جداسازی و شناسایی سویه Limosilactobacillus fermentum ARD2 از ماست محلی و ارزیابی ویژگی‌های پروبیوتیکی، ضدمیکروبی و ایمنی آن

نویسندگان
بهروز علیزاده بهبهانی 1
محمدامین مهرنیا 1
حسین جوینده 2
فاطمه مطوری 3
1 1 دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان،
2 2 استاد، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
3 دانشجوی دکتری، گروه علوم و صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.22034/FSCT.21.154.184
چکیده
نقش مهم و اساسی غذا در زندگی، سبب روی آوردن به سمت محصولاتی شده است که علاوه بر دارا بودن خواص کیفی و تغذیه‌ای مطلوب، سلامت کلی مصرف‌کننده را نیز تضمین می‌کنند. پروبیوتیک‌ها میکروارگانیسم‌های غیربیماری‌زایی هستند که در صورت مصرف مقادیر کافی سبب برقراری تعادل میکروبی روده شده و سلامتی و ایمنی بدن را افزایش می‌دهند. در این پژوهش، ویژگی‌های پروبیوتیکی، ضدباکتریایی و ایمنی سویه Limosilactobacillus fermentum ARD2 جدا شده از ماست محلی بررسی شد. مقاومت به اسید (2، 3 و 4=pH)، مقاومت به نمک‌های صفراوی (3/0، 5/0 و 7/0 درصد)، فعالیت ضد میکروبی به روش دیسک و چاهک، مقاومت نسبت به آنتی‌بیوتیک‌های رایج درمانی، ارزیابی فعالیت آنتی‌اکسیدانی، هیدروفوبیستی سطح سلول، آنزیم DNase، فعالیت همولیتیک و آمین بیوژنیک و جذب کلسترول بررسی گردید. نتایج مقاومت به اسید نشان داد که تعداد سلول‌های زنده سویه L. fermentum ARD2 با افزایش pH از 2 به 4، افزایش یافته و با افزایش زمان از صفر به 3 ساعت در pH ثابت روند کاهشی مشاهده گردید. با افزایش غلظت نمک‌های صفراوی، رشد کاهش یافت. نتایج حاصل از اثر ضدمیکروبی نشان داد که در هر دو روش چاهک و دیسک سوپرناتانت اسیدی فاقد سلول (aCFS) و سوپرناتانت خنثی شده فاقد سلول (nCFS) روی Escherichia coli اثر ضد میکروبی نداشتند. nCFS فاقد اثر ضدمیکروبی بر Shigella dysenteriae بود. L. fermentum ARD2 به Ciprofloxacin مقاوم و نسبت به Penicilin و Nitrofurazone نیمه حساس بود. میزان مهار رادیکال آزاد DPPH و ABTS به ترتیب معادل 40/41 و 60/43 درصد بوده و این سویه توانست جذب کلسترول را به میزان 60/38 درصد کاهش دهد. نتیجه تست‌های DNase و فعالیت همولیتیکی منفی بود و تولید آمین بیوژنیک مشاهده نگردید. مطابق با نتایج به دست آمده L. fermentum ARD2 قابلیت پروبیوتیکی قابل قبولی داشته و می‌توان از این سویه در محصولات غذایی به عنوان یک باکتری پروبیوتیک استفاده نمود.
کلیدواژه‌ها
فعالیت آنتی‌اکسیدانی
فعالیت همولیتیک
آنتی‌بیوتیک‌های رایج درمانی
سوپرناتانت فاقد سلول

موضوعات

عنوان مقاله English

Isolation and identification of Limosilactobacillus fermentum ARD2 strain from local yogurt and evaluation of its probiotic, antimicrobial and safety properties

نویسندگان English

Behrooz Alizadeh behbahani 1
Mohammad Amin Mehrnia 1
Hossein Jooyandeh 2
Fatemeh Matori 3
1 1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 2Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
3 PhD. student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

The crucial role of food in life has prompted a shift towards products that not only offer good quality and nutritional properties but also ensure the overall health of consumers. Probiotics, which are non-pathogenic microorganisms, can restore intestinal microbial balance when consumed in sufficient quantities. In this study, Limosilactobacillus fermentum ARD2 isolated from local yogurt was investigated for its probiotic properties. Various aspects of this strain were evaluated, including acid resistance (at pH levels of 2, 3 and 4), resistance to bile salts (at concentrations of 0.3, 0.5 and 0.7 %), antimicrobial activity using disk diffusion agar and well diffusion agar methods, resistance to common therapeutic antibiotics, evaluation of antioxidant activity, cell surface hydrophobicity, DNase enzyme activity, hemolytic activity, and biogenic amine and cholesterol absorption. The results of the acid resistance tests indicated that the viability of L. fermentum ARD2 increased as pH levels rose from 2 to 4, with a decrease observed over time at a constant pH over 3 hours. Growth was inhibited with increasing concentrations of bile salts. Antimicrobial testing revealed that the acidic and neutralized cell-free supernatant (aCFS and nCFS) had no significant antimicrobial effect on Escherichia coli, while nCFS showed no antimicrobial effect on Shigella dysenteriae. L. fermentum ARD2 exhibited resistance to Ciprofloxacin and was semi-sensitive to Penicillin and Nitrofurazone. The L. fermentum ARD2 showed considerable antioxidant activity, with DPPH and ABTS free radical inhibition rates of 41.40% and 43.60%, respectively. Additionally, it demonstrated the ability to reduce cholesterol absorption by 38.60%. The strain tested negative for DNase and hemolytic activities, and biogenic amine production was not observed. Based on these findings, L. fermentum ARD2 exhibits promising probiotic characteristics and could be utilized as a probiotic bacterium in food products.

کلیدواژه‌ها English

Antioxidant activity
Hemolytic activity
Common therapeutic antibiotics
Cell-free supernatant
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