Journal of Food Science and Technology, 51, 3749-3757. 
[21] Mohebbi, M., Ansarifar, E., Hasanpour, N., and Amiryousefi, M. R. (2012). Suitability of aloe vera and gum tragacanth as edible coatings for extending the shelf life of button mushroom. Food and Bioprocess Technology, 5, 3193-3202. 
[22] Sharma, P., Shehin, V. P., Kaur, N., and Vyas, P. (2019). Application of edible coatings on fresh and minimally processed vegetables: A review. International Journal of Vegetable Science, 25(3), 295-314.
[23] Jiang, T. (2013). Effect of alginate coating on physicochemical and sensory qualities of button mushrooms (Agaricus bisporus) under a high oxygen modified atmosphere. Postharvest Biology and Technology, 76, 91-97. 
[24] Aguilar, K. C., Tello, F., Bierhalz, A. C., Romo, M. G. G., Flores, H. E. M., and Grosso, C. R. (2015). Protein adsorption onto alginate-pectin microparticles and films produced by ionic gelation. Journal of Food Engineering, 154, 17-24. 
[25] Sow, L. C., Toh, N. Z. Y., Wong, C. W., and Yang, H. (2019). Combination of sodium alginate with tilapia fish gelatin for improved texture properties and nanostructure modification. Food Hydrocolloids, 94, 459-467.
[26] Sharma, A., and Singh, A. K. (2025). Sodium alginate: A green biopolymer resource-based antimicrobial edible coating to enhance fruit shelf-life: A review. Colloids and Interfaces, 9(3), 32.
[27] Naser, F., Rabiei, V., Razavi, F., and Khademi, O. (2018). Effect of calcium lactate in combination with hot water treatment on the nutritional quality of persimmon fruit during cold storage. Scientia Horticulturae, 233, 114-123. 
[28] Chaudhary, B., Azam, M., Khan, A. S., & Abbas, H. (2025). Effect of pre-harvest calcium chloride applications on physicochemical quality traits and postharvest life of grapes during cold storage. Journal of Food Measurement and Characterization, 19, 9032–9051.
[29] Nasri, E., Khademi, O., Saba, M. K., and Ebrahimi, R. (2022). Extension of button mushroom storability by ultrasound treatment in combination with calcium lactate. Journal of Food Measurement and Characterization, 16(1), 1-9. 
[30] Aguayo, E., Escalona, V. H., and Artés, F. (2008). Effect of hot water treatment and various calcium salts on quality of fresh-cut 'Amarillo' melon. Postharvest Biology and Technology, 47(3), 397-406.
[31] Manganaris, G. A., Vasilakakis, M., Diamantidis, G., and Mignani, I. (2005). Effect of calcium additives on physicochemical aspects of cell wall pectin and sensory attributes of canned peach (Prunus persica (L) Batsch cv Andross). Journal of the Science of Food and Agriculture, 85(10), 1773-1778.
[32] Silveira, A. C., Aguayo, E., Chisari, M., and Artés, F. (2011). Calcium salts and heat treatment for quality retention of fresh-cut 'Galia' melon. Postharvest Biology and Technology, 62(1), 77-84. 
[33] Luna-Guzmán, I., and Barrett, D. M. (2000). Comparison of calcium chloride and calcium lactate effectiveness in maintaining shelf stability and quality of fresh-cut cantaloupes. Postharvest Biology and Technology, 19(1), 61-72. 
[34] Hassani, F., and Khademi, O. (2018). Effect of calcium chloride and salicylic acid treatments on quality characteristics and shelf life of fresh-cut kiwifruit. Journal of Food Measurement and Characterization, 12(1), 658-666. 
[35] Guan, W. (2012). Effects of UV-C treatment on inactivation of Escherichia coli O157: H7, microbial loads, and quality of button mushrooms. Postharvest Biology and Technology, 64(1), 119-125. 
[36] Khovitari, S., and Khademi, O. (2022). Determination the best source of calcium for button mushroom conservation. Journal of Horticulture and Postharvest Research, 5(2), 177-186.
[37] Eroğul, D., Gundogdu, M., Sen, F., and Tas, A. (2024). Impact of postharvest calcium chloride treatments on decay rate and physicochemical quality properties in strawberry fruit. BMC Plant Biology, 24(1), 1088.
[38] Sohail, M., Ayub, M., Khalil, S. A., Zeb, A., Ullah, F., Afridi, S. R., and Ullah, R. (2015). Effect of calcium chloride treatment on postharvest quality of peach fruit during cold storage. International Food Research Journal, 22(6), 2387-2393. 
[39] Kalita, P., Roy, P. K., Bhattacharjee, B., Goswami, A., Pachuau, L., and Roy, S. (2025). Alginate-based coating in post-harvest fruits preservation: A comprehensive review. International Journal of Biological Macromolecules, 145799.
[40] Mirshekari, A., Madani, B., and Golding, J. B. (2019). Aloe vera gel treatment delays postharvest browning of white button mushroom (Agaricus bisporus). Journal of Food Measurement and Characterization, 13(2), 1250-1256. 
[41] Ahmad, F., Muhammad, A., Hashmi, M. S., Ahmad, A., Alam, S., Din, K. U., and Siyab, A. (2024). Pre-storage calcium chloride and aloe vera gel coatings mitigate internal browning and senescence scald in ‘Conference’pears. Scientia Horticulturae, 325, 112684.
[42] Aguayo, E., Requejo-Jackman, C., Stanley, R., and Woolf, A. (2015). Hot water treatment in combination with calcium ascorbate dips increases bioactive compounds and helps to maintain fresh-cut apple quality. Postharvest Biology and Technology, 110, 158-165.