مجله علوم و صنایع غذایی ایران

مجله علوم و صنایع غذایی ایران

بررسی خواص ضددیابتی، ضدفشارخون و ضداکسیدانی پپتیدهای زیست فعال استخراج شده از ضایعات میگوی ببری سبز (Penaeus semisulcatus)

نوع مقاله : مقاله پژوهشی

نویسندگان
دانشگاه تربیت مدرس
10.48311/fsct.2026.83919.0
چکیده
در صنایع عمل‌آوری میگو حدود 45-40% از ماده اولیه به صورت مواد زائد شامل سر، امعا و احشا و پوسته دور ریز می‌شوند. هدف از این مطالعه، استخراج و ارزیابی فعالیت زیستی پپتیدهای حاصل از ضایعات فرآوری میگوی ببری سبز(Penaeus semisulcatus) بود. ضایعات حاصل از عمل‌آوری میگو تهیه و بافت های هدف جهت تیمار آنزیمی مورد استفاده قرار گرفت. نتایج مربوط به تعیین ترکیب تقریبی ماده خام اولیه بر اساس وزن خشک نمونه با رطوبت 74،5%، بیش ترین ترکیب بدن میگوی ببری سبز و سپس44% پروتئین و2/2% خاکستر،  محتوای بافت میگوی سبز را تشکیل می‌دادند. در ادامه، از سه نوع آنزیم آلکالین پروتئاز، آلکالاز و اواتاز جهت هیدرولیز و تولید پپتیدها از ضایعات میگو استفاده شد. سپس آنزیم‌های درونی میگو بوسیله دمای 85 درجه غیرفعال شده و در محدوده  pH 8/0 تنظیم گردید. هیدرولیز در 8 و 16 ساعت انجام شد و میزان پروتئین کلی بخش رویی توسط تکنیک کجدال، جهت آنالیز و بررسی نمونه‌های تولیدشده و انتخاب بهترین نمونه، سنجیده شد و ساعت 16 هیدرولیز شده با آنزیم آلکالاز با 35،5% پروتئین به عنوان نمونه‌ی بهینه انتخاب شد .پروتئین‌های استخراج‌شده با استفاده از آنزیم آلکالاز، هیدرولیز شده و در سه بازه وزنی کمتر از 3، 10 و 30 کیلو دالتون با روش اولترافیلتراسیون جداسازی شدند. نتایج نشان دادند که فراکسیون پپتیدی با وزن مولکولی کمتر از 3 kDa بیشترین فعالیت زیستی را داراست؛ به‌طوری‌ که مهار آنزیم DPP-IV تا 73.35٪، مهار ACE تا 55٪ و فعالیت مهار رادیکال‌ آزاد در آزمون DPPH برابر با 69.61٪ بود
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Investigation of Antidiabetic, Antihypertensive, and Antioxidant Properties of Bioactive Peptides Extracted from the Waste of Green Tiger Shrimp (Penaeus semisulcatus)

نویسندگان English

mahya damali amiri
reza hasan sajedi
tarbiat modares university
چکیده English

In shrimp processing industries, approximately 40-45% of the raw material is discarded as waste, including heads, viscera and, shells,. The aim of this study was to extract and evaluate the biological activity of peptides derived from the processing waste of the green tiger shrimp (Penaeus semisulcatus). The shrimp processing waste was prepared, and target tissues were used for enzymatic treatment. Results related to the approximate composition of the raw material based on dry weight with 74.5% moisture showed that the highest proportion was shrimp body tissue, followed by protein at 44% and ash at 2.2% in the green tiger shrimp tissue content. Subsequently, three types of enzymes—alkaline protease, Alcalase, and Ovataz—were used for hydrolysis and peptide production from shrimp waste. The endogenous shrimp enzymes were inactivated by heating at 85°C, and the pH was adjusted to 8/0. Hydrolysis was carried out for 8 and 16 hours, and the total protein content of the supernatant was measured using the Kjeldahl technique for analysis and selection of the best sample. The 16-hour hydrolysate treated with Alcalase, containing 35.5% protein, was selected as the optimal sample. The proteins extracted and hydrolyzed using Alcalase were separated by ultrafiltration into three molecular weight fractions: less than 3, 10, and 30 kDa. The results indicated that the peptide fraction with a molecular weight below 3 kDa exhibited the highest biological activity; specifically, inhibition of the DPP-IV enzyme up to 73.35%, ACE inhibition up to 55%, and free radical scavenging activity in the DPPH assay of 69.61%

کلیدواژه‌ها English

marine peptide
enzymatic hydrolysis
bioactive peptide
Green tiger shrimp
antidiabetic properties
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