مطالعه فیلم زیست تخریب پذیر پلی لاکتیک اسید حاوی نانو ذرات نقره سنتز شده از عصاره مرزه ریشنگری بر خواص فیزیکوشیمیایی و میکروبی فیله مرغ در دمای یخچالی

نویسندگان
1 دانش آموخته کارشناسی ارشد گروه بهداشت مواد غذایی دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران
2 استادیار گروه بهداشت مواد غذایی دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران
3 دانشیار، گروه کنترل کیفی و بهداشت مواد غذایی، دانشکده علوم دامپزشکی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران
چکیده
عصاره های گیاهی و نانوذرات تهیه شده از آن ها به جهت دارا بودن خواص ضد میکروبی و آنتی اکسیدانی می توانند در افزایش مدت ماندگاری گوشت مورد استفاده قرار گیرند. در تحقیق حاضر تاثیر فیلم های پلی لاکتیک اسید حاوی نانو ذرات نقره سنتز شده از عصاره مرزه ریشنگری بر خواص فیزیکوشیمیایی و میکروبی فیله مرغ در دمای یخچالی در بازه های زمانی 0، 3، 7و 14 روز مورد بررسی قرار گرفت. نمونه ها شامل شاهد (کد1)، فیله مرغ پوشش دهی شده با فیلم پلی لاکتیک اسید (کد2)، فیله مرغ پوشش دهی شده با فیلم پلی لاکتیک اسید حاوی عصاره مرزه ریشنگری (کد3) و فیله مرغ پوشش دهی شده با فیلم پلی لاکتیک اسید حاوی نانو ذرات نقره سنتز شده از عصاره مرزه ریشنگری (کد4) بودند. نتایج نشان داد که میانگین قطر هاله عدم رشد بر علیه باکتری­های استافیلوکوکوس اورئوس و اشریشیاکلی برای عصاره مرزه حاوی نانوذرات نقره به طور معنی داری بالاتر ازعصاره مرزه بود (05/0p≤). در تمامی روزهای مورد بررسی به جز روز یکم، پائین ترین میزان pH وتیوباربیتوریک اسید متعلق به نمونه 4 بود (05/0p≤). در روزهای سوم و هفتم، بالاترین مولفه رنگیL* متعلق به نمونه 4 بود (05/0p≤). در تمامی روزهای مورد بررسی به جز در روز یکم، پائین ترین جمعیت باکتری­های مزوفیل، سایکروفیل، کلی فرم، استافیلوکوکوس اورئوس و کپک و مخمر و کپک و مخمر و همچنین بالاترین امتیاز کلیه فاکتورهای حسی (بو، رنگ، بافت، پذیرش کلی) متعلق به نمونه 4 بود (05/0p≤). نمونه 4 به جهت امتیاز حسی بالاتر و ویژگی های میکروبی مطلوب تر به عنوان تیمار برتر انتخاب شد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Study of the biodegradable film of polylactic acid containing silver nanoparticles synthesized from Rishnigari savory extract on the physicochemical and microbial properties of chicken fillet at refrigerated temperature.

نویسندگان English

Mina Tazakori 1
Amirali Amiralianvar 2
Hasan Hamedi 3
Nakisa Sohrabi Haqdoost 2
1 Msc Graduated, Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran (IAUPS)
2 Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Associate Professor, Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch ,Islamic Azad university , Tehran-Iran (IAUPS)
چکیده English

Plant extracts and nanoparticles prepared from them due to their antimicrobial and antioxidant properties can be used to increase the shelf life of meat. In the present study, the biodegradable film of polylactic acid containing silver nanoparticles synthesized from Satureja rechingeri extract on the physicochemical and microbial properties of chicken fillet at refrigerated temperature was investigated. So that the control sample (code 1), chicken fillet coated with biodegradable film of polylactic acid (code 2), chicken fillet coated with biodegradable film of polylactic acid containing Satureja rechingeri extract (code 3) and the Chicken fillet coated with biodegradable polylactic acid film containing silver nanoparticles synthesized from Satureja rechingeri extract (code 4) at 4°C in chemical, microbial and sensory properties at intervals of 0, 3, 7 and 14 The days were examined. The results showed that the mean diameter of the growth inhibition zone against Staphylococcus aureus and Escherichia coli bacteria for Satureja rechingeri extract containing silver nanoparticles was significantly higher than Satureja rechingeri extract (p≤0.05). In all the studied days except the first day, the lowest pH of thiobarbituric acid belonged to sample 4 (p≤0.05). Also, on the third and seventh days, the highest L* color component belonged to sample 4 (p≤0.05). On all the examined days, except on the first day, the lowest population of mesophilic bacteria, psychrophilic bacteria, coliform, Staphylococcus aureus, mold and yeast, and mold and yeast, as well as the highest score of all sensory factors (odor, color, texture, overall acceptance) belonged to sample 4 (p≤0.05).Sample 4 was selected as the superior treatment for higher sensory score and more desirable microbial properties.

