مجله علوم و صنایع غذایی ایران

مجله علوم و صنایع غذایی ایران

ارزیابی ویژگی‌های تکنولوژیکی سویه Lactobacillus acidophilus TMPC 41127 جداسازی‌شده از پنیر سنتی خیکی

نوع مقاله : پژوهشی اصیل

نویسندگان
1 دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 دانشجوی دکتری، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
3 استادیار گروه ایمنی و کنترل کیفیت مواد غذایی، پژوهشکده علوم و فناوری مواد غذایی، مشهد، ایران.
4 دانشجوی کارشناسی ارشد، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
چکیده
در این مطالعه، ویژگی‌های تکنولوژیکی سویه پروبیوتیک Lactobacillus acidophilus TMPC 41127، جداسازی‌شده از پنیر خیکی، به‌منظور ارزیابی پتانسیل آن برای کاربردهای صنعتی مورد بررسی قرار گرفت. ویژگی‌های مورد نظر شامل مقاومت حرارتی، تولید اگزوپلی‌ساکارید (EPS)، توانایی اسیدی‌سازی، فعالیت پروتئولیتیکی و فعالیت اتولیتیکی بودند.نتایج نشان داد که سویه L. acidophilus TMPC 41127 مقاومت حرارتی قابل قبولی از خود نشان می‌دهد؛ بالاترین نرخ بقا (74/53 %) در دمای ۵۰ درجه سانتی‌گراد ثبت شد. این سویه به عنوان یک اسیدی‌کننده قوی نیز طبقه‌بندی شد، زیرا توانست pH اولیه 49/6 را در مدت ۲۴ ساعت به 85/4 کاهش دهد. علاوه بر این، توانایی قابل‌توجهی در تولید EPS به شکل موکوئید از خود نشان داد. فعالیت پروتئولیتیکی آن با تشکیل هاله‌ای به قطر 03/22 میلی‌متر تأیید شد. در نهایت، فعالیت اتولیتیکی سویه در دمای ۴ درجه سانتی‌گراد در سطح متوسط (21/20%) و در دمای ۲۰- درجه سانتی‌گراد در سطح ضعیف (07/14%) قرار گرفت. این یافته‌ها به صورت جامع تأیید می‌کنند که سویه بومیL. acidophilus TMPC 41127 دارای ویژگی‌های تکنولوژیکی مطلوبی است که آن را به یک کاندیدای قوی برای استفاده به عنوان کشت آغازگر در صنایع غذایی تخمیری و یک پروبیوتیک بالقوه در محصولات فراسودمند تبدیل می‌کند.
 
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Assessment of the Technological Properties of Lactobacillus acidophilus TMPC 41127 Isolated from Traditional Khiki Cheese

نویسندگان English

Behrooz Alizadeh behbahani 1
Parisa Ghasemi 2
Alireza Vasiee 3
Zeinab Mousavi 4
1 Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 PhD student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
3 Assistant Professor, Department of Food Safety and Quality Control, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran.
4 M.Sc. student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

In this study, the technological characteristics of the probiotic strain Lactobacillus acidophilus TMPC 41127, isolated from Khiki cheese, were investigated to evaluate its potential for industrial applications. The assessed characteristics included thermal resistance, exopolysaccharide (EPS) production, acidifying ability, proteolytic activity, and autolytic activity. The results showed that the L. acidophilus TMPC 41127 strain exhibited acceptable thermal resistance, with the highest survival rate (53.74%) recorded at 50°C. The strain was also classified as a strong acidifier, as it was able to reduce the initial pH of 6.49 to 4.85 within 24 hours. Furthermore, it demonstrated a significant capacity for producing mucoid EPS. Its proteolytic activity was confirmed by the formation of a halo with a diameter of 22.03 mm. Finally, the strain's autolytic activity was at a moderate level (20.21%) at 4°C and a weak level (14.07%) at -20°C. These findings collectively confirm that the native L. acidophilus TMPC 41127 strain possesses desirable technological traits, making it a strong candidate for use as a starter culture in the fermented food industry and as a potential probiotic in functional foods.
 

کلیدواژه‌ها English

Lactobacillus acidophilus TMPC 41127
Technological characteristics
Proteolytic activity
Exopolysaccharide
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