ارزیابی اثرات آنتی‌اکسیدانی عصاره آبی گیاه گزنه (Urtica dioica) بر مهار رادیکال‌های آزاد، سنجش محتوای فنول و فلاونوئید کل و تعیین گروه‌های عاملی زیست فعال

نویسندگان
پریسا قاسمی 1
بهروز علیزاده بهبهانی 2
محمد نوشاد 2
1 1. دانشجوی دکتری، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 2. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.22034/FSCT.21.154.163
چکیده
گیاه گزنه با نام علمیUrtica dioica L. ، گیاهی علفی و چند ساله است که در سرتاسر جهان گسترش یافته است. این گیاه سرشار از ترکیبات آنتی­اکسیدانی و دارای خاصیت ضدالتهاب، ضد سرطان و ضدحساسیت است. از این­رو، هدف از این مطالعه، تعیین گروه­های عاملی زیست فعال، محتوای فنول کل، فلاونوئید کل فعالیت آنتی­اکسیدان عصاره آبی برگ گیاه گزنه بود. جهت شناسایی گروه­های عاملی زیست فعال از روش طیف­سنجی تبدیل فوریه فروسرخ استفاده شد. همچنین برای اندازه­گیری محتوای فنولی کل از روش فولین-سیوکالتو، محتوای فلاونوئید کل از روش رنگ سنجی آلومنیوم کلراید، فعالیت آنتی­اکسیدانی به روش مهار رادیکال­های 2و2-دی فنیل 1- پیکریل هیدرازیل (DPPH) و 2،2- آزینو بیس-3- اتیل بنزو تیازولین – 6- سولفونیک اسید (ABTS) استفاده گردید. نتایج نشان داد که عصاره آبی برگ گزنه دارای mg GAE/g 83/59 فنول کل، mgQE/g 44/29 کل بود و فعالیت آنتی­اکسیدانی آن بر اساس مهار رادیکال آزاد DPPH و ABTS به ترتیب 47/66 و 32/81 درصد بدست آمد. این مطالعه نشان می‌دهد که عصاره آبی برگ گزنه منبع مهمی از آنتی‌اکسیدان‌های طبیعی است و می‌تواند جایگزین مناسبی برای آنتی‌اکسیدان‌های مصنوعی در نظر گرفته شود.
کلیدواژه‌ها
ترکیبات فنولی
گروه عاملی زیست فعال
گیاه گزنه
فعالیت آنتی‌اکسیدانی

موضوعات

عنوان مقاله English

Assessment of the Antioxidant Effects of Urtica dioica Aqueous Extract on Free Radical Scavenging, Determination of Total Phenol and Flavonoid Content, and the Bioactive Compound Groups

نویسندگان English

Parisa Ghasemi 1
Behrooz Alizadeh behbahani 2
Mohammad Noshad 2
1 1- Ph.D. Student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 2- Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

Urtica dioica L., commonly known as nettle is a perennial herbaceous plant that is widely distributed throughout the world. This plant is rich in antioxidant compounds and possesses anti-inflammatory, anticancer, and antiallergic properties. Therefore, the aim of this study was to determine the bioactive compound groups, total phenolic content, total flavonoid content, and antioxidant activity of aqueous leaf extract of nettle plant. Fourier-transform infrared spectroscopy (FTIR) was used for identifying the bioactive compound groups. Additionally, the total phenolic content was measured using the Folin-Ciocalteu method, the total flavonoid content was determined by the aluminum chloride colorimetric method, and the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The results showed that the aqueous leaf extract of nettle contained 59.83 mg GAE/g of total phenols, 29.44 mg QE/g of total flavonoids, and exhibited antioxidant activity with 66.47% and 81.32% inhibition of DPPH and ABTS radicals, respectively. This study demonstrates that the aqueous leaf extract of nettle is a significant source of natural antioxidants and can be considered as a suitable alternative to synthetic antioxidants in food and pharmaceutical industries.

کلیدواژه‌ها English

phenolic compounds
Bioactive compound groups
Nettle plant
Antioxidant activity
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