کلیدواژه‌ها English

Polylactic acid
chicken fillet
Satureja rechingeri
silver nanoparticles
1) Fazal Ara, Maktabi, Najafzadeh Varzi, Ehsani, Adib, & Hassan. (2010). Determination of tetracycline residues in chicken meat consumed in Ahvaz city by high-performance liquid chromatography. Veterinary Laboratory Research, 2(2), 119-130.‎
2) Cerqueira, M. A., Bourbon, A. I., Pinheiro, A. C., Martins, J. T., Souza, B. W. S., Teixeira, J. A., & Vicente, A. A. (2011). Galactomannans use in the development of edible films/coatings for food applications. Trends in Food Science & Technology, 22(12), 662-671.
3) Jamshidian, M., Tehrany, E. A., Imran, M., Jacquot, M., & Desobry, S. (2010). Poly‐lactic acid: production, applications, nanocomposites, and release studies. Comprehensive reviews in food science and food safety, 9(5), 552-571.
4) Lunt, J. (1998). Large-scale production, properties and commercial applications of polylactic acid polymers. Polymer degradation and stability, 59(1-3), 145-152.
5) Suprakas S R, Pralay M, Masami O, Kazunobu Y, Kazue U., 2002. New polylactide/layered silicate nanocomposites. Preparation, characterization, and properties [J], Macromolecules, 35: 3104-3110.
6) Mohanty, A. K., Misra, M. A., & Hinrichsen, G. I. (2000). Biofibres, biodegradable polymers and biocomposites: An overview. Macromolecular materials and Engineering, 276(1), 1-24.
7) Lunt, J. (1998). Large-scale production, properties and commercial applications of polylactic acid polymers. Polymer degradation and stability, 59(1-3), 145-152.
8) Suprakas, L. Z., Ma, H. J., Zhu, X. S., Fan, Y. J., & Jin, Z. H. (2002). Preparation and properties of polylactide/nano‐silica in situ composites. Pigment & Resin Technology, 39(1), 27-31.
9) Mohanty, A. K., Misra, M. A., & Hinrichsen, G. I. (2000). Biofibres, biodegradable polymers and biocomposites: An overview. Macromolecular materials and Engineering, 276(1), 1-24.
10) Chun, S. Y., Kim, B. S., Lee, J. K., Lim, H. J., Bae, J. S., Chung, H. Y., ... & Kwon, T. G. (2012). Human amniotic fluid stem cell injection therapy for urethral sphincter regeneration in an animal model. BMC medicine, 10(1), 1-14.
11) Virkutyte, J., & Varma, R. S. (2011). Green synthesis of metal nanoparticles: biodegradable polymers and enzymes in stabilization and surface functionalization. Chemical Science, 2(5), 837-846.
12) Nadagouda, M. N., Iyanna, N., Lalley, J., Han, C., Dionysiou, D. D., & Varma, R. S. (2014). Synthesis of silver and gold nanoparticles using antioxidants from blackberry, blueberry, pomegranate, and turmeric extracts. ACS Sustainable Chemistry & Engineering, 2(7), 1717-1723.
13) Shipway, A. N., Katz, E., & Willner, I. (2000). Nanoparticle arrays on surfaces for electronic, optical, and sensor applications. ChemPhysChem, 1(1), 18-52.
14) Cho, S. H. (2005). Estimation of tumour dose enhancement due to gold nanoparticles during typical radiation treatments: a preliminary Monte Carlo study. Physics in Medicine & Biology, 50(15), N163.
15) Jiang, J., Oberdörster, G., & Biswas, P. (2009). Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies. Journal of Nanoparticle Research, 11(1), 77-89.
