مجله علوم و صنایع غذایی ایران

مجله علوم و صنایع غذایی ایران

Preserving Wheat Seed Quality: Low-Level Ozone Fumigation as a Strategy Against Khapra Beetle Damage

نوع مقاله : مقاله پژوهشی

نویسندگان
Hussein T M Al-Sharees 1
Mohammed Sh. Mansor 2
1 Mesopotamia General Seed Company, Ministry of Agriculture, Baghdad, Iraq
2 Department of Plant Protection, Faculty of Agriculture, University of Tikrit, Tikrit, Iraq
10.48311/fsct.2026.117587.82924
چکیده
A laboratory colony was established from field-collected adults and reared on sterilized wheat at 30 ± 2°C, 65 ± 5% RH, and a 0:24 (L:D) h photoperiod. Synchronized third-instar larvae, fourth-instar larvae, and adults (<24 h old) were exposed to 50 ± 5 ppm ozone gas in a sealed 1000 L fumigation chamber at 38 ± 2°C and 49 ± 5% RH. A factorial completely randomized design (CRD) with life stage and exposure time (0, 6, 12, 18, 24, 48, 72 h) as factors was employed, including a positive control (aluminum phosphide at 3 g/tonne for 72 h). In laboratory bioassays, insects were directly exposed in gas-permeable mesh bags. For simulated storage trials, insect samples were embedded within 10 kg wheat bags inside the fumigation chamber. Mortality was assessed 14 days post-treatment. Results indicated a time-dependent mortality for all stages. Under direct exposure, adults were highly susceptible, reaching 100% mortality within 12 hours. Larval stages were more tolerant, requiring 36 hours to achieve 96.6% mortality. In simulated storage, the efficacy of ozone was attenuated due to reduced penetration, requiring 72 hours of exposure to achieve 100% mortality for both larvae and adults. At 72 hours, ozone efficacy was statistically equivalent to aluminum phosphide for adults and superior for larvae. Seed germination was not adversely affected by any treatment.
کلیدواژه‌ها
Postharvest Technology
Stored Product Protection
Trogoderma granarium
Non-chemical Fumigation
Seed Viability
Sustainable Agriculture

موضوعات

عنوان مقاله English

Preserving Wheat Seed Quality: Low-Level Ozone Fumigation as a Strategy Against Khapra Beetle Damage

نویسندگان English

Hussein T M Al-Sharees 1
Mohammed Sh. Mansor 2
1 Mesopotamia General Seed Company, Ministry of Agriculture, Baghdad, Iraq
2 Department of Plant Protection, Faculty of Agriculture, University of Tikrit, Tikrit, Iraq
چکیده English

A laboratory colony was established from field-collected adults and reared on sterilized wheat at 30 ± 2°C, 65 ± 5% RH, and a 0:24 (L:D) h photoperiod. Synchronized third-instar larvae, fourth-instar larvae, and adults (<24 h old) were exposed to 50 ± 5 ppm ozone gas in a sealed 1000 L fumigation chamber at 38 ± 2°C and 49 ± 5% RH. A factorial completely randomized design (CRD) with life stage and exposure time (0, 6, 12, 18, 24, 48, 72 h) as factors was employed, including a positive control (aluminum phosphide at 3 g/tonne for 72 h). In laboratory bioassays, insects were directly exposed in gas-permeable mesh bags. For simulated storage trials, insect samples were embedded within 10 kg wheat bags inside the fumigation chamber. Mortality was assessed 14 days post-treatment. Results indicated a time-dependent mortality for all stages. Under direct exposure, adults were highly susceptible, reaching 100% mortality within 12 hours. Larval stages were more tolerant, requiring 36 hours to achieve 96.6% mortality. In simulated storage, the efficacy of ozone was attenuated due to reduced penetration, requiring 72 hours of exposure to achieve 100% mortality for both larvae and adults. At 72 hours, ozone efficacy was statistically equivalent to aluminum phosphide for adults and superior for larvae. Seed germination was not adversely affected by any treatment.

