ریز پوشانی لاکتوباسیلوس اسیدوفیلوس توسط صمغ آمونیاکوم غنی شده با نانوسلنیوم: بررسی میزان زنده مانی در شرایط شبیه سازی شده دستگاه گوارش و دوره نگهداری

نویسندگان
عزیز همایونی راد 1
بهزاد ابراهیمی 2
1 استاد گروه علوم و صنایع غذایی، دانشکده تغذیه و علوم غذایی، دانشگاه علوم پزشکی تبریز، تبریز، ایران
2 استادیار گروه علوم و صنایع غذایی، دانشکده علوم پزشکی مراغه، مراغه، ایران
10.22034/FSCT.21.154.66
چکیده
افزایش تقاضا برای هیدروکلوئیدهای با کارآیی ویژه، محققان را برای معرفی منابع جدید هیدروکلوئیدی علاقه مند کرده است. یکی از روش­های افزایش زنده­مانی پروبیوتیک­ها در شرایط استرس­زا ریز پوشانی می باشد، تا بتوان این باکتری­ها را به تعداد کافی به بدن رساند و از اثرات سلامت بخش آنها استفاده کرد. هدف از این مطالعه بررسی امکان سنجی ریزپوشانی پروبیوتیک ل. اسیدوفیلوس توسط صمغ آمونیاکوم و غنی کردن آن با استفاده از نانوسلنیوم و تعیین میزان زنده مانی آن در شرایط استرس زا می باشد. صمغ آمونیاکوم با استفاده از حلال و سپس ترسیب توسط الکل از موسیلاژ حاصل از گیاه ­Dorema Ammonacum استخراج شد. محلول­های 1، 5 و 10­% وزنی- حجمی از صمغ آمونیاکوم تهیه شد و سپس نانو ذرات سلنیوم سنتز شده در غلظت 5/1 درصد و سلول باکتری با میانگین تعداد log cfu 1011 × 85/6، به سوسپانسیون اضافه شدند. نتایج نشان داد که با افزایش غلظت صمغ بازده انکپسولاسیون به صورت معنی­داری از 66% به 81% افزایش پیدا کرد. نرخ زنده­مانی برای غلظت­های 1­%، 5­% و 10­% صمغ آمونیاکوم در شرایط یخچالی به ترتیب 43/62%، 37/72­% و 83/81­% بود. در شرایط شبیه سازی شده معده در سطوح pH مورد استفاده در این مطالعه در غلظت­های 5­% و 10­% از صمغ آمونیاکوم شمارش سلول­های زنده پس از 3 ساعت انکوباسیون بالاتر از log cfu g-1 7 باقی ماند. پس از 6 ساعت انکوباسیون در g L-1 10 محلول صفرایی سلول های آزاد افت log cfu g-1 93/5 را نشان دادند، در حالی که برای سلول­های ریزپوشانی شده در غلظت­های 1 %، 5 % و10 % به ترتیب تنها log cfu g-1 93/3، log cfu g-1 15/3 و log cfu g-1 9/1بود. نتایج نشان داد صمغ آمونیاکوم خواص انکپسوله کنندگی بسیار خوبی دارد که می­توان از آن در بسیاری از فرمولاسیون­های غذایی با این اهداف مورد استفاده قرار داد.
کلیدواژه‌ها
صمغ آمونیاکوم
ریز پوشانی
نانو سلنیوم

موضوعات

عنوان مقاله English

Microencapsulation of Lactobacillus acidophilus by ammoniacum gum enriched with nanoselenium : investigating the survival rate in simulated gastrointestinal tract and storage period

نویسندگان English

Aziz Homayouni Rad 1
Behzad Ebrahimi 2
1 Professor, Departmentof Food Science and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Assistant Professor, Department of Food Science and Technology, Maragheh University of Medical Sciences, Maragheh, Iran
چکیده English

The escalating demand for hydrocolloids exhibiting exceptional performance has encouraged the interest of researchers in identifying novel sources of these hydrocolloids. Encapsulation has emerged as a strategy to boost the survivability of probiotics in harsh environmental conditions, enabling these beneficial bacteria to be transported to the body in sufficient quantities to leverage their health-promoting effects. This study aimed to explore the viability of microencapsulating probiotic L. acidophilus using ammoniacum gum (AMG), enhancing it with nanoselenium, and ascertaining its survival rate in stressful conditions. AMG, extracted from the mucilage of the Dorema Ammonacum plant using solvent and alcohol precipitation, was employed in the study. Solutions comprising 1%, 5%, and 10% weight-volume of AMG were prepared, followed by the synthesis of selenium nanoparticles at a concentration of 1.5%. Bacterial cells with an average count of 6.85×1011 log CFU were then introduced into the suspension. Observations revealed a substantial increase in encapsulation efficiency, rising from 66% to 81% with the escalation of gum concentration. The survival rates under refrigerated conditions for 1%, 5%, and 10% concentrations of AMG were recorded at 62.43%, 72.37%, and 81.83%, respectively. In simulated stomach conditions, at the pH levels applied in this study, concentrations of 5% and 10% of AMG exhibited sustained live cell counts exceeding 7 log cfu g-1 after 3 hours of incubation. Additionally, after a 6-hour incubation in a 10 g L-1 bile solution, free cells exhibited a reduction of 5.93 log cfu g-1, whereas micro-encapsulated cells at concentrations of 1%, 5%, and 10% demonstrated reductions of only 3.93 log cfu g-1, 3.15 log cfu g-1, and 1.9 log cfu g-1, respectively. These findings underscore the excellent encapsulation properties of AMG, locating it as a promising candidate for integration into numerous food formulations with similar objectives.

کلیدواژه‌ها English

nano selenium
amuniacum gum
microincapsulation
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