16) Klasen, H. J. (2000). Historical review of the use of silver in the treatment of burns. I. Early uses. Burns, 26(2), 117-130.
17) Sefidkan, F., and Taib Nia, R., and Mirza, M. (2015). Investigating the yield and essential oil compositions of six stands of Satureja rechingeri Jamzad in agricultural conditions. Iranian Medicinal and Aromatic Plants Research, 32(1)-12-24
18) Rasaee, I., Ghannadnia, M., & Baghshahi, S. (2018). Biosynthesis of silver nanoparticles using leaf extract of Satureja hortensis treated with NaCl and its antibacterial properties. Microporous and Mesoporous Materials: 264, p 240-247
19) Ozogul, Y., Yuvka, İ., Ucar, Y., Durmus, M., Kösker, A. R., Öz, M., & Ozogul, F. (2017). Evaluation of effects of nanoemulsion based on herb essential oils (rosemary, laurel, thyme and sage) on sensory, chemical and microbiological quality of rainbow trout (Oncorhynchus mykiss) fillets during ice storage.LWT-Food Science and Technology: 75, p 677-684.
20) Ed-Dra, A., Filali, F. R., Karraouan, B., El Allaoui, A., Aboulkacem, A., & Bouchrif, B. (2017). Prevalence, molecular and antimicrobial resistance of Salmonella isolated from sausages in Meknes, Morocco. Microbial pathogenesis, 105, 340-345.
21) Seif, M. and Jokar, M., 2013, production and spectroscopic analysis of polylactic acid film and polylactic acid/silver nanocomposite, the first electronic conference on new findings in the environment and agricultural ecosystems, Tehran
22) Fernández-López, J. A., Fernández-Fidalgo, M., & Cieza, A. (2010). Los conceptos de calidad de vida, salud y bienestar analizados desde la perspectiva de la Clasificación Internacional del Funcionamiento (CIF). Revista española de salud pública, 84, 169-184.
23) El Hamdaoui, A., Msanda, F., Boubaker, H., Leach, D., Bombarda, I., Vanloot, P., ... & El Mousadik, A. (2018). Essential oil composition, antioxidant and antibacterial activities of wild and cultivated Lavandula mairei Humbert. Biochemical Systematics and Ecology, 76, 1-7.
24) Narchin, F., Larijani, K., Rustaiyan, A., Ebrahimi, S. N., & Tafvizi, F. (2018). Phytochemical synthesis of silver nanoparticles by two techniques Using Saturaja rechengri Jamzad extract: identifying and comparing in Vitro anti-proliferative activities. Advanced Pharmaceutical Bulletin, 8(2), 235.
25) Bazargani-Gilani, B., Aliakbarlu, J., & Tajik, H. (2015). Effect of pomegranate juice dipping and chitosan coating enriched with Zataria multiflora Boiss essential oil on the shelf-life of chicken meat during refrigerated storage. Innovative food science & emerging technologies, 29, 280-287.
26) Firoozi, S., Jamzad, M., & Yari, M. (2016). Biologically synthesized silver nanoparticles by aqueous extract of Satureja intermedia CA Mey and the evaluation of total phenolic and flavonoid contents and antioxidant activity. Journal of Nanostructure in Chemistry: 6(4), p 357-364.
27) Hamzeh, A., Rezaei, M., 2011. Antioxidant and Antibacterial Effects of Sodium AlginateCoating Enrich with Thyme Essential Oil on Rainbow Trout Fillets During Refrigerator Storage. J. Iranian Nutrition Science and food Industry: 6(3), p 11-20.
28) He, X., & Hwang, H. M. (2016). Nanotechnology in food science: Functionality, applicability, and safety assessment. Journal of Food and Drug Analysis: 24(4), p 671–681.
29) Huang, X., Shipps, G. W., Cheng, C. C., Spacciapoli, P., Zhang, X., Mccoy, M. A., Wyss, D. F., Yang, X., Achab, A., Soucy, K., & Montavon, D. K. (2011). Discovery and hit-to-lead optimization of non-ATP cometitive MK2 (MAPKAPK2) inhibitors. ACS Medicinal Chemistry Letters: 2(8), p 632-637.