کلیدواژه‌ها English

Postharvest Technology
Stored Product Protection
Trogoderma granarium
Non-chemical Fumigation
Seed Viability
Sustainable Agriculture
[1]       Kumar, N., Upadhyay, G., Chhetri, K. B., Harsha, B. R., Malik, G. K., Kumar, R., ... & Gill, S. C. (2023). Pre-and post-harvest management of wheat for improving the productivity, quality, and resource utilization efficiency. In Wheat science (pp. 57-106). CRC Press.‏
[2]       Manandhar, A., Milindi, P., & Shah, A. (2018). An overview of the post-harvest grain storage practices of smallholder farmers in developing countries. Agriculture, 8(4), 57.‏
[3]       Athanassiou, C. G., Phillips, T. W., & Wakil, W. (2019). Biology and control of the khapra beetle, Trogoderma granarium, a major quarantine threat to global food security. Annual Review of Entomology, 64(1), 131-148.‏
[4]       Wu, Y., Domingue, M. J., McGraw, A. R., Vieira, K. A., Palmeri, M. Z., & Myers, S. W. (2023). Development of an array of molecular tools for the identification of khapra beetle (Trogoderma granarium), a destructive beetle of stored food products. Scientific Reports, 13(1), 3327.‏
[5]       Hashem, A. S., Amin, H. E., Ramadan, M. M., Abdel-Hady, A. A., Awadalla, H. S., Al-Solami, H. M., ... & Guedes, R. N. C. (2025). Unraveling grain vulnerability to the khapra beetle (Trogoderma granarium): Structure and mineral content. Journal of Stored Products Research, 112, 102647.‏
[6]       Abbas, T., Zahir, Z. A., Naveed, M., & Kremer, R. J. (2018). Limitations of existing weed control practices necessitate development of alternative techniques based on biological approaches. Advances in Agronomy, 147, 239-280.‏
[7]       Damalas, C. A., & Koutroubas, S. D. (2020). Botanical pesticides for eco‐friendly pest management: Drawbacks and limitations. Pesticides in Crop Production: Physiological and Biochemical Action, 181-193.‏
[8]       Guru, P. N., Mridula, D., Dukare, A. S., Ghodki, B. M., Paschapur, A. U., Samal, I., ... & Subbanna, A. R. (2022). A comprehensive review on advances in storage pest management: Current scenario and future prospects. Frontiers in Sustainable Food Systems, 6, 993341.‏
[9]       Rajendran, S. (2020). Insect pest management in stored products. Outlooks on Pest Management, 31(1), 24-35.‏
[10]    Wang, B., Yun, J., Ye, C., Xu, S., Guo, W., Zhao, F., ... & Bi, Y. (2024). A novel polyethylene nanopackaging combined with ozone fumigation delayed the browning and softening of Agaricus bisporus during postharvest storage. Postharvest Biology and Technology, 210, 112771.‏
[11]    Pandiselvam, R., Subhashini, S., Banuu Priya, E. P., Kothakota, A., Ramesh, S. V., & Shahir, S. (2019). Ozone based food preservation: A promising green technology for enhanced food safety. Ozone: Science & Engineering, 41(1), 17-34.‏
[12]    Osman, K. A., Mohamed, H. H. E., & Salama, M. S. (2024). Ozone treatment as a green technology for removing chlorpyrifos residues from vegetables and its impact on the quality of vegetables. Ozone: Science & Engineering, 46(6), 558-572.‏
[13]    Pawar, E. K. R., Atkari, E. V. T., Pawar, D. A., & Ukey, E. P. D. (2021). Ozone Treatment: The Green Technology in Food Industry. Chapter October.‏
[14]    Al-Hayali, T. S. A., & Qader, F. A. (2024, July). The effect of five wheat varieties on khapra beetle, Trogoderma granarium everts (Coleoptera: Dermestidae) during four storage months. In IOP Conference Series: Earth and Environmental Science (Vol. 1371, No. 3, p. 032023). IOP Publishing.‏
[15]    Krogmann, L., Holstein, J., Eymann, J., Degreef, J., Häuser, C., Monje, J. C., ... & Vanden Spiegel, D. (2010). Preserving and specimen handling: insects and other invertebrates. Manual on field recording techniques and protocols for all taxa biodiversity inventories, 2, 463-481.
[16]    Hoshmand, R. (2018). Design of experiments for agriculture and the natural sciences. Chapman and Hall/CRC.
[17]    Mahmoud, M. A., Abo Laban, G. F., Ibrahim, I. S., El-Dessouki, W. A., Metwaly, K. H., Saba, R. M., & Zahra, A. A. (2023). Influence of ozone gas on the khapra beetle, Trogoderma granarium (Coleoptera: Dermestidae) in stored wheat. Applied Entomology and Zoology, 58(2), 181-191.‏‏
[18]    Dong, X., Agarwal, M., Xiao, Y., Ren, Y., Maker, G., & Yu, X. (2022). Ozone efficiency on two coleopteran insect pests and its effect on quality and germination of barley. Insects, 13(4), 318.‏
[19]    Gad, H. A., Laban, G. F. A., Metwaly, K. H., Al-Anany, F. S., & Abdelgaleil, S. A. (2021). Efficacy of ozone for Callosobruchus maculatus and Callosobruchus chinensis control in cowpea seeds and its impact on seed quality. Journal of Stored Products Research, 92, 101786.‏
[20]    Zahra, A. A., Bedewy, M. M., Elsaffany, A. H., Metwaly, K. H., & Gad, H. A. (2025). Insecticidal effect of ozone on larvae of Tenebrio molitor and their enzyme activity. Journal of Plant Diseases and Protection, 132(4), 124.‏
[21]    Al-Emara, M. S., Alyousuf, A. A., & Abass, M. H. (2021). Efficacy of ozone gas against all stages of red flour beetle, Tribolium castaneum (Herbst)(Coleoptera: Tenebrionidae) at different temperatures and exposure periods. Basrah Journal of Agricultural Sciences, 34(2), 240-252.
[22]    Ghazawy, N. A., Zinhoum, R. A., Ali, M. M., Afify, A., & Hussain, H. B. (2021). Efficacy of ozone on mortality, super oxide dismutases, nitric oxide enzymes and ultrastructural alterations on some stored product insect larvae in Egypt. African Entomology, 29(1), 17-31.
[23]    Bernate, I., Kince, T., Radenkovs, V., Juhnevica-Radenkova, K., Cinkmanis, I., Bruveris, J., & Sabovics, M. (2024). Impact of Ozone Exposure on the Biochemical Composition of Wheat, Broccoli, Alfalfa, and Radish Seeds During Germination. Agronomy, 14(11), 2571.‏
[24]    Sandhu, H. P., Manthey, F. A., & Simsek, S. (2012). Ozone gas affects physical and chemical properties of wheat (Triticum aestivum L.) starch. Carbohydrate Polymers, 87(2), 1261-1268.‏
[25]    Piechowiak, T., Józefczyk, R., & Balawejder, M. (2018). Impact of ozonation process of wheat flour on the activity of selected enzymes. Journal of Cereal Science, 84, 30-37.‏