ارزیابی ویژگی‌های پروبیوتیکی، ضدباکتریایی و ایمنی سویهLacticaseibacillus rhamnosus JCM 1136

نویسندگان
بهروز علیزاده بهبهانی 1
حسین جوینده 2
پگاه نمازی 3
1 1. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
2 2. استاد، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
3 3. دانشجوی دکتری، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
10.22034/FSCT.21.149.223
چکیده
پروبیوتیک‌ها باکتری‌های غیربیماری‌زا و مفیدی هستند که موجب بهبود سلامت دستگاه گوارش، ممانعت از ابتلا به انواع سرطان، تقویت سیستم ایمنی بدن و ... می‌شوند. هدف از پژوهش حاضر، مطالعه و بررسی پتانسیل پروبیوتیکی و فعالیت ضدمیکروبی سویه Lacticaseibacillus rhamnosus JCM 1136 بود. در این پژوهش، ویژگی‌های پروبیوتیکی و ایمنی سویه شامل مقاومت به اسید در PHهای ۲، ۳ و ۴، مقاومت به صفرا (صفر، ۳/۰، ۵/۰ و ۷/۰ درصد غلظت نمک صفراوی)، هیدروفوبیستی، پتانسیل تولید آنزیم DNase و آمین‌های بیوژنیک، میزان فعالیت همولیتیک، میزان فعالیت آنتی‌اکسیدانی، میزان جذب کلسترول، مقدار مقاومت به آنتی‌بیوتیک‌های رایج بررسی شد. اثر ضدمیکروبی سویه علیه پاتوژن‌های بیماری‌زا (Shigella dysenteriae، Salmonella typhimurium، Escherichia coli، Staphylococcus aureus، Listeria innocua و Bacillus cereus) به‌روش انتشار در آگار به‌کمک چاهک و دیسک ارزیابی گردید. نتایج نشان داد سویه L. rhamnosus JCM 1136 توانایی زنده‌مانی در PHهای مختلف را داشت. میزان رشد سویه با افزایش غلظت نمک صفراوی کاهش یافت. L. rhamnosus JCM 1136 نسبت به آنتی‌بیوتیک‌ Ampicillin نیمه‌حساس و نسبت به سایر آنتی‌بیوتیک‌ها حساس بود. هیدروفوبیستی، فعالیت آنتی‌اکسیدانی به روش های DPPH و ABTS و میزان جذب کلسترول سویه به ترتیب ۵۰/۰ ± ۵۰/۵۳ درصد، ۵۰/۰ ± ۲۳/۴۴، ۶۲/۰ ± ۵۰/۴۸ درصد و ۴۳/۰ ± ۵۵/۴۱ درصد بود. هیچگونه تولید آنزیم DNase، آمین‌های بیوژنیک و فعالیت همولیتیک از سویه مشاهده نشد. L. rhamnosus JCM 1136 دارای اثر ضدمیکروبی بیشتری بر باکتری‌های گرم مثبت بود. نتایج نشان داد که L. rhamnosus JCM 1136 دارای ویژگی‌های پروبیوتیکی مطلوب می‌باشد و قابلیت کاربرد در تولید محصولات غذایی پروبیوتیکی را نیز دارد.
کلیدواژه‌ها
باکتری‌های اسید لاکتیک
فعالیت همولیتیک
فعالیت ضدمیکروبی
آمین‌های بیوژنیک

موضوعات

عنوان مقاله English

Evaluation of probiotic, antibacterial and safety properties of Lacticaseibacillus rhamnosus JCM 1136

نویسندگان English

Behrooz Alizadeh behbahani 1
Hossein Jooyandeh 2
Pegah Namazi 3
1 1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 2Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
3 3PhD. student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

Probiotics are non-pathogenic and beneficial bacteria that contribute to improving digestive health, preventing various types of cancer, and boosting the body's immune system, among other benefits. The aim of this study was to investigate the probiotic potential and antimicrobial activity of the strain Lacticaseibacillus rhamnosus JCM 1136. In this research, the probiotic and safety characteristics of the strain were assessed, including acid resistance at pH 2, 3, and 4, bile resistance (0%, 0.3%, 0.5%, and 0.7% bile salt concentration), hydropHobicity, potential for producing DNase enzyme and biogenic amines, hemolytic activity, antioxidant activity, cholesterol absorption capacity, and resistance to common antibiotics. The antimicrobial effect of the strain against pathogenic bacteria (Shigella dysenteriae, Salmonella typhimurium, Escherichia coli, Staphylococcus aureus, Listeria innocua, and Bacillus cereus) was evaluated using the agar well diffusion and disk diffusion methods. The results showed that L. rhamnosus JCM 1136 exhibited survival capability at different pH levels. The growth of the strain decreased with increasing bile salt concentration. L. rhamnosus JCM 1136 showed intermediate to the antibiotic Ampicillin and sensitivity to other antibiotics. The hydropHobicity, antioxidant activity by DPPH and ABTS methods, and cholesterol absorption capacity of the strain were 50.0 ± 50.53%, 50.0 ± 23.44%, 62.0 ± 50.48%, and 43.0 ± 55.41%, respectively. No production of DNase enzyme, biogenic amines, or hemolytic activity was observed in the strain. L. rhamnosus JCM 1136 exhibited a greater antimicrobial effect against Gram-positive bacteria. The results indicate that L. rhamnosus JCM 1136 possesses desirable probiotic characteristics and can be used in the production of probiotic food products.

کلیدواژه‌ها English

lactic acid bacteria
Hemolytic activity
Antimicrobial activity
Biogenic amines
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