اثرات سیتوتوکسیک انتروکوک های جدا شده از لبنیات سنتی ایران بر رده سلولی سرطان سینه SK-BR-3

نویسندگان
زیبا هرندی 1
کیوان بهشتی مآل 2
رامش منجمی 3
1 گروه میکروبیولوژی، دانشکده علوم زیستی، واحدفلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران
2 گروه میکروبیولوژی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران
3 گروه زیست شناسی، دانشکده علوم زیستی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران
10.22034/FSCT.21.155.198
چکیده
زمینه و هدف: سرطان پستان یک بیماری شایع و ناهمگن است که زنان را در سراسر جهان تحت تاثیر قرار می دهد. پروبیوتیک ها که به عنوان غذاهای کاربردی شناخته می شوند، به عنوان کاندیدهای بالقوه در مبارزه با سرطان سینه، هم در داخل بدن و هم در شرایط آزمایشگاهی معرفی شده اند. باکتری‌های اسید لاکتیک (LAB)، گروهی از پروبیوتیک‌ها، اثرات مفیدی بر سلامت میزبان دارند. این مطالعه با هدف غربالگری و شناسایی انتروکوک های پروبیوتیکی از پنیر سنتی ایرانی و شناسایی اثرات سیتوتوکسیک آن بر رده سلولی سرطان پستان SK-BR-3 انجام شد.

مواد و روش‌ها: باکتری های اسید لاکتیک از دو نمونه پنیر ایرانی پس از کشت و غنی‌سازی در براث MRS و سپس روی MRS آگار جداسازی شدند. جدایه های LAB با استفاده از آنالیزهای ماکروسکوپی، میکروسکوپی و مولکولی شناسایی شدند. سمیت سلولی LAB بر روی سلول‌های سرطانی SK-BR3 با استفاده از روش MTT با غلظت‌های باکتریایی 100، 250، 500 و 1000 میکروگرم بر میلی‌لیتر ارزیابی شد.

یافته‌ها: تجزیه و تحلیل مولکولی نشان داد که دو جدایه به‌دست‌آمده در این مطالعه ارتباط نزدیکی با گونه‌های انتروکوک دارند. آنها به عنوان Enterococcus faecalis HBM-IAUF-3 و Enterococcus hirae HBM-IAUF-5 تعیین شدند و توالی ژنوم 16s-rDNAآنها به ترتیب در GenBank، NCBI با شماره های دسترسی MG757697 و MG757702 ثبت شد. هر دو Enterococcus faecalis HBM-IAUF-3 و Enterococcus hirae HBM-IAUF-5 بیشترین سمیت سلولی را در برابر سلول های سرطانی SK-BR3 در غلظت 1000 میکرولیتر بر میلی لیتر پس از 72 ساعت نشان دادند.

نتیجه‌گیری: این مطالعه شناسایی سویه‌های جدید انتروکوکوس از لبنیات سنتی ایران را گزارش می‌کند که اثرات سیتوتوکسیک را بر روی سلول‌های SK-BR-3 سرطان پستان نشان می‌دهد. تشویق به مصرف لبنیات ارگانیک سنتی و غذاهای تخمیر شده محلی ممکن است خطر ابتلا به سرطان را به میزان قابل توجهی کاهش دهد.
کلیدواژه‌ها
آنتروکوکوس فکالیس
آنتروکوکوس هیرائه
پروبیونیک
سرطان سینه

موضوعات

عنوان مقاله English

Cytotoxic effects of Enterococci isolated from traditional Iranian dairy products on breast cancer cell line SK-BR-3

نویسندگان English

Ziba harandi 1
Keivan Beheshti-Maal 2
Ramesh Manajemi 3
1 Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2 Department of Microbiology, Faculty of Biological Sciences, falavarjan Branch, Islamic Azad University, Isfahan, Iran
3 Department of Biology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
چکیده English

Background and Aim: Breast cancer is a prevalent and heterogeneous disease affecting women worldwide. Probiotics, known as functional foods, have emerged as potential candidates in the fight against breast cancer, both in vivo and in vitro. Lactic Acid Bacteria (LAB), a group of probiotics, have been shown to have beneficial effects on host health and possess the potential to serve as adjuvant therapy for breast cancer prevention and treatment. This can be achieved through modulating immune responses and influencing the breast microbial community. This study aimed to screen and characterize probiotic Enterococci from Iranian traditional cheese and detect its cytotoxic effects on SK-BR-3 breast cancer cell line.

Materials and Methods: The LAB were isolated from two Iranian cheese samples after enrichment culture in MRS broth and then streak plate method on MRS agar. The LAB isolates were identified using macroscopic, microscopic, and molecular analyses. The cytotoxicity of LAB on SK-BR3 cancer cells was assessed using the MTT assay with bacterial concentrations of 100, 250, 500, and 1000 μg/ml.

Results: Molecular analysis revealed that the two isolates obtained in this study were closely related to Enterococcus species. They were designated as Enterococcus faecalis HBM-IAUF-3 and Enterococcus hirae HBM-IAUF-5, and their 16s-rDNA genome sequences were deposited in GenBank, NCBI under the accession numbers MG757697 and MG757702, respectively. Both Enterococcus faecalis HBM-IAUF-3 and Enterococcus hirae HBM-IAUF-5 exhibited the highest cytotoxicity against SK-BR3 cancer cells at a concentration of 1000 μl/ml after 72 hours.

Conclusion: This study reports the identification of novel Enterococcus strains from traditional Iranian dairy products that demonstrated cytotoxic effects on breast cancer SK-BR-3 cells. Encouraging the consumption of traditional organic dairy products and locally fermented foods may significantly reduce the risk of cancer.

کلیدواژه‌ها English

Breast Cancer
Enterococcus faecalis
Enterococcus hirae
probiotics
SK-BR-3 Cell Line
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