30) Seif, M. and Jokar, M., 2013, production and spectroscopic analysis of polylactic acid film and polylactic acid/silver nanocomposite, the first electronic conference on new findings in the environment and agricultural ecosystems, Tehran
31) Shabani, Sh., and Alimoradi, Sh. (2019). Investigating the antioxidant and antimicrobial effect of oak fruit powder on chicken meat stored in the refrigerator. Iran Food Sciences and Industries, 17(99), 55-69.
32) Khanehdan, N. (2011). Study of different concentration of sodium alginate as a coating film on the shelf-life of frozen dressed kilka (Clupeonella cultriventiris). Journal of American Science: 7(7), p 513-518.
33) kinga, Z., et al., 2015. Development and Validation of a Colorimetric sensor Array for FishSpoilage Monitoring. Journal of Food Control: 07, 038.
34) Macwan, D. P., Dave, P. N., & Chaturvedi, S. (2011). A review on nano-TiO2 sol–gel type syntheses and its applications. Journal of Materials Science: 46(11), p 3669-3686.
35) Oussalah, M., Caillet, S., Saucier, L., & Lacroix, M. (2007). Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E.coli O157: H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food control: 18(5), p 414-420.
36) -Pirbalouti, G. A., Broujeni, N. V., Momeni, M., Poor, M. F., & Hamedi, B. (2011). Antibacterial activity of Iranian medicinal plants against Streptococcus iniae isolated from rainbow trout (Oncorhynchus mykiss). Archives of Biological Sciences: 63(1), 59-66.
37) Rezaei, M. Pezeshk, S., et al., 2011. Effect of Antioxidant Activity of Shallot Extract (Alliumascalonicum), Turmeric Extract and their Composition on Changes of Lipids in Rainbow Trout(Oncorhychus mykiss) Vacuum Packaged. J. Fst.: 8(29), p 47-56.
38) Saharkhiz, M. J., Zomorodian, K., Taban, A., Pakshir, K., Heshmati, K., & Rahimi, M. J. (2016). Chemical Composition and Antimicrobial Activities of Three Satureja Species Against Food-borne Pathogens. Journal of Essential Oil Bearing Plants: 19(8), p 1984-1992.
39) Scholar, D. P., & Scholar, N. P. CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF SEVEN IRANIAN SATUREJA SPECIES-A REVIEW.
40) Shi, W., Song, S., & Zhang, H. (2013). Hydrothermal synthetic strategies of inorganic semiconducting nanostructures. Chemical Society Reviews: 42(13), p 5714-5743.
41) Thakkar, K. N., Mhatre, S. S., & Parikh, R. Y. (2010). Biological synthesis of metallic nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine: 6(2), p 257-262.
42) Tolaymat, T. M., El Badawy, A. M., Genaidy, A., Scheckel, K. G., Luxton, T. P., & Suidan, M. (2010). An evidence-based environmental perspective of manufactured silver nanoparticle in syntheses and applications: a systematic review and critical appraisal of peer-reviewed scientific papers. Science of the Total Environment: 408(5), p 999-1006.
43) Vafaei AA, MiladiGorji H, Taherian AA et al, 2010. Effects of Valeriane Officinalis, Satureja Hortensis, and Mentha Piperita extracts on the withdrawal syndrome signs in mice. Koomesh: 12 (3), p 342-347.
44) Cheng, J., Lin, X., Wu, X., Liu, Q., Wan, S., & Zhang, Y. (2021). Preparation of a multifunctional silver nanoparticles polylactic acid food packaging film using mango peel extract. International Journal of Biological Macromolecules, 188, 678-688.
45)
46) Gimenez, B., Roncales, P., & Beltran, J. A. (2002). Modified atmosphere packaging of filleted rainbow trout. Journal of the Science of Food and Agriculture, 82(10), 1154-1159
47) Lakshmanan, P. T. (2000). Fish spoilage and quality assessment.
48) López-Caballero, M. E., Gómez-Guillén, M. C., Pérez-Mateos, M., & Montero, P. (2005). A chitosan–gelatin blend as a coating for fish patties. Food Hydrocolloids, 19(2), 303-311.
49) Babuskin, S., Babu, P. A. S., Sasikala, M., Sabina, K., Archana, G., Sivarajan, M., & Sukumar, M. (2014). Antimicrobial and antioxidant effects of spice extracts on the shelf life extension of raw chicken meat. International journal of food microbiology, 171, 32-40.
50) -Afshar, P., & Sedaghat, S. (2016). Bio-synthesis of silver nanoparticles using water extract of Satureja hortensis L and evaluation of the antibacterial properties. Current Nanoscience: 12(1), p 90-93.
51) Ahmad, R., Mohsin, M., Ahmad, T., & Sardar, M. (2015). Alpha amylase assisted synthesis of TiO2 nanoparticles: structural- characterization and application as antibacterial agents. Journal of hazardous materials: 283, p 171-177.
52) Alghooneh, A., Behbahani, B. A., Noorbakhsh, H., & Yazdi, F. T. (2015). Application of intelligent modeling to predict the population dynamics of Pseudomonas aeruginosa in Frankfurter sausage containing Satureja bachtiarica extracts. Microbial pathogenesis: 85, p 58-65.
53) ANGİŞ, S., & OĞUZHAN, P. (2013). Effect of thyme essential oil and packaging treatments on chemical and microbiological properties of fresh rainbow trout (Oncorhynchus mykiss) fillets during storage at refrigerator temperatures. African Journal of Microbiology Research: 7(13), p 1136-1143.
54) Bukvički, D., Stojković, D., Soković, M., Vannini, L., Montanari, C., Pejin, B., ... & Marin, P. D. (2014). Satureja horvatii essential oil: In vitro antimicrobial and antiradical properties and in situ control of Listeria monocytogenes in pork meat. Meat Science: 96(3), p 1355-1360.
55) Carbone, M., Donia, D. T., Sabbatella, G., & Antiochia, R. (2016). Silver nanoparticles in polymeric matrices for fresh food packaging. Journal of King Saud University – Science: 28(4), p 273–279.
56) Choulitoudi, E., Bravou, K., Bimpilas, A., Tsironi, T., Tsimogiannis, D., Taoukis, P., & Oreopoulou, V. (2016). Antimicrobial and antioxidant activity of Satureja thymbra in gilthead seabream fillets edible coating. Food and Bioproducts Processing: 100, p 570-577.
57) -Cristiana, D. 2013. Quality index method to assess the freshness and shelf life of fish.Brazarch. Biotechnology: 56(4), p 587-598.
58) Ehsani, A., Paktarmani, M. and Yousefi, M. (2017) Efficiency of dietary sodium alginate coating incorporated with lycopene in preserving rainbow trout. Food Science and Biotechnology, 26,3, 557–562.
59) Erkan, N., Ozden, O., Alakavuk, DU., Yildirim, SY (2006) Spoilage and shelf life of sardines (Sardina pilchardus) packed in modified atmosphere. journal European Food Research and Technology, 222, 667-73.
60) Erbay, E. A., Dağtekin, B. B. G., Türe, M., Yeşilsu, A. F., and Torres-Giner, S (2017) Quality improvement of rainbow trout fillets by whey protein isolate coatings containing electrospun poly (ε-caprolactone) nanofibers with Urtica dioica L. extract during storage. Food Science and Technology, 78, 340-351.
61) Fadli, M., Chevalier, J., Saad, A., Mezrioui, N.E., Hassani, L., Pages, J.M (2012) Antibacterial activity of Thymus maroccanus and Thymus broussonetii essential oils against nosocomial infection −bacteria and their synergistic potential with antibiotics. Phytomedicine, 19, 464–471.
62) Fan, WJ., Sun,JX., Chen, YC., Qiu, J., Zhang, Y., Chi, YL (2009) Effects of chitosan coating on quality and shelf life of silver carp during frozen storage. Food chemistry, 115,1, 66-70.
63) Feng, LF., Jiang, TJ., Wang, YB., Li, JR (2012) Effects of tea polyphenol coating combined with ozone water washing on the storage quality of black sea bream (Sparus macrocephalus). Food chemistry, 135,4, 2915-2921.
64) Feng, X., Bansal, N., Yang, H (2016) Fish gelatin combined with chitosan coating inhibits myofibril degradation of golden pomfret (Trachinotus blochii) fillet during cold storage. Food chemistry, 200, 283-292.
65) Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing; 16th informational supplement (M100-S16). 2006. vol. 26, no. 3. CLSI, Wayne, Pa.
66) Dehghanizade, S., Arasteh, J., & Mirzaie, A. (2018). Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities. Artificial cells, nanomedicine, and biotechnology: 46(1), p 160-168.